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Dev Biol 1990 Jan 01;1371:135-41. doi: 10.1016/0012-1606(90)90014-a.
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Molecular approach to dorsoanterior development in Xenopus laevis.

Sato SM , Sargent TD .

RNA from ventralized Xenopus laevis embryos has been used to generate a subtracted library consisting of genes whose expression is dependent upon dorsoanterior development. Clones were selected on the basis of two criteria: (1) at least 10-fold reduction in ventralized versus control embryo RNA, and (2) absence of hybridization to RNA from adult muscle. One of these clones, designated UVS.2, was characterized further. UVS.2, was found by in situ hybridization to be expressed exclusively in the anterior neural fold of neurula stage embryos. By the tailbud stage, the UVS.2 protein and transcript were localized in specialized cephalic ectoderm, in a region probably corresponding to the hatching gland. UVS.2 expression could be induced in ectodermal explants by contact with dorsal mesodermal tissue. Furthermore, in lithium-treated embryos, which have exaggerated dorsoanterior structures, UVS.2 expression was increased four- to fivefold. A molecular marker such as UVS.2 will be useful in defining and studying the induction and organization of cephalic tissues.

PubMed ID: 1688538
Article link: Dev Biol

Species referenced: Xenopus laevis
Genes referenced: actl6a astl2c astl3a.1
Antibodies: Uvs.2 Ab1

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