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XB-ART-11594
Nucleic Acids Res 2000 Feb 15;284:E12. doi: 10.1093/nar/28.4.e12.
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A simplified method of generating transgenic Xenopus.

Sparrow DB , Latinkic B , Mohun TJ .


Abstract
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.

PubMed ID: 10648800
PMC ID: PMC102591
Article link: Nucleic Acids Res



References [+] :
Christian, Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus. 1993, Pubmed, Xenbase