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EMBO J
2000 Mar 01;195:1010-22. doi: 10.1093/emboj/19.5.1010.
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Dishevelled phosphorylation, subcellular localization and multimerization regulate its role in early embryogenesis.
Rothbächer U
,
Laurent MN
,
Deardorff MA
,
Klein PS
,
Cho KW
,
Fraser SE
.
???displayArticle.abstract??? Dishevelled (Dsh) induces a secondary axis and can translocate to the membrane when activated by Frizzleds; however, dominant-negative approaches have not supported a role for Dsh in primary axis formation. We demonstrate that the Dsh protein is post-translationally modified at the dorsal side of the embryo: timing and position of this regulation suggests a role of Dsh in dorsal-ventral patterning in Xenopus. To create functional links between these properties of Dsh we analyzed the influence of endogenous Frizzleds and the Dsh domain dependency for these characteristics. Xenopus Frizzleds phosphorylate and translocate Xdsh to the membrane irrespective of their differential ectopic axes inducing abilities, showing that translocation is insufficient for axis induction. Dsh deletion analysis revealed that axis inducing abilities did not segregate with Xdsh membrane association. The DIX region and a short stretch at the N-terminus of the DEP domain are necessary for axis induction while the DEP region is required for Dsh membrane association and its phosphorylation. In addition, Dsh forms homomeric complexes in embryos suggesting that multimerization is important for its proper function.
Adler,
Tissue polarity points from cells that have higher Frizzled levels towards cells that have lower Frizzled levels.
1997, Pubmed
Adler,
Tissue polarity points from cells that have higher Frizzled levels towards cells that have lower Frizzled levels.
1997,
Pubmed
Axelrod,
Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways.
1998,
Pubmed
,
Xenbase
Axelrod,
Interaction between Wingless and Notch signaling pathways mediated by dishevelled.
1996,
Pubmed
Bhanot,
A new member of the frizzled family from Drosophila functions as a Wingless receptor.
1996,
Pubmed
Bhat,
frizzled and frizzled 2 play a partially redundant role in wingless signaling and have similar requirements to wingless in neurogenesis.
1998,
Pubmed
Blitz,
Anterior neurectoderm is progressively induced during gastrulation: the role of the Xenopus homeobox gene orthodenticle.
1995,
Pubmed
,
Xenbase
Boutros,
Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling.
1998,
Pubmed
Brannon,
A beta-catenin/XTcf-3 complex binds to the siamois promoter to regulate dorsal axis specification in Xenopus.
1997,
Pubmed
,
Xenbase
Cadigan,
Wnt signaling: a common theme in animal development.
1997,
Pubmed
,
Xenbase
Cooper,
Frizzled regulation of Notch signalling polarizes cell fate in the Drosophila eye.
1999,
Pubmed
Couso,
Notch is required for wingless signaling in the epidermis of Drosophila.
1994,
Pubmed
Deardorff,
Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis.
1998,
Pubmed
,
Xenbase
Doyle,
Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ.
1996,
Pubmed
Fagotto,
Domains of axin involved in protein-protein interactions, Wnt pathway inhibition, and intracellular localization.
1999,
Pubmed
,
Xenbase
Fanto,
Asymmetric Notch activation specifies photoreceptors R3 and R4 and planar polarity in the Drosophila eye.
1999,
Pubmed
Gho,
Frizzled signalling controls orientation of asymmetric sense organ precursor cell divisions in Drosophila.
1998,
Pubmed
Hamada,
Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin.
1999,
Pubmed
Hawley,
Disruption of BMP signals in embryonic Xenopus ectoderm leads to direct neural induction.
1995,
Pubmed
,
Xenbase
Heasman,
Patterning the Xenopus blastula.
1997,
Pubmed
,
Xenbase
Heslip,
Shaggy and dishevelled exert opposite effects on Wingless and Decapentaplegic expression and on positional identity in imaginal discs.
1997,
Pubmed
Itoh,
A role for Xenopus Frizzled 8 in dorsal development.
1998,
Pubmed
,
Xenbase
Kennedy,
Origin of PDZ (DHR, GLGF) domains.
1995,
Pubmed
Kennerdell,
Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway.
1998,
Pubmed
Kishida,
DIX domains of Dvl and axin are necessary for protein interactions and their ability to regulate beta-catenin stability.
1999,
Pubmed
Klingensmith,
Conservation of dishevelled structure and function between flies and mice: isolation and characterization of Dvl2.
1996,
Pubmed
,
Xenbase
Koelle,
EGL-10 regulates G protein signaling in the C. elegans nervous system and shares a conserved domain with many mammalian proteins.
1996,
Pubmed
Larabell,
Establishment of the dorso-ventral axis in Xenopus embryos is presaged by early asymmetries in beta-catenin that are modulated by the Wnt signaling pathway.
1997,
Pubmed
,
Xenbase
Laurent,
The Xenopus homeobox gene twin mediates Wnt induction of goosecoid in establishment of Spemann's organizer.
1997,
Pubmed
,
Xenbase
Lee,
Characterization of mouse dishevelled (Dvl) proteins in Wnt/Wingless signaling pathway.
1999,
Pubmed
Li,
Dishevelled proteins lead to two signaling pathways. Regulation of LEF-1 and c-Jun N-terminal kinase in mammalian cells.
1999,
Pubmed
Li,
Axin and Frat1 interact with dvl and GSK, bridging Dvl to GSK in Wnt-mediated regulation of LEF-1.
1999,
Pubmed
,
Xenbase
Miller,
Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation.
1999,
Pubmed
,
Xenbase
Miller,
Signal transduction through beta-catenin and specification of cell fate during embryogenesis.
1996,
Pubmed
Moon,
From cortical rotation to organizer gene expression: toward a molecular explanation of axis specification in Xenopus.
1998,
Pubmed
,
Xenbase
Morais Cabral,
Crystal structure of a PDZ domain.
1996,
Pubmed
Moriguchi,
Distinct domains of mouse dishevelled are responsible for the c-Jun N-terminal kinase/stress-activated protein kinase activation and the axis formation in vertebrates.
1999,
Pubmed
,
Xenbase
Müller,
Wingless signaling in the Drosophila embryo: zygotic requirements and the role of the frizzled genes.
1999,
Pubmed
Nasevicius,
Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation.
1998,
Pubmed
Peters,
Casein kinase I transduces Wnt signals.
1999,
Pubmed
,
Xenbase
Polakis,
The oncogenic activation of beta-catenin.
1999,
Pubmed
Ponting,
Pleckstrin's repeat performance: a novel domain in G-protein signaling?
1996,
Pubmed
Ponting,
PDZ domains: targeting signalling molecules to sub-membranous sites.
1997,
Pubmed
Rothbächer,
Functional conservation of the Wnt signaling pathway revealed by ectopic expression of Drosophila dishevelled in Xenopus.
1995,
Pubmed
,
Xenbase
Rowning,
Microtubule-mediated transport of organelles and localization of beta-catenin to the future dorsal side of Xenopus eggs.
1997,
Pubmed
,
Xenbase
Ruel,
Drosophila shaggy kinase and rat glycogen synthase kinase-3 have conserved activities and act downstream of Notch.
1993,
Pubmed
Sakanaka,
Casein kinase iepsilon in the wnt pathway: regulation of beta-catenin function.
1999,
Pubmed
,
Xenbase
Schneider,
Beta-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos.
1996,
Pubmed
,
Xenbase
Semënov,
Human dishevelled genes constitute a DHR-containing multigene family.
1997,
Pubmed
Shi,
Expression of Xfz3, a Xenopus frizzled family member, is restricted to the early nervous system.
1998,
Pubmed
,
Xenbase
Slusarski,
Interaction of Wnt and a Frizzled homologue triggers G-protein-linked phosphatidylinositol signalling.
1997,
Pubmed
,
Xenbase
Smalley,
Interaction of axin and Dvl-2 proteins regulates Dvl-2-stimulated TCF-dependent transcription.
1999,
Pubmed
Sokol,
Dorsalizing and neuralizing properties of Xdsh, a maternally expressed Xenopus homolog of dishevelled.
1995,
Pubmed
,
Xenbase
Sokol,
Analysis of Dishevelled signalling pathways during Xenopus development.
1996,
Pubmed
,
Xenbase
Strutt,
The role of RhoA in tissue polarity and Frizzled signalling.
1997,
Pubmed
Sussman,
Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled.
1994,
Pubmed
Theisen,
dishevelled is required during wingless signaling to establish both cell polarity and cell identity.
1994,
Pubmed
Tsang,
Isolation and characterization of mouse dishevelled-3.
1996,
Pubmed
Watabe,
Molecular mechanisms of Spemann's organizer formation: conserved growth factor synergy between Xenopus and mouse.
1995,
Pubmed
,
Xenbase
Willert,
Casein kinase 2 associates with and phosphorylates dishevelled.
1997,
Pubmed
Wittbrodt,
Disruption of mesoderm and axis formation in fish by ectopic expression of activin variants: the role of maternal activin.
1994,
Pubmed
,
Xenbase
Yanagawa,
The dishevelled protein is modified by wingless signaling in Drosophila.
1995,
Pubmed
Yang,
Genomic organization of mouse Dishevelled genes.
1996,
Pubmed
Yang-Snyder,
A frizzled homolog functions in a vertebrate Wnt signaling pathway.
1996,
Pubmed
,
Xenbase
Zeng,
The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation.
1997,
Pubmed
,
Xenbase
