Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1985 Jun 01;8212:4018-22.
Show Gene links
Show Anatomy links
Torsional stress induces an S1 nuclease-hypersensitive site within the promoter of the Xenopus laevis oocyte-type 5S RNA gene.
Reynolds WF
,
Gottesfeld JM
.
???displayArticle.abstract???
The internal promoter of the Xenopus laevis oocyte-type 5S RNA gene is preferentially cleaved by S1 and Bal-31 nucleases in plasmid DNA. S1 nuclease sensitivity is largely dependent on supercoiling; however, Bal-31 cleaves within the 5S RNA gene in linear as well as in supercoiled DNA. The S1 nuclease-hypersensitive site is centered at position +48-52 of the gene at the 5' boundary of the promoter. A DNAase I-hypersensitive site is induced at this position upon binding of the transcription factor, TFIIIA, specific for the 5S RNA gene. The somatic-type 5S RNA gene promoter is not preferentially cleaved by S1 nuclease or Bal-31 nuclease in supercoiled DNA, nor does TFIIIA induce a DNase I site at position +50. This differential promoter response may be related to a 4-fold difference in TFIIIA affinity between the oocyte and somatic 5S RNA genes.
Dudler,
Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.
1984, Pubmed,
Xenbase
Dudler,
Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.
1984,
Pubmed
,
Xenbase
Elgin,
Anatomy of hypersensitive sites.
,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Evans,
S1-hypersensitive sites in eukaryotic promoter regions.
1984,
Pubmed
Glikin,
Escherichia coli single-strand binding protein stabilizes specific denatured sites in superhelical DNA.
1983,
Pubmed
Goding,
S1 sensitive sites in adenovirus DNA.
1983,
Pubmed
Hanas,
Binding of Xenopus transcription factor A to 5S RNA and to single stranded DNA.
1984,
Pubmed
,
Xenbase
Hentschel,
Homocopolymer sequences in the spacer of a sea urchin histone gene repeat are sensitive to S1 nuclease.
1982,
Pubmed
Kohwi-Shigematsu,
Detection of an altered DNA conformation at specific sites in chromatin and supercoiled DNA.
1983,
Pubmed
Larsen,
An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.
1982,
Pubmed
Luchnik,
Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.
1982,
Pubmed
Mace,
Association of an S1 nuclease-sensitive structure with short direct repeats 5' of Drosophila heat shock genes.
,
Pubmed
Miller,
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
1978,
Pubmed
,
Xenbase
Panayotatos,
Cruciform structures in supercoiled DNA.
1981,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Reynolds,
5S rRNA gene transcription factor IIIA alters the helical configuration of DNA.
1983,
Pubmed
,
Xenbase
Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase
Sakonju,
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
1981,
Pubmed
,
Xenbase
Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Schon,
Conformation of promoter DNA: fine mapping of S1-hypersensitive sites.
1983,
Pubmed
Singleton,
Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions.
1982,
Pubmed
Smith,
Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
1984,
Pubmed
,
Xenbase
Wormington,
A quantitative assay for Xenopus 5S RNA gene transcription in vitro.
1981,
Pubmed
,
Xenbase
Wormington,
Onset of 5 S RNA gene regulation during Xenopus embryogenesis.
1983,
Pubmed
,
Xenbase
Wu,
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
1980,
Pubmed
