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We have injected XIHbox 6 mRNA together with the lineage tracer colloidal gold into individual dorso-anterior blastomeres of the 32-cell stage Xenopus embryo and analyzed their cell fate during embryogenesis. While the developing tadpoles appeared entirely normal, the fate of the progeny of the injected blastomere was altered. In the brain injected cells failed to differentiate terminally, as indicated by a loss of labeled cranial nerves. Differentiation of spinal nerves remained unaffected. Fate change in the CNS occurred at about the time of normal XIHbox 6 protein expression. In addition, progeny of injected blastomeres gained head epidermal fate and lost anterior notochord fate as a result of altered cell migrations during gastrulation. The results show that a homeodomain protein is capable of altering cell fate in a position-specific and cell-autonomous manner in Xenopus embryos. The experimental approach used here should be applicable to other molecules specifying cell fate.
Balling,
Craniofacial abnormalities induced by ectopic expression of the homeobox gene Hox-1.1 in transgenic mice.
1989, Pubmed
Balling,
Craniofacial abnormalities induced by ectopic expression of the homeobox gene Hox-1.1 in transgenic mice.
1989,
Pubmed
Blumberg,
Organizer-specific homeobox genes in Xenopus laevis embryos.
1991,
Pubmed
,
Xenbase
Busa,
Lithium-induced teratogenesis in frog embryos prevented by a polyphosphoinositide cycle intermediate or a diacylglycerol analog.
1989,
Pubmed
,
Xenbase
Chisaka,
Regionally restricted developmental defects resulting from targeted disruption of the mouse homeobox gene hox-1.5.
1991,
Pubmed
Cho,
Differential utilization of the same reading frame in a Xenopus homeobox gene encodes two related proteins sharing the same DNA-binding specificity.
1988,
Pubmed
,
Xenbase
Cho,
Differential activation of Xenopus homeo box genes by mesoderm-inducing growth factors and retinoic acid.
1990,
Pubmed
,
Xenbase
Cho,
Overexpression of a homeodomain protein confers axis-forming activity to uncommitted Xenopus embryonic cells.
1991,
Pubmed
,
Xenbase
Dale,
Fate map for the 32-cell stage of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Dent,
A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus.
1989,
Pubmed
,
Xenbase
De Robertis,
Homeobox genes and the vertebrate body plan.
1990,
Pubmed
,
Xenbase
Desplan,
The sequence specificity of homeodomain-DNA interaction.
1988,
Pubmed
Gehring,
Homeo boxes in the study of development.
1987,
Pubmed
Gimlich,
Improved fluorescent compounds for tracing cell lineage.
1985,
Pubmed
,
Xenbase
Graham,
The murine and Drosophila homeobox gene complexes have common features of organization and expression.
1989,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Harvey,
Microinjection of synthetic Xhox-1A homeobox mRNA disrupts somite formation in developing Xenopus embryos.
1988,
Pubmed
,
Xenbase
Hoey,
Divergent homeo box proteins recognize similar DNA sequences in Drosophila.
1988,
Pubmed
Holgate,
Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.
1983,
Pubmed
Holt,
Cellular determination in the Xenopus retina is independent of lineage and birth date.
1988,
Pubmed
,
Xenbase
Jacobson,
Coupled instability of two X-linked genes in Drosophila mauritiana: germinal and somatic mutability.
1985,
Pubmed
Kageura,
Spatial distribution of the capacity to initiate a secondary embryo in the 32-cell embryo of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Keller,
Vital dye mapping of the gastrula and neurula of Xenopus laevis. I. Prospective areas and morphogenetic movements of the superficial layer.
1975,
Pubmed
,
Xenbase
Kessel,
Murine developmental control genes.
1990,
Pubmed
Kessel,
Variations of cervical vertebrae after expression of a Hox-1.1 transgene in mice.
1990,
Pubmed
Klein,
The first cleavage furrow demarcates the dorsal-ventral axis in Xenopus embryos.
1987,
Pubmed
,
Xenbase
Klein,
Lithium changes the ectodermal fate of individual frog blastomeres because it causes ectopic neural plate formation.
1989,
Pubmed
,
Xenbase
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Moody,
Fates of the blastomeres of the 32-cell-stage Xenopus embryo.
1987,
Pubmed
,
Xenbase
Oliver,
Differential antero-posterior expression of two proteins encoded by a homeobox gene in Xenopus and mouse embryos.
1988,
Pubmed
,
Xenbase
Rosa,
Mix.1, a homeobox mRNA inducible by mesoderm inducers, is expressed mostly in the presumptive endodermal cells of Xenopus embryos.
1989,
Pubmed
,
Xenbase
Ruiz i Altaba,
Interaction between peptide growth factors and homoeobox genes in the establishment of antero-posterior polarity in frog embryos.
1989,
Pubmed
,
Xenbase
Ruiz i Altaba,
Bimodal and graded expression of the Xenopus homeobox gene Xhox3 during embryonic development.
1989,
Pubmed
,
Xenbase
Scott,
The structure and function of the homeodomain.
1989,
Pubmed
Sharpe,
A homeobox-containing marker of posterior neural differentiation shows the importance of predetermination in neural induction.
1987,
Pubmed
,
Xenbase
Wei,
Cellular retinoic acid- and cellular retinol-binding proteins: complementary deoxyribonucleic acid cloning, chromosomal assignment, and tissue specific expression.
1987,
Pubmed
Wetts,
Slow intermixing of cells during Xenopus embryogenesis contributes to the consistency of the blastomere fate map.
1989,
Pubmed
,
Xenbase
Wright,
Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord.
1989,
Pubmed
,
Xenbase
Wright,
The Xenopus XIHbox 6 homeo protein, a marker of posterior neural induction, is expressed in proliferating neurons.
1990,
Pubmed
,
Xenbase
