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Mol Cell Biol
1993 Feb 01;132:1146-54. doi: 10.1128/mcb.13.2.1146-1154.1993.
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Differential regulation of two distinct families of glucose transporter genes in Trypanosoma brucei.
Bringaud F
,
Baltz T
.
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A tandemly arranged multigene family encoding putative hexose transporters in Trypanosoma brucei has been characterized. It is composed of two 80% homologous groups of genes called THT1 (six copies) and THT2 (five copies). When Xenopus oocytes are microinjected with in vitro-transcribed RNA from a THT1 gene, they express a glucose transporter with properties similar to those of the trypanosome bloodstream-form protein(s). This THT1-encoded transport system for glucose differs from the human erythrocyte-type glucose transporter by its moderate sensitivity to cytochalasin B and its capacity to transport D-fructose. These properties suggest that the trypanosomal transporter may be a good target for antitrypanosomal drugs. mRNA analysis revealed that expression of these genes was life cycle stage dependent. Bloodstream forms express 40-fold more THT1 than THT2. In contrast, procyclic trypanosomes express no detectable THT1 but demonstrate glucose-dependent expression of THT2.
Alexandre,
Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei.
1988, Pubmed
Alexandre,
Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei.
1988,
Pubmed
Baldwin,
Homologies between sugar transporters from eukaryotes and prokaryotes.
1989,
Pubmed
Baltz,
Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense.
1985,
Pubmed
Ben Amar,
Structure and transcription of the actin gene of Trypanosoma brucei.
1988,
Pubmed
Ben Amar,
The actin gene promoter of Trypanosoma brucei.
1991,
Pubmed
Bernards,
Activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an altered 3' end.
1981,
Pubmed
Boorer,
Functional expression of a plant plasma membrane transporter in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Borst,
Discontinuous transcription and antigenic variation in trypanosomes.
1986,
Pubmed
Bringaud,
A potential hexose transporter gene expressed predominantly in the bloodstream form of Trypanosoma brucei.
1992,
Pubmed
Cairns,
Developmentally regulated gene from Leishmania encodes a putative membrane transport protein.
1989,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Clayton,
Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei.
1990,
Pubmed
Eisenthal,
Specificity and kinetics of hexose transport in Trypanosoma brucei.
1989,
Pubmed
Game,
Sugar transport in Trypanosoma brucei: a suitable kinetic probe.
1986,
Pubmed
Gibson,
Post-transcriptional control of the differential expression of phosphoglycerate kinase genes in Trypanosoma brucei.
1988,
Pubmed
Gruenberg,
D-Glucose transport in Trypanosoma brucei. D-Glucose transport is the rate-limiting step of its metabolism.
1978,
Pubmed
Hohn,
Packaging recombinant DNA molecules into bacteriophage particles in vitro.
1977,
Pubmed
Hsu,
A developmentally regulated gene of trypanosomes encodes a homologue of rat protein-disulfide isomerase and phosphoinositol-phospholipase C.
1989,
Pubmed
Imboden,
Transcription of the intergenic regions of the tubulin gene cluster of Trypanosoma brucei: evidence for a polycistronic transcription unit in a eukaryote.
1987,
Pubmed
Kasahara,
Reconstitution and purification of the D-glucose transporter from human erythrocytes.
1977,
Pubmed
Kooter,
The anatomy and transcription of a telomeric expression site for variant-specific surface antigens in T. brucei.
1987,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kueng,
A glycosomal protein (p60) which is predominantly expressed in procyclic Trypanosoma brucei. Characterization and DNA sequence.
1989,
Pubmed
Lanham,
Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.
1970,
Pubmed
Mowatt,
Developmental regulation of a novel repetitive protein of Trypanosoma brucei.
1987,
Pubmed
Mueckler,
Sequence and structure of a human glucose transporter.
1985,
Pubmed
Munoz-Antonia,
Differences in glucose transport between blood stream and procyclic forms of Trypanosoma brucei rhodesiense.
1991,
Pubmed
Nielsen,
Preparation of capped RNA transcripts using T7 RNA polymerase.
1986,
Pubmed
Parsons,
Active transport of 2-deoxy-D-glucose in Trypanosoma brucei procyclic forms.
1990,
Pubmed
Pays,
Trypanosoma brucei: constitutive activity of the VSG and procyclin gene promoters.
1990,
Pubmed
Rolin,
Stage-specific adenylate cyclase activity in Trypanosoma brucei.
1990,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schell,
A transferrin-binding protein of Trypanosoma brucei is encoded by one of the genes in the variant surface glycoprotein gene expression site.
1991,
Pubmed
Seyfang,
Specificity of glucose transport in Trypanosoma brucei. Effective inhibition by phloretin and cytochalasin B.
1991,
Pubmed
Tanabe,
Glucose transport in malaria infected erythrocytes.
1990,
Pubmed
Ter Kuile,
Glucose uptake by Trypanosoma brucei. Rate-limiting steps in glycolysis and regulation of the glycolytic flux.
1991,
Pubmed
Torri,
Posttranscriptional regulation of cytochrome c expression during the developmental cycle of Trypanosoma brucei.
1988,
Pubmed
Tschudi,
Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.
1985,
Pubmed
Vera,
Functional expression of mammalian glucose transporters in Xenopus laevis oocytes: evidence for cell-dependent insulin sensitivity.
1989,
Pubmed
,
Xenbase
Vera,
Reconstitution of an insulin signaling pathway in Xenopus laevis oocytes: coexpression of a mammalian insulin receptor and three different mammalian hexose transporters.
1990,
Pubmed
,
Xenbase
Vijayasarathy,
The genes encoding fructose bisphosphate aldolase in Trypanosoma brucei are interspersed with unrelated genes.
1990,
Pubmed
Wirtz,
Characterization of a novel developmentally regulated gene from Trypanosoma brucei encoding a potential phosphoprotein.
1991,
Pubmed
Ziegelbauer,
Molecular characterization of two invariant surface glycoproteins specific for the bloodstream stage of Trypanosoma brucei.
1992,
Pubmed
Zilberstein,
Identification and biochemical characterization of the plasma membrane glucose transporter of Leishmania donovani.
1986,
Pubmed
Zilberstein,
Protonmotive force-driven active transport of D-glucose and L-proline in the protozoan parasite Leishmania donovani.
1985,
Pubmed