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XB-ART-52132
Mol Cell 2009 Mar 13;335:591-601.
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ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing.

Warzecha CC , Sato TK , Nabet B , Hogenesch JB , Carstens RP .


Abstract
Cell-type-specific expression of epithelial and mesenchymal isoforms of Fibroblast Growth Factor Receptor 2 (FGFR2) is achieved through tight regulation of mutually exclusive exons IIIb and IIIc, respectively. Using an application of cell-based cDNA expression screening, we identified two paralogous epithelial cell-type-specific RNA-binding proteins that are essential regulators of FGFR2 splicing. Ectopic expression of either protein in cells that express FGFR2-IIIc caused a switch in endogenous FGFR2 splicing to the epithelial isoform. Conversely, knockdown of both factors in cells that express FGFR2-IIIb by RNA interference caused a switch from the epithelial to mesenchymal isoform. These factors also regulate splicing of CD44, p120-Catenin (CTNND1), and hMena (ENAH), three transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). These studies suggest that Epithelial Splicing Regulatory Proteins 1 and 2 (ESRP1 and ESRP2) are coordinators of an epithelial cell-type-specific splicing program.

PubMed ID: 19285943
PMC ID: PMC2702247
Article link: Mol Cell


Species referenced: Xenopus
Genes referenced: cd44 ctnnd1 esrp1 fgfr2
Antibodies: Esrp1 Ab1

References [+] :
Allemand, Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock. 2005, Pubmed