Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
In Vitro Synthesis and Processing of Wheat alpha-Amylase : TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS.
Boston RS
,
Miller TJ
,
Mertz JE
,
Burgess RR
.
???displayArticle.abstract???
Wheat (Triticum aestivum) RNA was used to program synthesis of the alpha-amylase protein by Xenopus laevis oocytes. A 41,500-dalton protein was made which was identified as alpha-amylase by immunoprecipitation with rabbit anti-alpha-amylase antiserum raised against the purified wheat protein and by its co-migration with authentic alpha-amylase on sodium dodecyl sulfate polyacrylamide gels. Synthesis of alpha-amylase was dependent upon injection of RNA extracted from gibberellic acid-induced aleurone layers from wheat. The amount of alpha-amylase produced was proportional to the amount of RNA injected and reached a plateau within 4 hours after injection. When the same RNA was translated in a wheat germ cell-free translation system, a 43,000-dalton protein was produced. Addition of dog pancreas microsomal membranes to the wheat germ translation system resulted in processing of the alpha-amylase protein to a form which co-migrated with authentic alpha-amylase purified from malted wheat and with the protein synthesized in oocytes.
Asselbergs,
Kinetics of synthesis and processing of precursor polypeptides of murine leukemia virus in frog oocytes following microinjection of viral RNA.
1980, Pubmed,
Xenbase
Asselbergs,
Kinetics of synthesis and processing of precursor polypeptides of murine leukemia virus in frog oocytes following microinjection of viral RNA.
1980,
Pubmed
,
Xenbase
Bielinska,
Processing in vitro of placental peptide hormones by smooth microsomes.
1979,
Pubmed
Bielinska,
Processing of placental peptide hormones synthesized in lysates containing membranes derived from tunicamycin-treated ascites tumor cells.
1978,
Pubmed
Birken,
Partial amino acid sequence of human placental lactogen precursor and its mature hormone form produced by membrane-associated enzyme activity.
1977,
Pubmed
Blackburn,
Ribonuclease inhibitor from human placenta: rapid purification and assay.
1979,
Pubmed
Blobel,
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
1975,
Pubmed
Click,
The isolation of ribonucleic acid from plant, bacterial or animal cells.
1966,
Pubmed
Colman,
The influence of topology and glycosylation on the fate of heterologous secretory proteins made in Xenopus oocytes.
1981,
Pubmed
,
Xenbase
De Robertis,
Coupled transcription-translation of DNA injected into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Dicou,
Synthesis of Dictyostelium discoideum secretory proteins in Xenopus laevis oocytes.
1979,
Pubmed
,
Xenbase
Filner,
A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.
1967,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Hurkman,
Subcellular compartmentalization of maize storage proteins in Xenopus oocytes injected with zein messenger RNAs.
1981,
Pubmed
,
Xenbase
Jacobsen,
Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue.
1974,
Pubmed
Joseph,
Precursor Forms of Pea Vicilin Subunits: MODIFICATION BY MICROSOMAL MEMBRANES DURING CELL-FREE TRANSLATION.
1981,
Pubmed
Katz,
Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein.
1977,
Pubmed
Kessler,
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
1975,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lane,
The sequestration, processing and retention of honey-bee promelittin made in amphibian oocytes.
1981,
Pubmed
,
Xenbase
Lane,
The Xenopus oocyte as a surrogate secretory system. The specificity of protein export.
1980,
Pubmed
,
Xenbase
Larkins,
Synthesis and processing of maize storage proteins in Xenopus laevis oocytes.
1979,
Pubmed
,
Xenbase
Laskey,
Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis.
1972,
Pubmed
,
Xenbase
Mozer,
Partial purification and characterization of the mRNA for alpha-amylase from barley aleurone layers.
1980,
Pubmed
Mozer,
Control of protein synthesis in barley aleurone layers by the plant hormones gibberellic acid and abscisic acid.
1980,
Pubmed
Nagata,
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.
1980,
Pubmed
Okita,
Synthesis of a possible precursor of alpha-amylase in wheat aleurone cells.
1979,
Pubmed
Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed
Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Rapoport,
Synthesis of carp proinsulin in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Roberts,
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
1973,
Pubmed
Schröder,
Translation of plant-specific messenger RNAs in living animal cells.
1977,
Pubmed
,
Xenbase
Shields,
Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes.
1978,
Pubmed
Shields,
Cell-free synthesis of fish preproinsulin, and processing by heterologous mammalian microsomal membranes.
1977,
Pubmed
Telford,
Delimitation of a promoter for RNA polymerase III by means of a functional test.
1979,
Pubmed
,
Xenbase
Vassart,
Thyroglobulin messenger RNA: translation of a 33-S mRNA into a peptide immunologically related to thyroglobulin.
1975,
Pubmed
,
Xenbase
