XB-ART-35846
Proc Natl Acad Sci U S A
1969 Jun 01;632:378-83. doi: 10.1073/pnas.63.2.378.
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Formation and detection of RNA-DNA hybrid molecules in cytological preparations.
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A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus. A low level of gene amplification was also detected in premeiotic nuclei (oogonia).
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References [+] :
BAUTZ,
The isolation of T4-specific RNA on a DNA-cellulose column.
1962, Pubmed