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Cold Spring Harb Protoc 2022 Oct 25; doi: 10.1101/pdb.top106195.
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Obtaining Xenopus Eggs and Embryos.

Sive HL , Harland RM .

Collecting eggs from adult Xenopus laevis and Xenopus tropicalis to raise healthy embryos and tadpoles is relatively simple but requires careful handling of the frog. Eggs can be fertilized through natural matings or by in vitro fertilization and examined visually. Here we review how eggs are obtained and how to recognize healthy eggs that will develop into high-quality embryos.

PubMed ID: 36283836
Article link: Cold Spring Harb Protoc