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Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.
May FE
,
Weber R
,
Westley BR
.
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The blood of the frog X.laevis contains 2 albumins of 68,000 and 74,000 daltons which are encoded in the liver by two related mRNAs. When an amplified X.laevis DNA library was screened with cloned albumin cDNA only 68,000 dalton albumin gene sequences were isolated. Hybridisation of the albumin cDNA to Southern-blots of Eco R1 digested X.laevis DNA showed that the sequences present in the recombinants did not account for all the fragments which hybridised on the Southern-blots. This indicated that 74K albumin gene sequences exist but that they are not present in the amplified DNA library. A X.laevis genomic library was therefore constructed and screened for albumin genes without amplification. Both 68K and 74K albumin gene sequences were isolated. Recombinants containing 74K albumin gene sequences grew extremely poorly and this probably explains why the 74K albumin sequences were ot isolated from the amplified library. Characterisation of the cloned DNA indicates that there is one sequence coding for the 68K albumin but two different sequences coding for the 75K albumin.
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977, Pubmed,
Xenbase
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Bozzoni,
Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.
1981,
Pubmed
,
Xenbase
Davis,
Rapid DNA isolations for enzymatic and hybridization analysis.
1980,
Pubmed
Dolan,
Chromosomal arrangement of the chicken beta-type globin genes.
1981,
Pubmed
Efstratiadis,
The structure and evolution of the human beta-globin gene family.
1980,
Pubmed
Fritsch,
Molecular cloning and characterization of the human beta-like globin gene cluster.
1980,
Pubmed
Herrlich,
The endogenous proviral mouse mammary tumor virus genes of the GR mouse are not identical and only one corresponds to the exogenous virus.
1981,
Pubmed
Kioussis,
The evolution of alpha-fetoprotein and albumin. II. The structures of the alpha-fetoprotein and albumin genes in the mouse.
1981,
Pubmed
Maniatis,
The isolation of structural genes from libraries of eucaryotic DNA.
1978,
Pubmed
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Ryffel,
Comparison of cytoplasmic and nuclear poly(A)-containing RNA sequences in Xenopus liver cells.
1976,
Pubmed
,
Xenbase
Sargent,
The rat serum albumin gene: analysis of cloned sequences.
1979,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Wahli,
Vitellogenin in Xenopus laevis is encoded in a small family of genes.
1979,
Pubmed
,
Xenbase
Wahli,
Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
1980,
Pubmed
,
Xenbase
Wahli,
Vitellogenesis and the vitellogenin gene family.
1981,
Pubmed
,
Xenbase
Westley,
Xenopus laevis serum albumins are encoded in two closely related genes.
1981,
Pubmed
,
Xenbase
