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In Vitro Cell Dev Biol Anim
2003 Jan 01;391-2:71-9. doi: 10.1290/1543-706X(2003)039<0071:PADOXA>2.0.CO;2.
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Proliferation and differentiation of Xenopus A6 cells under hypergravity as revealed by time-lapse imaging.
Tanaka M
,
Asashima M
,
Atomi Y
.
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Xenopus laevis A6 cells, which are cloned epithelial cells from the Xenopus kidney, differentiate into a dome structure when the cells reach confluence. We investigated the gravitational responses of A6 cellular motility during normal differentiation and differentiation under hypergravity conditions using centrifugation (1-100 x g). Progression to dome formation was analyzed by time-lapse micrography. Dome formation and increased expression of Na(+)/K(+)-adenosine triphosphatase were used as markers of differentiation. Interestingly, a high rate of cellular proliferation was observed at a low level of hypergravity (5 x g). Despite this, there was no difference in the time to dome formation between the control cells at primary cell density and those that differentiated under hyper- or hypogravity conditions. In conclusion, this experiment on amphibian cells revealed that the proliferation of A6 cells was strongly affected by gravity conditions, but the differentiation step appears to be controlled by an intra- or intercellular clock.
Aimar,
Microgravity and hypergravity effects on fertilization of the salamander Pleurodeles waltl (urodele amphibian).
2000, Pubmed
Aimar,
Microgravity and hypergravity effects on fertilization of the salamander Pleurodeles waltl (urodele amphibian).
2000,
Pubmed
Chen,
Epithelial sodium channel regulated by aldosterone-induced protein sgk.
1999,
Pubmed
,
Xenbase
Cogoli,
Activation and proliferation of lymphocytes and other mammalian cells in microgravity.
1997,
Pubmed
Di Sole,
Adenosine inhibits the transfected Na+-H+ exchanger NHE3 in Xenopus laevis renal epithelial cells (A6/C1).
1999,
Pubmed
,
Xenbase
Felix,
Mechanical stimulation initiates intercellular Ca2+ signaling in intact tracheal epithelium maintained under normal gravity and simulated microgravity.
1998,
Pubmed
Felix,
Selected contribution: PKC activation inhibits Ca(2+) signaling in tracheal epithelial cells kept in simulated microgravity.
2000,
Pubmed
Fotis,
Phosphorylation of the alpha-subunits of the Na+/K+-ATPase from mammalian kidneys and Xenopus oocytes by cGMP-dependent protein kinase results in stimulation of ATPase activity.
1999,
Pubmed
,
Xenbase
Furutsu,
Bidirectional effects of hypergravity on the cell growth and differentiated functions of osteoblast-like ROS17/2.8 cells.
2000,
Pubmed
Ichigi,
Dome formation and tubule morphogenesis by Xenopus kidney A6 cell cultures exposed to microgravity simulated with a 3D-clinostat and to hypergravity.
2001,
Pubmed
,
Xenbase
Kieber-Emmons,
Antiidiotypic antibody recognizes an amiloride binding domain within the alpha subunit of the epithelial Na+ channel.
1999,
Pubmed
,
Xenbase
Komazaki,
Experimental analysis of gravitational effects on amphibian gastrulation.
2002,
Pubmed
,
Xenbase
Mastroberardino,
Ras pathway activates epithelial Na+ channel and decreases its surface expression in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Mesland,
Mechanisms of gravity effects on cells: are there gravity-sensitive windows?
1992,
Pubmed
Naruse,
Involvement of stretch-activated ion channels in Ca2+ mobilization to mechanical stretch in endothelial cells.
1993,
Pubmed
Neff,
Early amphibian (anuran) morphogenesis is sensitive to novel gravitational fields.
1993,
Pubmed
,
Xenbase
Pellis,
Changes in gravity inhibit lymphocyte locomotion through type I collagen.
1997,
Pubmed
Piepenhagen,
Biogenesis of polarized epithelial cells during kidney development in situ: roles of E-cadherin-mediated cell-cell adhesion and membrane cytoskeleton organization.
1998,
Pubmed
Rafferty,
The length of secondary chromosomal constrictions in normal individuals and in a nucleolar mutant of Xenopus laevis.
1969,
Pubmed
,
Xenbase
Rehn,
Role of the cytoskeleton in stimulation of Na+ channels in A6 cells by changes in osmolality.
1998,
Pubmed
,
Xenbase
Rijken,
Effects of gravity on the cellular response to epidermal growth factor.
1994,
Pubmed
Sebastian,
Altered gravitational forces affect the development of the static vestibuloocular reflex in fish (Oreochromis mossambicus).
2001,
Pubmed
Seitzer,
Microgravity and hypergravity effects on collagen biosynthesis of human dermal fibroblasts.
1995,
Pubmed
Souza,
Amphibian development in the virtual absence of gravity.
1995,
Pubmed
,
Xenbase
Stockand,
Differential effects of protein kinase C on the levels of epithelial Na+ channel subunit proteins.
2000,
Pubmed
,
Xenbase
Ura,
Localization of Na+, K(+)-ATPase in tissues of rabbit and teleosts using an antiserum directed against a partial sequence of the alpha-subunit.
1996,
Pubmed
Urbach,
Mechanosensitive calcium entry and mobilization in renal A6 cells.
1999,
Pubmed
,
Xenbase
Verrey,
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells.
1989,
Pubmed
,
Xenbase
Zeiske,
Apical Cl- channels in A6 cells.
1998,
Pubmed
,
Xenbase
Zuckerman,
Association of the epithelial sodium channel with Apx and alpha-spectrin in A6 renal epithelial cells.
1999,
Pubmed
,
Xenbase
