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Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
Miake-Lye R, Newport J, Kirschner M.
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We have studied the effect of maturation-promoting factor (MPF) on embryonic nuclei during the early cleavage stage of Xenopus laevis development. When protein synthesis is inhibited by cycloheximide during this stage, the embryonic cell cycle arrests in an artificially produced G2 phase-like state, after completion of one additional round of DNA synthesis. Approximately 100 nuclei can be arrested in a common cytoplasm if cytokinesis is first inhibited by cytochalasin B. Within 5 min after injection of MPF into such embryos, the nuclear envelope surrounding each nucleus disperses, as determined histologically or by immunofluorescent staining of the nuclear lamina with antilamin antiserum. The breakdown of the nuclear envelope occurs at levels of MPF comparable to or slightly lower than those required for oocyte maturation. Amplification of MPF activity, however, does not occur in the arrested egg as it does in the oocyte. These results suggest that MPF can act to advance interphase nuclei into the first events of mitosis and show that the nuclear lamina responds rapidly to MPF.
Benbow,
Cytoplasmic control of nuclear DNA synthesis during early development of Xenopus laevis: a cell-free assay.
1975, Pubmed,
Xenbase
Benbow,
Cytoplasmic control of nuclear DNA synthesis during early development of Xenopus laevis: a cell-free assay.
1975,
Pubmed
,
Xenbase Burnette,
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
1981,
Pubmed Clark,
Diffusible and bound actin nuclei of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase Cohen,
The role of protein phosphorylation in neural and hormonal control of cellular activity.
1982,
Pubmed Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Eckstein,
Investigation of enzyme mechanisms with nucleoside phosphorothioates.
1975,
Pubmed Forbes,
Small nuclear RNA transcription and ribonucleoprotein assembly in early Xenopus development.
1983,
Pubmed
,
Xenbase Gerace,
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
1978,
Pubmed Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed Giloh,
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate.
1982,
Pubmed Hammer,
Cytochalasin B II: selective inhibition of cytokinesis in Xenopus laevis eggs.
1971,
Pubmed
,
Xenbase Hara,
A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
1980,
Pubmed
,
Xenbase Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase Heidemann,
Aster formation in eggs of Xenopus laevis. Induction by isolated basal bodies.
1975,
Pubmed
,
Xenbase Kishimoto,
Generality of the action of various maturation-promoting factors.
1982,
Pubmed
,
Xenbase Krohne,
Localization of a nuclear envelope-associated protein by indirect immunofluorescence microscopy using antibodies against a major polypeptide from rat liver fractions enriched in nuclear envelope-associated material.
1978,
Pubmed Lamb,
Increase in nuclear poly(A)-containing RNA at syncytial blastoderm in Drosophila melanogaster embryos.
1976,
Pubmed Landström,
Deoxynucleoside inhibition of differentiation in cultured embryonic cells.
1977,
Pubmed Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase Maller,
Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed Masui,
Oocyte maturation.
1979,
Pubmed Nelkin,
Protein factor(s) from mitotic CHO cells induce meiotic maturation in Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Pestell,
Microtubule protein synthesis during oogenesis and early embryogenesis in Xenopus laevis.
1975,
Pubmed
,
Xenbase Reynhout,
Studies on the appearance and nature of a maturation-inducing factor in the cytoplasm of amphibian oocytes exposed to progesterone.
1974,
Pubmed
,
Xenbase Smith,
Role of the oocyte nucleus in physiological maturation in Rana pipiens.
1969,
Pubmed Stick,
Immunological localization of the major architectural protein associated with the nuclear envelope of the Xenopus laevis oocyte.
1982,
Pubmed
,
Xenbase Sunkara,
Mitotic factors from mammalian cells: a preliminary characterization.
1979,
Pubmed
,
Xenbase Wasserman,
Calmodulin triggers the resumption of meiosis in amphibian oocytes.
1981,
Pubmed
,
Xenbase Wasserman,
Effects of cyclohexamide on a cytoplasmic factor initiating meiotic naturation in Xenopus oocytes.
1975,
Pubmed
,
Xenbase Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase Wu,
Partial purification and characterization of the maturation-promoting factor from eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
