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The Mos protein kinase is a key regulator of vertebrate oocyte maturation. Oocyte-specific Mos protein expression is subject to translational control. In the frog Xenopus, the translation of Mos protein requires the progesterone-induced polyadenylation of the maternal Mos mRNA, which is present in the oocytecytoplasm. Both the Xenopus p42 mitogen-activated protein kinase (MAPK) and maturation-promoting factor (MPF) signaling pathways have been proposed to mediate progesterone-stimulated oocyte maturation. In this study, we have determined the relative contributions of the MAPK and MPF signaling pathways to Mos mRNA polyadenylation. We report that progesterone-induced Mos mRNA polyadenylation was attenuated in oocytes expressing the MAPK phosphatase rVH6. Moreover, inhibition of MAPK signaling blocked progesterone-induced Mos protein accumulation. Activation of the MAPK pathway by injection of RNA encoding Mos was sufficient to induce both the polyadenylation of synthetic Mos mRNA substrates and the accumulation of endogenous Mos protein in the absence of MPF signaling. Activation of MPF, by injection of cyclin B1 RNA or purified cyclin B1 protein, also induced both Mos protein accumulation and Mos mRNA polyadenylation. However, this action of MPF required MAPK activity. By contrast, the cytoplasmic polyadenylation of maternal cyclin B1 mRNA was stimulated by MPF in a MAPK-independent manner, thus revealing a differential regulation of maternal mRNA polyadenylation by the MAPK and MPF signaling pathways. We propose that MAPK-stimulated Mos mRNA cytoplasmic polyadenylation is a key component of the positive-feedback loop, which contributes to the all-or-none process of oocyte maturation.
Abrieu,
Mitogen-activated protein kinase activation down-regulates a mechanism that inactivates cyclin B-cdc2 kinase in G2-arrested oocytes.
1997, Pubmed,
Xenbase
Abrieu,
Mitogen-activated protein kinase activation down-regulates a mechanism that inactivates cyclin B-cdc2 kinase in G2-arrested oocytes.
1997,
Pubmed
,
Xenbase
Alessi,
PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo.
1995,
Pubmed
Ballantyne,
Poly (A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic changes during oocyte maturation and early development.
1995,
Pubmed
,
Xenbase
Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase
Barkoff,
Meiotic maturation in Xenopus requires polyadenylation of multiple mRNAs.
1998,
Pubmed
,
Xenbase
Bienroth,
Purification of the cleavage and polyadenylation factor involved in the 3'-processing of messenger RNA precursors.
1991,
Pubmed
Blenis,
Signal transduction via the MAP kinases: proceed at your own RSK.
1993,
Pubmed
Brondello,
Constitutive MAP kinase phosphatase (MKP-1) expression blocks G1 specific gene transcription and S-phase entry in fibroblasts.
1995,
Pubmed
Colgan,
Cell-cycle related regulation of poly(A) polymerase by phosphorylation.
1996,
Pubmed
,
Xenbase
Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase
Cross,
PD 98059 prevents establishment of the spindle assembly checkpoint and inhibits the G2-M transition in meiotic but not mitotic cell cycles in Xenopus.
1998,
Pubmed
,
Xenbase
Daar,
A characterization of cytostatic factor activity from Xenopus eggs and c-mos-transformed cells.
1991,
Pubmed
,
Xenbase
Davis,
Transcriptional regulation by MAP kinases.
1995,
Pubmed
de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed
Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Ferrell,
The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase
Freeman,
Meiotic induction by Xenopus cyclin B is accelerated by coexpression with mosXe.
1991,
Pubmed
,
Xenbase
Freeman,
Xenopus homolog of the mos protooncogene transforms mammalian fibroblasts and induces maturation of Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Gardner,
Measuring activation of kinases in mitogen-activated protein kinase regulatory network.
1994,
Pubmed
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase
Gebauer,
Translational control by cytoplasmic polyadenylation of c-mos mRNA is necessary for oocyte maturation in the mouse.
1994,
Pubmed
Gebauer,
Cloning and characterization of a Xenopus poly(A) polymerase.
1995,
Pubmed
,
Xenbase
Gotoh,
Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
1995,
Pubmed
,
Xenbase
Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase
Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase
Haccard,
Induction of Xenopus oocyte meiotic maturation by MAP kinase.
1995,
Pubmed
,
Xenbase
Hake,
Translational regulation of maternal mRNA.
1997,
Pubmed
,
Xenbase
Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase
Huang,
Dependence of Mos-induced Cdc2 activation on MAP kinase function in a cell-free system.
1996,
Pubmed
,
Xenbase
Kanki,
Progression from meiosis I to meiosis II in Xenopus oocytes requires de novo translation of the mosxe protooncogene.
1991,
Pubmed
,
Xenbase
Keller,
Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA.
1991,
Pubmed
Kuge,
Cytoplasmic 3' poly(A) addition induces 5' cap ribose methylation: implications for translational control of maternal mRNA.
1995,
Pubmed
,
Xenbase
Kumagai,
Control of the Cdc2/cyclin B complex in Xenopus egg extracts arrested at a G2/M checkpoint with DNA synthesis inhibitors.
1995,
Pubmed
,
Xenbase
Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Macdonald,
Translational regulation of maternal mRNAs.
1996,
Pubmed
MacNicol,
Raf-1 kinase is essential for early Xenopus development and mediates the induction of mesoderm by FGF.
1993,
Pubmed
,
Xenbase
MacNicol,
pXen, a utility vector for the expression of GST-fusion proteins in Xenopus laevis oocytes and embryos.
1997,
Pubmed
,
Xenbase
MacNicol,
Regulation of Raf-1-dependent signaling during early Xenopus development.
1995,
Pubmed
,
Xenbase
Matsuda,
Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.
1992,
Pubmed
,
Xenbase
Matten,
Positive feedback between MAP kinase and Mos during Xenopus oocyte maturation.
1996,
Pubmed
,
Xenbase
McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase
McGrew,
Translational control by cytoplasmic polyadenylation during Xenopus oocyte maturation: characterization of cis and trans elements and regulation by cyclin/MPF.
1990,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Mercer,
An analysis of the rate of metallothionein mRNA poly(A)-shortening using RNA blot hybridization.
1985,
Pubmed
Mourey,
A novel cytoplasmic dual specificity protein tyrosine phosphatase implicated in muscle and neuronal differentiation.
1996,
Pubmed
Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed
,
Xenbase
Murthy,
The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation.
1995,
Pubmed
Murthy,
Characterization of the multisubunit cleavage-polyadenylation specificity factor from calf thymus.
1992,
Pubmed
Muslin,
Raf-1 protein kinase is important for progesterone-induced Xenopus oocyte maturation and acts downstream of mos.
1993,
Pubmed
,
Xenbase
Nebreda,
Newly synthesized protein(s) must associate with p34cdc2 to activate MAP kinase and MPF during progesterone-induced maturation of Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Palmer,
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
1998,
Pubmed
,
Xenbase
Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase
Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase
Pause,
Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function.
1994,
Pubmed
Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase
Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase
Qian,
Activated polo-like kinase Plx1 is required at multiple points during mitosis in Xenopus laevis.
1998,
Pubmed
,
Xenbase
Roy,
Mos proto-oncogene function during oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase
Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase
Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase
Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase
Shibuya,
Activation of the Xenopus oocyte mitogen-activated protein kinase pathway by Mos is independent of Raf.
1996,
Pubmed
,
Xenbase
Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Smith,
The induction of oocyte maturation: transmembrane signaling events and regulation of the cell cycle.
1989,
Pubmed
Stebbins-Boaz,
Multiple sequence elements and a maternal mRNA product control cdk2 RNA polyadenylation and translation during early Xenopus development.
1994,
Pubmed
,
Xenbase
Stebbins-Boaz,
Translational control during early development.
1997,
Pubmed
Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase
Sun,
MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo.
1993,
Pubmed
Thuresson,
Multiple forms of poly(A) polymerases in human cells.
1994,
Pubmed
VanRenterghem,
Reconstitution of p21ras-dependent and -independent mitogen-activated protein kinase activation in a cell-free system.
1993,
Pubmed
,
Xenbase
Vassalli,
Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
1989,
Pubmed
Watanabe,
Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.
1989,
Pubmed
,
Xenbase
