Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
A method for rapid exchange of solutions at membrane patches using a 10-microliters microcapsule.
Koh DS
,
Vogel W
.
???displayArticle.abstract???
A rapid exchange (less than 2 ms) of the bath solution facing a membrane patch is accomplished by driving the tip of a pipette from the bath through a 100-microns oil layer into a small capsule filled with 10 microliters test solution. The microcapsule method can be applied to both excised patch configurations, inside-out and outside-out patches. On and off reactions of Ca(2+)-activated K+ channel activity have been recorded after changing the intracellular Ca2+ concentration using an inside-out patch. A blockade of these K+ channels by external tetraethylammonium ions is demonstrated with an outside-out patch. The blocking kinetics of delayed-rectifier K+ channels by a purified peptide toxin from snake venom, dendrotoxin, could be measured with our microcapsule method. Using tiny volumes of test solutions this method can be helpful in experiments involving scarce or expensive solutions.
Akaike,
'Concentration-clamp' study of gamma-aminobutyric-acid-induced chloride current kinetics in frog sensory neurones.
1986, Pubmed
Akaike,
'Concentration-clamp' study of gamma-aminobutyric-acid-induced chloride current kinetics in frog sensory neurones.
1986,
Pubmed
Brett,
A method for the rapid exchange of solutions bathing excised membrane patches.
1986,
Pubmed
Bräu,
A K+ channel in Xenopus nerve fibres selectively blocked by bee and snake toxins: binding and voltage-clamp experiments.
1990,
Pubmed
,
Xenbase
Corrette,
Two types of fast K+ channels in rat myelinated nerve fibres and their sensitivity to dendrotoxin.
1991,
Pubmed
Duszyk,
The 20-pS chloride channel of the human airway epithelium.
1990,
Pubmed
Fenwick,
A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine.
1982,
Pubmed
Franciolini,
Anion and cation permeability of a chloride channel in rat hippocampal neurons.
1987,
Pubmed
Franke,
Liquid filament switch for ultra-fast exchanges of solutions at excised patches of synaptic membrane of crayfish muscle.
1987,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Jonas,
Single-channel recording in myelinated nerve fibers reveals one type of Na channel but different K channels.
1989,
Pubmed
,
Xenbase
Jonas,
ATP-sensitive and Ca-activated K channels in vertebrate axons: novel links between metabolism and excitability.
1991,
Pubmed
,
Xenbase
Magleby,
Calcium dependence of open and shut interval distributions from calcium-activated potassium channels in cultured rat muscle.
1983,
Pubmed
Markwardt,
Gating of maxi K+ channels studied by Ca2+ concentration jumps in excised inside-out multi-channel patches (myocytes from guinea pig urinary bladder).
1992,
Pubmed
Milton,
How do patch clamp seals form? A lipid bleb model.
1990,
Pubmed
Neher,
Single-channel currents recorded from membrane of denervated frog muscle fibres.
1976,
Pubmed
Qin,
A new oil-gate concentration jump technique applied to inside-out patch-clamp recording.
1988,
Pubmed
Sakai,
Anion and cation channels in the basolateral membrane of rabbit parietal cells.
1989,
Pubmed
Yellen,
Single Ca2+-activated nonselective cation channels in neuroblastoma.
1982,
Pubmed
