Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Xenopus laevis serum albumins are encoded in two closely related genes.
Westley B
,
Wyler T
,
Ryffel G
,
Weber R
.
???displayArticle.abstract???
cDNA clones containing sequences complementary to Xenopus laevis albumin mRNA have been identified in a collection of cDNA clones made from poly(A)+ RNA prepared from male Xenopus laevis liver. Although all the albumin cDNA clones crosshybridise, restriction enzyme and heteroduplex analysis show that there are 2 closely related albumin mRNA sequences. The 2 albumin mRNAs are only mismatched by 8% but could be isolated by positive selection using stringent hybridization conditions. Oocytes injected with the 2 purified mRNAs, secreted either the 68,000 or 74,000 dalton albumin into the culture medium showing that the 2 albumins of X. laevis serum are encoded in the 2 closely related mRNAs. Measurements of the abundance of albumin mRNA show that the 2 albumin mRNAs together account for about 9% of total poly(A)+ RNA in male Xenopus laevis liver but the mRNA coding for the 74,000 dalton mRNA is about twice as abundant as that coding for the 68,000 dalton mRNA.
BARTH,
Differentiation of cells of the Rana pipiens gastrula in unconditioned medium.
1959, Pubmed
BARTH,
Differentiation of cells of the Rana pipiens gastrula in unconditioned medium.
1959,
Pubmed
Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Bishop,
Three abundance classes in HeLa cell messenger RNA.
1974,
Pubmed
Bozzoni,
Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.
1981,
Pubmed
,
Xenbase
Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed
Gordon,
Cloning of a double-stranded cDNA that codes for a portion of chicken preproalbumin. A general method for isolating a specific DNA sequence from partially purified mRNA.
1978,
Pubmed
Grunstein,
Colony hybridization.
1979,
Pubmed
Jeffreys,
Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization.
1980,
Pubmed
,
Xenbase
Laird,
Rate of fixation of nucleotide substitutions in evolution.
1969,
Pubmed
Lane,
The Xenopus oocyte as a surrogate secretory system. The specificity of protein export.
1980,
Pubmed
,
Xenbase
Ledford,
Albumin synthesis during induced and spontaneous metamorphosis in the bullfrog Rana catesbeiana.
1973,
Pubmed
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Ryffel,
Comparison of cytoplasmic and nuclear poly(A)-containing RNA sequences in Xenopus liver cells.
1976,
Pubmed
,
Xenbase
Ryffel,
Quantitation of vitellogenin messenger RNA in the liver of male Xenopus toads during primary and secondary stimulation by estrogen.
1977,
Pubmed
,
Xenbase
Sargent,
The rat serum albumin gene: analysis of cloned sequences.
1979,
Pubmed
Stark,
Quantitative analysis of specific labelled RNA'S using DNA covalently linked to diazobenzyloxymethyl-paper.
1979,
Pubmed
Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed
Ullman,
The relationship between mismatched base pairs and the thermal stability of DNA duplexes. I. Effects of depurination and chain scission.
1973,
Pubmed
Wahli,
Vitellogenin in Xenopus laevis is encoded in a small family of genes.
1979,
Pubmed
,
Xenbase
Wensink,
A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
1974,
Pubmed
Westley,
A secreted glycoprotein induced by estrogen in human breast cancer cell lines.
1980,
Pubmed
Wieslander,
A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.
1979,
Pubmed
Young,
A single mouse alpha-amylase gene specifies two different tissue-specific mRNAs.
1981,
Pubmed