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Coinfection of insect cells with recombinant baculovirus expressing pp60v-src results in the activation of a serine-specific protein kinase pp90rsk.
Vik TA
,
Sweet LJ
,
Erikson RL
.
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A recombinant baculovirus was constructed for the production of the serine-specific protein kinase, pp90rsk (where rsk is ribosomal S6 kinase), in insect cells. The Xenopus pp90rsk expressed in the infected cells had nearly undetectable enzyme activity in contrast to the same enzyme coproduced with the v-src oncogene product pp60v-src. The transforming gene product pp60v-src very effectively activated pp90rsk, whereas the products of c-src and the myristoylation-minus nontransforming virus NY315 were markedly less effective. Only a fraction of the total pp90rsk population was activated, and it could be partially separated from unactivated protein by ion-exchange chromatography. When compared to the unactivated form, the activated enzyme displayed about a 4000-fold increase in the capacity to phosphorylate the ribosomal protein S6. The enhanced enzymatic activity appeared to be due to phosphorylation of pp90rsk.
Alcorta,
Sequence and expression of chicken and mouse rsk: homologs of Xenopus laevis ribosomal S6 kinase.
1989, Pubmed,
Xenbase
Alcorta,
Sequence and expression of chicken and mouse rsk: homologs of Xenopus laevis ribosomal S6 kinase.
1989,
Pubmed
,
Xenbase
Anderson,
Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
1990,
Pubmed
Ballou,
Protein phosphatase 2A inactivates the mitogen-stimulated S6 kinase from Swiss mouse 3T3 cells.
1988,
Pubmed
Ballou,
S6 kinase in quiescent Swiss mouse 3T3 cells is activated by phosphorylation in response to serum treatment.
1988,
Pubmed
Bialojan,
Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics.
1988,
Pubmed
Blenis,
Identification of a ribosomal protein S6 kinase regulated by transformation and growth-promoting stimuli.
1987,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Carroll,
Serum-stimulated cell growth causes oscillations in casein kinase II activity.
1989,
Pubmed
Cohen,
Protein phosphatases come of age.
1989,
Pubmed
Cross,
A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation.
1984,
Pubmed
Erikson,
A protein kinase from Xenopus eggs specific for ribosomal protein S6.
1985,
Pubmed
,
Xenbase
Erikson,
Purification and characterization of a protein kinase from Xenopus eggs highly specific for ribosomal protein S6.
1986,
Pubmed
,
Xenbase
Erikson,
Molecular events in cells transformed by Rous Sarcoma virus.
1980,
Pubmed
Erikson,
Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.
1987,
Pubmed
,
Xenbase
Erikson,
In vivo phosphorylation and activation of ribosomal protein S6 kinases during Xenopus oocyte maturation.
1989,
Pubmed
,
Xenbase
Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
,
Xenbase
Gilmer,
Construction of plasmids for expression of Rous sarcoma virus transforming protein, p60src, in Escherichia coli.
1982,
Pubmed
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Hunter,
Protein-tyrosine kinases.
1985,
Pubmed
Hunter,
A tail of two src's: mutatis mutandis.
1987,
Pubmed
Jones,
A Xenopus ribosomal protein S6 kinase has two apparent kinase domains that are each similar to distinct protein kinases.
1988,
Pubmed
,
Xenbase
Kmiecik,
Regulation by the autophosphorylation site in overexpressed pp60c-src.
1988,
Pubmed
Krebs,
Casein kinase II as a potentially important enzyme concerned with signal transduction.
1988,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Luckow,
High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors.
1989,
Pubmed
Morrison,
Direct activation of the serine/threonine kinase activity of Raf-1 through tyrosine phosphorylation by the PDGF beta-receptor.
1989,
Pubmed
Morrison,
Signal transduction from membrane to cytoplasm: growth factors and membrane-bound oncogene products increase Raf-1 phosphorylation and associated protein kinase activity.
1988,
Pubmed
Piwnica-Worms,
Regulation of pp60c-src and its interaction with polyomavirus middle T antigen in insect cells.
1990,
Pubmed
Ray,
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
1987,
Pubmed
Ray,
Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.
1988,
Pubmed
Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase
Tabarini,
Purification of a bovine liver S6 kinase.
1987,
Pubmed
Ushiro,
Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
1980,
Pubmed
Wool,
The structure and function of eukaryotic ribosomes.
1979,
Pubmed
