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J Biol Chem
2009 Jan 09;2842:1064-74. doi: 10.1074/jbc.M807273200.
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Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
Shechter D
,
Nicklay JJ
,
Chitta RK
,
Shabanowitz J
,
Hunt DF
,
Allis CD
.
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Histone proteins contain epigenetic information that is encoded both in the relative abundance of core histones and variants and particularly in the post-translational modification of these proteins. We determined the presence of such variants and covalent modifications in seven tissue types of the anuran Xenopus laevis, including oocyte, egg, sperm, early embryo equivalent (pronuclei incubated in egg extract), S3 neurula cells, A6 kidney cells, and erythrocytes. We first developed a new robust method for isolating the stored, predeposition histones from oocytes and eggs via chromatography on heparin-Sepharose, whereas we isolated chromatinized histones via conventional acid extraction. We identified two previously unknown H1 isoforms (H1fx and H1B.Sp) present on sperm chromatin. We immunoblotted this global collection of histones with many specific post-translational modification antibodies, including antibodies against methylated histone H3 on Lys(4), Lys(9), Lys(27), Lys(79), Arg(2), Arg(17), and Arg(26); methylated histone H4 on Lys(20); methylated H2A and H4 on Arg(3); acetylated H4 on Lys(5), Lys(8), Lys(12), and Lys(16) and H3 on Lys(9) and Lys(14); and phosphorylated H3 on Ser(10) and H2A/H4 on Ser(1). Furthermore, we subjected a subset of these histones to two-dimensional gel analysis and subsequent immunoblotting and mass spectrometry to determine the global remodeling of histone modifications that occurs as development proceeds. Overall, our observations suggest that each metazoan cell type may have a unique histone modification signature correlated with its differentiation status.
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974, Pubmed,
Xenbase
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974,
Pubmed
,
Xenbase
Adamson,
Changes in the rate of histone synthesis during oocyte maturation and very early development of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Allis,
Deposition-related histone acetylation in micronuclei of conjugating Tetrahymena.
1985,
Pubmed
Almouzni,
Competition between transcription complex assembly and chromatin assembly on replicating DNA.
1990,
Pubmed
,
Xenbase
Barber,
The enhancement of histone H4 and H2A serine 1 phosphorylation during mitosis and S-phase is evolutionarily conserved.
2004,
Pubmed
,
Xenbase
Barski,
High-resolution profiling of histone methylations in the human genome.
2007,
Pubmed
Bedford,
Arginine methylation an emerging regulator of protein function.
2005,
Pubmed
Berger,
The complex language of chromatin regulation during transcription.
2007,
Pubmed
Bernstein,
The nucleosome: a little variation goes a long way.
2006,
Pubmed
Bernstein,
A bivalent chromatin structure marks key developmental genes in embryonic stem cells.
2006,
Pubmed
Czermin,
The sounds of silence--histone deacetylation meets histone methylation.
2003,
Pubmed
Dilworth,
Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts.
1987,
Pubmed
,
Xenbase
Dimitrov,
Remodeling somatic nuclei in Xenopus laevis egg extracts: molecular mechanisms for the selective release of histones H1 and H1(0) from chromatin and the acquisition of transcriptional competence.
1996,
Pubmed
,
Xenbase
Frehlick,
Characterization of the PL-I-related SP2 protein from Xenopus.
2007,
Pubmed
,
Xenbase
Funabiki,
The Xenopus chromokinesin Xkid is essential for metaphase chromosome alignment and must be degraded to allow anaphase chromosome movement.
2000,
Pubmed
,
Xenbase
Garcia,
Characterization of histones and their post-translational modifications by mass spectrometry.
2007,
Pubmed
Gilbert,
Formation of facultative heterochromatin in the absence of HP1.
2003,
Pubmed
,
Xenbase
Happel,
Characterisation of human histone H1x.
2005,
Pubmed
Huen,
Direct interaction between SET8 and proliferating cell nuclear antigen couples H4-K20 methylation with DNA replication.
2008,
Pubmed
Humphries,
Chromatin structure of the 5S ribonucleic acid genes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Kleinschmidt,
Nucleosome assembly in vitro: separate histone transfer and synergistic interaction of native histone complexes purified from nuclei of Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Lemaitre,
Mitotic remodeling of the replicon and chromosome structure.
2005,
Pubmed
,
Xenbase
Lin,
Antibodies specific to acetylated histones document the existence of deposition- and transcription-related histone acetylation in Tetrahymena.
1989,
Pubmed
Loyola,
PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state.
2006,
Pubmed
Malik,
Phylogenomics of the nucleosome.
2003,
Pubmed
Maller,
Cell cycle transitions in early Xenopus development.
2001,
Pubmed
,
Xenbase
Meneghini,
Conserved histone variant H2A.Z protects euchromatin from the ectopic spread of silent heterochromatin.
2003,
Pubmed
Nicklay,
Analysis of histones in Xenopus laevis. II. mass spectrometry reveals an index of cell type-specific modifications on H3 and H4.
2009,
Pubmed
,
Xenbase
Ruderman,
Modulations of histone messenger RNA during the early development of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Ruthenburg,
Multivalent engagement of chromatin modifications by linked binding modules.
2007,
Pubmed
Schmiedeke,
Histones have high affinity for the glomerular basement membrane. Relevance for immune complex formation in lupus nephritis.
1989,
Pubmed
Schmitt,
Histone H3 phosphorylation during Xenopus oocyte maturation: regulation by the MAP kinase/p90Rsk pathway and uncoupling from DNA condensation.
2002,
Pubmed
,
Xenbase
Shechter,
ATR and ATM regulate the timing of DNA replication origin firing.
2004,
Pubmed
,
Xenbase
Shechter,
Extraction, purification and analysis of histones.
2007,
Pubmed
,
Xenbase
Shevchenko,
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
1996,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Taverna,
How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
2007,
Pubmed
Turner,
Specific antibodies reveal ordered and cell-cycle-related use of histone-H4 acetylation sites in mammalian cells.
1989,
Pubmed
Wang,
Human PAD4 regulates histone arginine methylation levels via demethylimination.
2004,
Pubmed
Woodland,
Histone synthesis during the development of Xenopus.
1980,
Pubmed
,
Xenbase
Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Woodland,
The modification of stored histones H3 and H4 during the oogenesis and early development of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Wysocka,
Histone arginine methylation and its dynamic regulation.
2006,
Pubmed
Zucker,
The histone H3/H4.N1 complex supplemented with histone H2A-H2B dimers and DNA topoisomerase I forms nucleosomes on circular DNA under physiological conditions.
1990,
Pubmed
,
Xenbase
