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To understand how microtubules are generated in the growth cone, we have imaged fluorescently tagged microtubules in living frog embryonic neurons. The neurons were labeled by injecting rhodamine-labeled tubulin into the fertilized egg and explanting the neurons from the neural tube. Microtubules extend deep into the growth cone periphery and adopt three characteristic distributions: (a) dispersed and splayed throughout much of the growth cone; (b) looped and apparently contorted by compression; and (c) bundled into tight arrays. These distributions interconvert on a time scale of several minutes and these interconversions are correlated with the behavior of the growth cone. We observed microtubule growth and shrinkage in growth cones, but are unable to determine their contribution to net assembly. However, translocation of polymer form the axon appears to be a major mechanism of generating new polymer in the growth cone, while bundling of microtubules in the growth cone appears to be the critical step in generating new axon. Neurons that were about to turn spontaneously generated microtubules in the future direction of growth, suggesting that orientation of microtubules might be an important early step in neuronal pathfinding.
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990, Pubmed,
Xenbase
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Bentley,
Disoriented pathfinding by pioneer neurone growth cones deprived of filopodia by cytochalasin treatment.
,
Pubmed
Bray,
Growth cone formation in cultures of sensory neurons.
1978,
Pubmed
Bridgman,
The organization of myosin and actin in rapid frozen nerve growth cones.
1989,
Pubmed
Bunge,
Fine structure of nerve fibers and growth cones of isolated sympathetic neurons in culture.
1973,
Pubmed
Cassimeris,
Real-time observations of microtubule dynamic instability in living cells.
1988,
Pubmed
Cheng,
Polarized compartmentalization of organelles in growth cones from developing optic tectum.
1985,
Pubmed
Daniels,
Colchicine inhibition of nerve fiber formation in vitro.
1972,
Pubmed
Forscher,
Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone.
1988,
Pubmed
Gard,
A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end.
1987,
Pubmed
,
Xenbase
Goldberg,
Stages in axon formation: observations of growth of Aplysia axons in culture using video-enhanced contrast-differential interference contrast microscopy.
1986,
Pubmed
Harris,
Growth cones of developing retinal cells in vivo, on culture surfaces, and in collagen matrices.
1985,
Pubmed
,
Xenbase
Heidemann,
Polarity orientation of axonal microtubules.
1981,
Pubmed
Hinkle,
The direction of growth of differentiating neurones and myoblasts from frog embryos in an applied electric field.
1981,
Pubmed
,
Xenbase
Hyman,
Preparation of modified tubulins.
1991,
Pubmed
Lankford,
Ultrastructure of individual neurons isolated from avian retina: occurrence of microtubule loops in dendrites.
1990,
Pubmed
Marsh,
Growth of neurites without filopodial or lamellipodial activity in the presence of cytochalasin B.
1984,
Pubmed
Mitchison,
Cytoskeletal dynamics and nerve growth.
1988,
Pubmed
Mitchison,
Some thoughts on the partitioning of tubulin between monomer and polymer under conditions of dynamic instability.
1987,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Reinsch,
Microtubule polymer assembly and transport during axonal elongation.
1991,
Pubmed
,
Xenbase
Sabry,
Microtubule behavior during guidance of pioneer neuron growth cones in situ.
1991,
Pubmed
Sammak,
Direct observation of microtubule dynamics in living cells.
1988,
Pubmed
Schnapp,
Cytoplasmic structure in rapid-frozen axons.
1982,
Pubmed
Schulze,
New features of microtubule behaviour observed in vivo.
1988,
Pubmed
Tsui,
Novel organization of microtubules in cultured central nervous system neurons: formation of hairpin loops at ends of maturing neurites.
1984,
Pubmed
Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed
Yamada,
Ultrastructure and function of growth cones and axons of cultured nerve cells.
1971,
Pubmed