XB-ART-21751
Cell Motil Cytoskeleton
1994 Jan 01;282:108-18. doi: 10.1002/cm.970280203.
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Purification of microtubule proteins from Xenopus egg extracts: identification of a 230K MAP4-like protein.
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We describe the purification of microtubule proteins from Xenopus egg extracts by temperature-dependent assembly and disassembly in the presence of dimethyl sulfoxide and identify a number of presumptive microtubule-associated proteins (MAPs). One of these proteins has a molecular weight of 230 kDa and is immunologically related to HeLa MAP4. We show that this MAP is heat stable and phosphorylated, and that it promotes elongation of microtubules from axonemes.
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Species referenced: Xenopus
Genes referenced: map4