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???displayArticle.abstract??? Cdc25, the dual-specificity phosphatase that dephosphorylates the Cdc2-cyclin B complex at mitosis, is highly regulated during the cell cycle. In Xenopus egg extracts, Cdc25 is associated with two isoforms of the 14-3-3 protein. Cdc25 is complexed primarily with 14-3-3epsilon and to a lesser extent with 14-3-3zeta. The association of these 14-3-3 proteins with Cdc25 varies dramatically during the cell cycle: binding is high during interphase but virtually absent at mitosis. Interaction with 14-3-3 is mediated by phosphorylation of Xenopus Cdc25 at Ser-287, which resides in a consensus 14-3-3 binding site. Recombinant Cdc25 with a point mutation at this residue (Cdc25-S287A) is incapable of binding to 14-3-3. Addition of the Cdc25-S287A mutant to Xenopus egg extracts accelerates mitosis and overrides checkpoint-mediated arrests of mitotic entry due to the presence of unreplicated and damaged DNA. These findings indicate that 14-3-3 proteins act as negative regulators of Cdc25 in controlling the G2-M transition.
Aitken,
14-3-3 and its possible role in co-ordinating multiple signalling pathways.
1996, Pubmed
Aitken,
14-3-3 and its possible role in co-ordinating multiple signalling pathways.
1996,
Pubmed al-Khodairy,
DNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe.
1992,
Pubmed Blasina,
The role of inhibitory phosphorylation of CDC2 following DNA replication block and radiation-induced damage in human cells.
1997,
Pubmed Boyle,
Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates.
1991,
Pubmed Carpenter,
Role for a Xenopus Orc2-related protein in controlling DNA replication.
1996,
Pubmed
,
Xenbase Clarke,
Dephosphorylation of cdc25-C by a type-2A protein phosphatase: specific regulation during the cell cycle in Xenopus egg extracts.
1993,
Pubmed
,
Xenbase Coleman,
Cdc2 regulatory factors.
1994,
Pubmed Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase Conklin,
14-3-3 proteins associate with cdc25 phosphatases.
1995,
Pubmed Dasso,
Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: studies in Xenopus.
1990,
Pubmed
,
Xenbase Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed Elledge,
Cell cycle checkpoints: preventing an identity crisis.
1996,
Pubmed Enoch,
Mutation of fission yeast cell cycle control genes abolishes dependence of mitosis on DNA replication.
1990,
Pubmed Featherstone,
Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase.
1991,
Pubmed Ford,
14-3-3 protein homologs required for the DNA damage checkpoint in fission yeast.
1994,
Pubmed Furnari,
Cdc25 mitotic inducer targeted by chk1 DNA damage checkpoint kinase.
1997,
Pubmed Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase Girard,
cdc25 is a nuclear protein expressed constitutively throughout the cell cycle in nontransformed mammalian cells.
1992,
Pubmed Hellman,
Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing.
1995,
Pubmed Hoffmann,
Phosphorylation and activation of human cdc25-C by cdc2--cyclin B and its involvement in the self-amplification of MPF at mitosis.
1993,
Pubmed
,
Xenbase Igarashi,
Wee1(+)-like gene in human cells.
1991,
Pubmed Izumi,
Phosphorylation and activation of the Xenopus Cdc25 phosphatase in the absence of Cdc2 and Cdk2 kinase activity.
1995,
Pubmed
,
Xenbase Izumi,
Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.
1992,
Pubmed
,
Xenbase Jin,
Role of inhibitory CDC2 phosphorylation in radiation-induced G2 arrest in human cells.
1996,
Pubmed King,
Mitosis in transition.
1994,
Pubmed Kousteni,
Sequence and expression analysis of a Xenopus laevis cDNA which encodes a homologue of mammalian 14-3-3 zeta protein.
1997,
Pubmed
,
Xenbase Kumagai,
Control of the Cdc2/cyclin B complex in Xenopus egg extracts arrested at a G2/M checkpoint with DNA synthesis inhibitors.
1995,
Pubmed
,
Xenbase Kumagai,
Regulation of Xenopus Cdc25 protein.
1997,
Pubmed
,
Xenbase Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Kumagai,
Regulation of the cdc25 protein during the cell cycle in Xenopus extracts.
1992,
Pubmed
,
Xenbase Lundgren,
mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2.
1991,
Pubmed McGowan,
Cell cycle regulation of human WEE1.
1995,
Pubmed Millar,
p55CDC25 is a nuclear protein required for the initiation of mitosis in human cells.
1991,
Pubmed Morgan,
Principles of CDK regulation.
1995,
Pubmed Mueller,
Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15.
1995,
Pubmed
,
Xenbase Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed
,
Xenbase Murray,
Cell cycle extracts.
1991,
Pubmed Muslin,
Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine.
1996,
Pubmed
,
Xenbase O'Connell,
Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation.
1997,
Pubmed Ogg,
Purification of a serine kinase that associates with and phosphorylates human Cdc25C on serine 216.
1994,
Pubmed Parker,
Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase.
1992,
Pubmed Peng,
Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.
1997,
Pubmed Rhind,
Cdc2 tyrosine phosphorylation is required for the DNA damage checkpoint in fission yeast.
1997,
Pubmed Sanchez,
Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25.
1997,
Pubmed Seki,
Chromosome condensation caused by loss of RCC1 function requires the cdc25C protein that is located in the cytoplasm.
1992,
Pubmed Smythe,
Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts.
1991,
Pubmed
,
Xenbase Strausfeld,
Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein.
1991,
Pubmed Watanabe,
Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle.
1995,
Pubmed
