Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2006 Aug 09;2515:3664-74. doi: 10.1038/sj.emboj.7601238.
Show Gene links
Show Anatomy links
Xenopus laevis POU91 protein, an Oct3/4 homologue, regulates competence transitions from mesoderm to neural cell fates.
Snir M
,
Ofir R
,
Elias S
,
Frank D
.
???displayArticle.abstract???
Cellular competence is defined as a cell's ability to respond to signaling cues as a function of time. In Xenopus laevis, cellular responsiveness to fibroblast growth factor (FGF) changes during development. At blastula stages, FGF induces mesoderm, but at gastrula stages FGF regulates neuroectoderm formation. A Xenopus Oct3/4 homologue gene, XLPOU91, regulates mesoderm to neuroectoderm transitions. Ectopic XLPOU91 expression in Xenopus embryos inhibits FGF induction of Brachyury (Xbra), eliminating mesoderm, whereas neural induction is unaffected. XLPOU91 knockdown induces high levels of Xbra expression, with blastopore closure being delayed to later neurula stages. In morphant ectoderm explants, mesoderm responsiveness to FGF is extended from blastula to gastrula stages. The initial expression of mesoderm and endoderm markers is normal, but neural induction is abolished. Churchill (chch) and Sip1, two genes regulating neural competence, are not expressed in XLPOU91 morphant embryos. Ectopic Sip1 or chch expression rescues the morphant phenotype. Thus, XLPOU91 epistatically lies upstream of chch/Sip1 gene expression, regulating the competence transition that is critical for neural induction. In the absence of XLPOU91 activity, the cues driving proper embryonic cell fates are lost.
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991, Pubmed,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Baker,
Wnt signaling in Xenopus embryos inhibits bmp4 expression and activates neural development.
1999,
Pubmed
,
Xenbase
Bonstein,
Paraxial-fated mesoderm is required for neural crest induction in Xenopus embryos.
1998,
Pubmed
,
Xenbase
Burgess,
The zebrafish spiel-ohne-grenzen (spg) gene encodes the POU domain protein Pou2 related to mammalian Oct4 and is essential for formation of the midbrain and hindbrain, and for pre-gastrula morphogenesis.
2002,
Pubmed
Cao,
The POU factor Oct-25 regulates the Xvent-2B gene and counteracts terminal differentiation in Xenopus embryos.
2004,
Pubmed
,
Xenbase
Chang,
A post-mid-blastula transition requirement for TGFbeta signaling in early endodermal specification.
2000,
Pubmed
,
Xenbase
Conlon,
Inhibition of Xbra transcription activation causes defects in mesodermal patterning and reveals autoregulation of Xbra in dorsal mesoderm.
1996,
Pubmed
,
Xenbase
Cox,
Caudalization of neural fate by tissue recombination and bFGF.
1995,
Pubmed
,
Xenbase
Cunliffe,
Ectopic mesoderm formation in Xenopus embryos caused by widespread expression of a Brachyury homologue.
1992,
Pubmed
,
Xenbase
Dibner,
XMeis3 protein activity is required for proper hindbrain patterning in Xenopus laevis embryos.
2001,
Pubmed
,
Xenbase
Eisaki,
XSIP1, a member of two-handed zinc finger proteins, induced anterior neural markers in Xenopus laevis animal cap.
2000,
Pubmed
,
Xenbase
Frank,
Localized expression of a Xenopus POU gene depends on cell-autonomous transcriptional activation and induction-dependent inactivation.
1992,
Pubmed
,
Xenbase
Gawantka,
Antagonizing the Spemann organizer: role of the homeobox gene Xvent-1.
1995,
Pubmed
,
Xenbase
Green,
The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF.
1990,
Pubmed
,
Xenbase
Harland,
Formation and function of Spemann's organizer.
1997,
Pubmed
Hauptmann,
spiel ohne grenzen/pou2 is required for zebrafish hindbrain segmentation.
2002,
Pubmed
,
Xenbase
Hemmati-Brivanlou,
Inhibition of activin receptor signaling promotes neuralization in Xenopus.
1994,
Pubmed
,
Xenbase
Henig,
A POU protein regulates mesodermal competence to FGF in Xenopus.
1998,
Pubmed
,
Xenbase
Higashi,
Generation of the floxed allele of the SIP1 (Smad-interacting protein 1) gene for Cre-mediated conditional knockout in the mouse.
2002,
Pubmed
Hinkley,
Sequential expression of multiple POU proteins during amphibian early development.
1992,
Pubmed
,
Xenbase
Isaacs,
eFGF regulates Xbra expression during Xenopus gastrulation.
1994,
Pubmed
,
Xenbase
Kato,
Neuralization of the Xenopus embryo by inhibition of p300/ CREB-binding protein function.
1999,
Pubmed
,
Xenbase
Khokha,
Depletion of three BMP antagonists from Spemann's organizer leads to a catastrophic loss of dorsal structures.
2005,
Pubmed
,
Xenbase
Kimelman,
Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
LaBonne,
Role of MAP kinase in mesoderm induction and axial patterning during Xenopus development.
1995,
Pubmed
,
Xenbase
Lamb,
Fibroblast growth factor is a direct neural inducer, which combined with noggin generates anterior-posterior neural pattern.
1995,
Pubmed
,
Xenbase
Lunde,
Zebrafish pou5f1/pou2, homolog of mammalian Oct4, functions in the endoderm specification cascade.
2004,
Pubmed
Nichols,
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
1998,
Pubmed
Nicolas,
Expression of myogenic regulatory factors during muscle development of Xenopus: myogenin mRNA accumulation is limited strictly to secondary myogenesis.
1998,
Pubmed
,
Xenbase
Nitta,
XSIP1 is essential for early neural gene expression and neural differentiation by suppression of BMP signaling.
2004,
Pubmed
,
Xenbase
Niwa,
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.
2000,
Pubmed
Papin,
Dynamic regulation of Brachyury expression in the amphibian embryo by XSIP1.
2002,
Pubmed
,
Xenbase
Postigo,
Opposing functions of ZEB proteins in the regulation of the TGFbeta/BMP signaling pathway.
2003,
Pubmed
Re'em-Kalma,
Competition between noggin and bone morphogenetic protein 4 activities may regulate dorsalization during Xenopus development.
1995,
Pubmed
,
Xenbase
Reim,
Spiel-ohne-grenzen/pou2 mediates regional competence to respond to Fgf8 during zebrafish early neural development.
2002,
Pubmed
Reim,
The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova.
2004,
Pubmed
Ribisi,
Ras-mediated FGF signaling is required for the formation of posterior but not anterior neural tissue in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Rosner,
A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo.
1990,
Pubmed
Sasai,
Endoderm induction by the organizer-secreted factors chordin and noggin in Xenopus animal caps.
1996,
Pubmed
,
Xenbase
Schöler,
New type of POU domain in germ line-specific protein Oct-4.
1990,
Pubmed
Sheng,
Churchill, a zinc finger transcriptional activator, regulates the transition between gastrulation and neurulation.
2003,
Pubmed
,
Xenbase
Shimozaki,
Involvement of Oct3/4 in the enhancement of neuronal differentiation of ES cells in neurogenesis-inducing cultures.
2003,
Pubmed
Streit,
Initiation of neural induction by FGF signalling before gastrulation.
2000,
Pubmed
Streit,
Chordin regulates primitive streak development and the stability of induced neural cells, but is not sufficient for neural induction in the chick embryo.
1998,
Pubmed
Takahashi,
Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center.
2000,
Pubmed
,
Xenbase
Umbhauer,
Mesoderm induction in Xenopus caused by activation of MAP kinase.
1995,
Pubmed
,
Xenbase
Van de Putte,
Mice lacking ZFHX1B, the gene that codes for Smad-interacting protein-1, reveal a role for multiple neural crest cell defects in the etiology of Hirschsprung disease-mental retardation syndrome.
2003,
Pubmed
Verschueren,
SIP1, a novel zinc finger/homeodomain repressor, interacts with Smad proteins and binds to 5'-CACCT sequences in candidate target genes.
1999,
Pubmed
,
Xenbase
Wilson,
An early requirement for FGF signalling in the acquisition of neural cell fate in the chick embryo.
2000,
Pubmed
,
Xenbase
Wilson,
Mesodermal patterning by an inducer gradient depends on secondary cell-cell communication.
1994,
Pubmed
,
Xenbase
Yang,
Beta-catenin/Tcf-regulated transcription prior to the midblastula transition.
2002,
Pubmed
,
Xenbase
van Grunsven,
XSIP1, a Xenopus zinc finger/homeodomain encoding gene highly expressed during early neural development.
2000,
Pubmed
,
Xenbase
