Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Neurosci
2007 Mar 07;2710:2444-56. doi: 10.1523/JNEUROSCI.4434-06.2007.
Show Gene links
Show Anatomy links
Cell-autonomous TrkB signaling in presynaptic retinal ganglion cells mediates axon arbor growth and synapse maturation during the establishment of retinotectal synaptic connectivity.
Marshak S
,
Nikolakopoulou AM
,
Dirks R
,
Martens GJ
,
Cohen-Cory S
.
???displayArticle.abstract??? BDNF contributes to the activity-dependent establishment and refinement of visual connectivity. In Xenopus, BDNF applications in the optic tectum influence retinal ganglion cell (RGC) axon branching and promote synapse formation and stabilization. The expression patterns of BDNF and TrkB suggest that BDNF specifically regulates the maturation of RGC axons at the target. It is possible, however, that BDNF modulates retinotectal synaptic connectivity by differentially influencing presynaptic RGC axons and postsynaptic tectal cells. Here, we combined single-cell expression of a dominant-negative TrkB-enhanced green fluorescent protein (GFP) fusion protein with confocal microscopy imaging in live Xenopus tadpoles to differentiate between presynaptic and postsynaptic actions of BDNF. Disruption of TrkB signaling in individual RGCs influenced the branching and synaptic maturation of presynaptic axon arbors. Specifically, GFP-TrkB.T1 overexpression increased the proportion of axons with immature, growth cone-like morphology, decreased axon branch stability, and increased axon arbor degeneration. In addition, GFP-TrkB.T1 overexpression reduced the number of red fluorescent protein-synaptobrevin-labeled presynaptic specializations per axon terminal. In contrast, overexpression of GFP-TrkB.T1 in tectal neurons did not alter synaptic number or the morphology or dynamic behavior of their dendritic arbors. Electron microscopy analysis revealed a significant decrease in the number of mature synaptic profiles and in the number of docked synaptic vesicles at retinotectal synapses made by RGC axons expressing GFP-TrkB.T1. Together, our results demonstrate that presynaptic TrkB signaling in RGCs is a key determinant in the establishment of visual connectivity and indicate that changes in tectal neuron synaptic connectivity are secondary to the BDNF-elicited enhanced stability and growth of presynaptic RGCs.
Alsina,
Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF.
2001, Pubmed,
Xenbase
Alsina,
Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF.
2001,
Pubmed
,
Xenbase
Amaya,
A method for generating transgenic frog embryos.
1999,
Pubmed
,
Xenbase
Berardi,
Molecular basis of plasticity in the visual cortex.
2003,
Pubmed
Biffo,
Selective binding and internalisation by truncated receptors restrict the availability of BDNF during development.
1995,
Pubmed
Butowt,
Anterograde axonal transport of BDNF and NT-3 by retinal ganglion cells: roles of neurotrophin receptors.
2005,
Pubmed
Campbell,
A monomeric red fluorescent protein.
2002,
Pubmed
Carter,
Brain-derived neurotrophic factor modulates cerebellar plasticity and synaptic ultrastructure.
2002,
Pubmed
Chakravarthy,
Postsynaptic TrkB signaling has distinct roles in spine maintenance in adult visual cortex and hippocampus.
2006,
Pubmed
Chen,
Cdc42 participates in the regulation of ADF/cofilin and retinal growth cone filopodia by brain derived neurotrophic factor.
2006,
Pubmed
Cohen-Cory,
Neurotrophic regulation of retinal ganglion cell synaptic connectivity: from axons and dendrites to synapses.
2004,
Pubmed
,
Xenbase
Cohen-Cory,
Effects of brain-derived neurotrophic factor on optic axon branching and remodelling in vivo.
1995,
Pubmed
,
Xenbase
Cohen-Cory,
BDNF in the development of the visual system of Xenopus.
1994,
Pubmed
,
Xenbase
Cohen-Cory,
The cellular patterns of BDNF and trkB expression suggest multiple roles for BDNF during Xenopus visual system development.
1996,
Pubmed
,
Xenbase
Collin,
The coding sequence of amyloid-beta precursor protein APP contains a neural-specific promoter element.
2006,
Pubmed
,
Xenbase
Du,
Rapid BDNF-induced retrograde synaptic modification in a developing retinotectal system.
2004,
Pubmed
,
Xenbase
Eide,
Naturally occurring truncated trkB receptors have dominant inhibitory effects on brain-derived neurotrophic factor signaling.
1996,
Pubmed
,
Xenbase
Elmariah,
Postsynaptic TrkB-mediated signaling modulates excitatory and inhibitory neurotransmitter receptor clustering at hippocampal synapses.
2004,
Pubmed
Elmariah,
Astrocytes regulate inhibitory synapse formation via Trk-mediated modulation of postsynaptic GABAA receptors.
2005,
Pubmed
Garner,
Expression of TrkB receptor isoforms in the developing avian visual system.
1996,
Pubmed
Gehler,
Brain-derived neurotrophic factor regulation of retinal growth cone filopodial dynamics is mediated through actin depolymerizing factor/cofilin.
2004,
Pubmed
Gibney,
Cytoskeletal dynamics underlying collateral membrane protrusions induced by neurotrophins in cultured Xenopus embryonic neurons.
2003,
Pubmed
,
Xenbase
Gonzalez,
Disruption of Trkb-mediated signaling induces disassembly of postsynaptic receptor clusters at neuromuscular junctions.
1999,
Pubmed
Haapasalo,
Truncated trkB.T1 is dominant negative inhibitor of trkB.TK+-mediated cell survival.
2001,
Pubmed
Hallböök,
Expression of neurotrophins and trk receptors in the avian retina.
1996,
Pubmed
Hanover,
Brain-derived neurotrophic factor overexpression induces precocious critical period in mouse visual cortex.
1999,
Pubmed
Herzog,
Contributions of the optic tectum and the retina as sources of brain-derived neurotrophic factor for retinal ganglion cells in the chick embryo.
1998,
Pubmed
Horch,
Destabilization of cortical dendrites and spines by BDNF.
1999,
Pubmed
Horch,
BDNF release from single cells elicits local dendritic growth in nearby neurons.
2002,
Pubmed
Hu,
BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo.
2005,
Pubmed
,
Xenbase
Huang,
BDNF regulates the maturation of inhibition and the critical period of plasticity in mouse visual cortex.
1999,
Pubmed
Isenmann,
Excess target-derived brain-derived neurotrophic factor preserves the transient uncrossed retinal projection to the superior colliculus.
1999,
Pubmed
Jin,
Brain-derived neurotrophic factor mediates activity-dependent dendritic growth in nonpyramidal neocortical interneurons in developing organotypic cultures.
2003,
Pubmed
Kaplan,
Neurotrophin signal transduction in the nervous system.
2000,
Pubmed
Kohara,
Inhibitory but not excitatory cortical neurons require presynaptic brain-derived neurotrophic factor for dendritic development, as revealed by chimera cell culture.
2003,
Pubmed
Kohn,
Functionally antagonistic interactions between the TrkA and p75 neurotrophin receptors regulate sympathetic neuron growth and target innervation.
1999,
Pubmed
Lein,
Rapid regulation of brain-derived neurotrophic factor mRNA within eye-specific circuits during ocular dominance column formation.
2000,
Pubmed
Li,
Expression of a dominant negative TrkB receptor, T1, reveals a requirement for presynaptic signaling in BDNF-induced synaptic potentiation in cultured hippocampal neurons.
1998,
Pubmed
Lin,
Mechanisms of synaptic vesicle exocytosis.
2000,
Pubmed
Llamosas,
Neurotrophin receptors expression in the developing mouse retina: an immunohistochemical study.
1997,
Pubmed
Lom,
Brain-derived neurotrophic factor differentially regulates retinal ganglion cell dendritic and axonal arborization in vivo.
1999,
Pubmed
,
Xenbase
Luikart,
TrkB has a cell-autonomous role in the establishment of hippocampal Schaffer collateral synapses.
2005,
Pubmed
Lyckman,
Normal eye-specific patterning of retinal inputs to murine subcortical visual nuclei in the absence of brain-derived neurotrophic factor.
2005,
Pubmed
Ma,
BDNF injected into the superior colliculus reduces developmental retinal ganglion cell death.
1998,
Pubmed
Magby,
Single-cell characterization of retrograde signaling by brain-derived neurotrophic factor.
2006,
Pubmed
Mandolesi,
A role for retinal brain-derived neurotrophic factor in ocular dominance plasticity.
2005,
Pubmed
Martin,
Five Trk receptors in the zebrafish.
1995,
Pubmed
Martínez,
TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections.
1998,
Pubmed
Menna,
The anterogradely transported BDNF promotes retinal axon remodeling during eye specific segregation within the LGN.
2003,
Pubmed
Meyer-Franke,
Characterization of the signaling interactions that promote the survival and growth of developing retinal ganglion cells in culture.
1995,
Pubmed
Ming,
Electrical activity modulates growth cone guidance by diffusible factors.
2001,
Pubmed
,
Xenbase
Mohun,
Upstream sequences required for tissue-specific activation of the cardiac actin gene in Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase
Ninkina,
Expression and function of TrkB variants in developing sensory neurons.
1996,
Pubmed
Offenhäuser,
BDNF binding to truncated trkB.T1 does not affect gene expression.
2002,
Pubmed
Ohba,
BDNF locally potentiates GABAergic presynaptic machineries: target-selective circuit inhibition.
2005,
Pubmed
Otal,
Lack of TrkB and TrkC signaling alters the synaptogenesis and maturation of mossy fiber terminals in the hippocampus.
2005,
Pubmed
Pollock,
TrkB receptor signaling regulates developmental death dynamics, but not final number, of retinal ganglion cells.
2003,
Pubmed
Pozzo-Miller,
Impairments in high-frequency transmission, synaptic vesicle docking, and synaptic protein distribution in the hippocampus of BDNF knockout mice.
1999,
Pubmed
Rico,
TrkB receptor signaling is required for establishment of GABAergic synapses in the cerebellum.
2002,
Pubmed
Rodriguez-Tébar,
The survival of chick retinal ganglion cells in response to brain-derived neurotrophic factor depends on their embryonic age.
1989,
Pubmed
Rohrer,
Neurotrophin receptor TrkB activation is not required for the postnatal survival of retinal ganglion cells in vivo.
2001,
Pubmed
Ruchhoeft,
The neuronal architecture of Xenopus retinal ganglion cells is sculpted by rho-family GTPases in vivo.
1999,
Pubmed
,
Xenbase
Sanchez,
BDNF increases synapse density in dendrites of developing tectal neurons in vivo.
2006,
Pubmed
,
Xenbase
Schikorski,
Morphological correlates of functionally defined synaptic vesicle populations.
2001,
Pubmed
Shen,
Activity-induced rapid synaptic maturation mediated by presynaptic cdc42 signaling.
2006,
Pubmed
Silver,
TrkB-like immunoreactivity is present on geniculocortical afferents in layer IV of kitten primary visual cortex.
2001,
Pubmed
Spalding,
Anterograde transport and trophic actions of BDNF and NT-4/5 in the developing rat visual system.
2002,
Pubmed
Spalding,
Retinal ganglion cell neurotrophin receptor levels and trophic requirements following target ablation in the neonatal rat.
2005,
Pubmed
Suzuki,
Localization of mRNAs for trkB isoforms and p75 in rat retinal ganglion cells.
1998,
Pubmed
Tokuoka,
Regulation by glycogen synthase kinase-3beta of the arborization field and maturation of retinotectal projection in zebrafish.
2002,
Pubmed
Turner,
Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate.
1994,
Pubmed
,
Xenbase
Tyler,
BDNF enhances quantal neurotransmitter release and increases the number of docked vesicles at the active zones of hippocampal excitatory synapses.
2001,
Pubmed
Vicario-Abejón,
Role of neurotrophins in central synapse formation and stabilization.
2002,
Pubmed
Vizuete,
Potential role of endogenous brain-derived neurotrophic factor in long-term neuronal reorganization of the superior colliculus after bilateral visual deprivation.
2001,
Pubmed
Wang,
Presynaptic neurotrophin-3 increases the number of tectal synapses, vesicle density, and number of docked vesicles in chick embryos.
2003,
Pubmed
Witte,
In vivo observations of timecourse and distribution of morphological dynamics in Xenopus retinotectal axon arbors.
1996,
Pubmed
,
Xenbase
Woo,
Activation of p75NTR by proBDNF facilitates hippocampal long-term depression.
2005,
Pubmed
Zweifel,
Functions and mechanisms of retrograde neurotrophin signalling.
2005,
Pubmed
von Bartheld,
Neurotrophins in the developing and regenerating visual system.
1998,
Pubmed
