Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nat Neurosci
2006 Oct 01;910:1247-56. doi: 10.1038/nn1775.
Show Gene links
Show Anatomy links
Asymmetrical beta-actin mRNA translation in growth cones mediates attractive turning to netrin-1.
Leung KM
,
van Horck FP
,
Lin AC
,
Allison R
,
Standart N
,
Holt CE
.
???displayArticle.abstract???
Local protein synthesis regulates the turning of growth cones to guidance cues, yet little is known about which proteins are synthesized or how they contribute to directional steering. Here we show that beta-actin mRNA resides in Xenopus laevis retinal growth cones where it binds to the RNA-binding protein Vg1RBP. Netrin-1 induces the movement of Vg1RBP granules into filopodia, suggesting that it may direct the localization and translation of mRNAs in growth cones. Indeed, a gradient of netrin-1 activates a translation initiation regulator, eIF-4E-binding protein 1 (4EBP), asymmetrically and triggers a polarized increase in beta-actin translation on the near side of the growth cone before growth cone turning. Inhibition of beta-actin translation abolishes both the asymmetric rise in beta-actin and attractive, but not repulsive, turning. Our data suggest that newly synthesized beta-actin, concentrated near sites of signal reception, provides the directional bias for polymerizing actin in the direction of an attractive stimulus.
Ando,
An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein.
2002, Pubmed
Ando,
An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein.
2002,
Pubmed
Bassell,
Sorting of beta-actin mRNA and protein to neurites and growth cones in culture.
1998,
Pubmed
Blichenberg,
Identification of a cis-acting dendritic targeting element in MAP2 mRNAs.
1999,
Pubmed
Brunet,
The transcription factor Engrailed-2 guides retinal axons.
2005,
Pubmed
,
Xenbase
Campbell,
Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones.
2001,
Pubmed
,
Xenbase
Campbell,
Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation.
2001,
Pubmed
,
Xenbase
Chang,
Localization of RNAs to the mitochondrial cloud in Xenopus oocytes through entrapment and association with endoplasmic reticulum.
2004,
Pubmed
,
Xenbase
Condeelis,
How and why does beta-actin mRNA target?
2005,
Pubmed
Deiner,
Netrin-1 and DCC mediate axon guidance locally at the optic disc: loss of function leads to optic nerve hypoplasia.
1997,
Pubmed
Eng,
Synthesis of beta-tubulin, actin, and other proteins in axons of sympathetic neurons in compartmented cultures.
1999,
Pubmed
Fan,
Localized collapsing cues can steer growth cones without inducing their full collapse.
1995,
Pubmed
Gebauer,
Molecular mechanisms of translational control.
2004,
Pubmed
Gingras,
Hierarchical phosphorylation of the translation inhibitor 4E-BP1.
2001,
Pubmed
Guirland,
Lipid rafts mediate chemotropic guidance of nerve growth cones.
2004,
Pubmed
,
Xenbase
Hansen,
Prefoldin-nascent chain complexes in the folding of cytoskeletal proteins.
1999,
Pubmed
Henley,
Guiding neuronal growth cones using Ca2+ signals.
2004,
Pubmed
Höpker,
Growth-cone attraction to netrin-1 is converted to repulsion by laminin-1.
1999,
Pubmed
,
Xenbase
Hüttelmaier,
Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1.
2005,
Pubmed
Kanai,
Kinesin transports RNA: isolation and characterization of an RNA-transporting granule.
2004,
Pubmed
Kislauskis,
beta-Actin messenger RNA localization and protein synthesis augment cell motility.
1997,
Pubmed
Kloc,
Mechanisms of subcellular mRNA localization.
2002,
Pubmed
Kuhn,
Myelin and collapsin-1 induce motor neuron growth cone collapse through different pathways: inhibition of collapse by opposing mutants of rac1.
1999,
Pubmed
Lewis,
Nerve growth cone lamellipodia contain two populations of actin filaments that differ in organization and polarity.
1992,
Pubmed
Li,
Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor.
2005,
Pubmed
,
Xenbase
Liu,
Netrin requires focal adhesion kinase and Src family kinases for axon outgrowth and attraction.
2004,
Pubmed
Lohof,
Asymmetric modulation of cytosolic cAMP activity induces growth cone turning.
1992,
Pubmed
,
Xenbase
López de Heredia,
mRNA localization and the cytoskeleton.
2004,
Pubmed
Ming,
Adaptation in the chemotactic guidance of nerve growth cones.
2002,
Pubmed
,
Xenbase
Ming,
Turning by asymmetric actin.
2006,
Pubmed
Oleynikov,
Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization.
2003,
Pubmed
Ooashi,
Cell adhesion molecules regulate Ca2+-mediated steering of growth cones via cyclic AMP and ryanodine receptor type 3.
2005,
Pubmed
Piper,
Signaling mechanisms underlying Slit2-induced collapse of Xenopus retinal growth cones.
2006,
Pubmed
,
Xenbase
Rousseau,
Translation initiation of ornithine decarboxylase and nucleocytoplasmic transport of cyclin D1 mRNA are increased in cells overexpressing eukaryotic initiation factor 4E.
1996,
Pubmed
Shestakova,
The physiological significance of beta -actin mRNA localization in determining cell polarity and directional motility.
2001,
Pubmed
Shirasaki,
Guidance of circumferentially growing axons by netrin-dependent and -independent floor plate chemotropism in the vertebrate brain.
1996,
Pubmed
Sundell,
Requirement of microfilaments in sorting of actin messenger RNA.
1991,
Pubmed
Takei,
Brain-derived neurotrophic factor enhances neuronal translation by activating multiple initiation processes: comparison with the effects of insulin.
2001,
Pubmed
Vignali,
HNF1(beta) is required for mesoderm induction in the Xenopus embryo.
2000,
Pubmed
,
Xenbase
Wang,
Reversible glutathionylation regulates actin polymerization in A431 cells.
2001,
Pubmed
Wen,
A CaMKII/calcineurin switch controls the direction of Ca(2+)-dependent growth cone guidance.
2004,
Pubmed
,
Xenbase
Wilkie,
Regulation of mRNA translation by 5'- and 3'-UTR-binding factors.
2003,
Pubmed
Willis,
Differential transport and local translation of cytoskeletal, injury-response, and neurodegeneration protein mRNAs in axons.
2005,
Pubmed
Wu,
Local translation of RhoA regulates growth cone collapse.
2005,
Pubmed
Yaniv,
The RNA-binding protein Vg1 RBP is required for cell migration during early neural development.
2003,
Pubmed
,
Xenbase
Yisraeli,
VICKZ proteins: a multi-talented family of regulatory RNA-binding proteins.
2005,
Pubmed
Yuan,
Signalling and crosstalk of Rho GTPases in mediating axon guidance.
2003,
Pubmed
,
Xenbase
Zhang,
Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility.
2001,
Pubmed
Zhang,
Neurotrophin regulation of beta-actin mRNA and protein localization within growth cones.
1999,
Pubmed
Zhang,
Vg1 RBP intracellular distribution and evolutionarily conserved expression at multiple stages during development.
1999,
Pubmed
,
Xenbase
Zheng,
Turning of nerve growth cones induced by localized increases in intracellular calcium ions.
2000,
Pubmed
,
Xenbase
de la Torre,
Turning of retinal growth cones in a netrin-1 gradient mediated by the netrin receptor DCC.
1997,
Pubmed
,
Xenbase
