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Multiciliated cells (MCCs) are extremely highly differentiated, presenting >100 cilia and basal bodies. Therefore, MCC fate is thought to be terminal and irreversible. We analyzed how MCCs are removed from the airway-like mucociliary Xenopus epidermis during developmental tissue remodeling. We found that a subset of MCCs undergoes lateral line-induced apoptosis, but that the majority coordinately trans-differentiate into goblet secretory cells. Both processes are dependent on Notch signaling, while the cellular response to Notch is modulated by Jak/STAT, thyroid hormone, and mTOR signaling. At the cellular level, trans-differentiation is executed through the loss of ciliary gene expression, including foxj1 and pcm1, altered proteostasis, cilia retraction, basal body elimination, as well as the initiation of mucus production and secretion. Our work describes two modes for MCC loss during vertebrate development, the signaling regulation of these processes, and demonstrates that even cells with extreme differentiation features can undergo direct fate conversion.
Antoniades,
Making the connection: ciliary adhesion complexes anchor basal bodies to the actin cytoskeleton.
2014, Pubmed
Antoniades,
Making the connection: ciliary adhesion complexes anchor basal bodies to the actin cytoskeleton.
2014,
Pubmed
Boukhalfa,
Interplay between primary cilia, ubiquitin-proteasome system and autophagy.
2019,
Pubmed
Carruthers,
Depletion of the cell-cycle inhibitor p27(Xic1) impairs neuronal differentiation and increases the number of ElrC(+) progenitor cells in Xenopus tropicalis.
2003,
Pubmed
,
Xenbase
Chen,
UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1.
2019,
Pubmed
Cheng,
Molecular aspects of thyroid hormone actions.
2010,
Pubmed
Chitnis,
Primary neurogenesis in Xenopus embryos regulated by a homologue of the Drosophila neurogenic gene Delta.
1995,
Pubmed
,
Xenbase
Chung,
Coordinated genomic control of ciliogenesis and cell movement by RFX2.
2014,
Pubmed
,
Xenbase
Dalle Nogare,
A framework for understanding morphogenesis and migration of the zebrafish posterior Lateral Line primordium.
2017,
Pubmed
Danahay,
Notch2 is required for inflammatory cytokine-driven goblet cell metaplasia in the lung.
2015,
Pubmed
Deblandre,
A two-step mechanism generates the spacing pattern of the ciliated cells in the skin of Xenopus embryos.
1999,
Pubmed
,
Xenbase
Fini,
Thyroid hormone signaling in the Xenopus laevis embryo is functional and susceptible to endocrine disruption.
2012,
Pubmed
,
Xenbase
Gomperts,
IL-13 regulates cilia loss and foxj1 expression in human airway epithelium.
2007,
Pubmed
Guseh,
Notch signaling promotes airway mucous metaplasia and inhibits alveolar development.
2009,
Pubmed
Haas,
ΔN-Tp63 Mediates Wnt/β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia.
2019,
Pubmed
,
Xenbase
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Hogan,
Repair and regeneration of the respiratory system: complexity, plasticity, and mechanisms of lung stem cell function.
2014,
Pubmed
Holdgaard,
Cloud hunting: doryphagy, a form of selective autophagy that degrades centriolar satellites.
2020,
Pubmed
Huber,
Lysosomal signaling in control of degradation pathways.
2016,
Pubmed
Izawa,
Current topics of functional links between primary cilia and cell cycle.
2015,
Pubmed
Joachim,
Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy.
2017,
Pubmed
Klos Dehring,
Deuterosome-mediated centriole biogenesis.
2013,
Pubmed
,
Xenbase
Kocaturk,
Crosstalk Between Mammalian Autophagy and the Ubiquitin-Proteasome System.
2018,
Pubmed
Kolm,
Efficient hormone-inducible protein function in Xenopus laevis.
1995,
Pubmed
,
Xenbase
Lafkas,
Therapeutic antibodies reveal Notch control of transdifferentiation in the adult lung.
2015,
Pubmed
Liang,
Mechanism of ciliary disassembly.
2016,
Pubmed
Merrell,
Adult cell plasticity in vivo: de-differentiation and transdifferentiation are back in style.
2016,
Pubmed
Myers,
Use of small molecule inhibitors of the Wnt and Notch signaling pathways during Xenopus development.
2014,
Pubmed
,
Xenbase
Nishikawa,
Fate of ciliated epidermal cells during early development of Xenopus laevis using whole-mount immunostaining with an antibody against chondroitin 6-sulfate proteoglycan and anti-tubulin: transdifferentiation or metaplasia of amphibian epidermis.
1992,
Pubmed
,
Xenbase
Pan,
Nutrient deprivation and lysosomal stress induce activation of TFEB in retinal pigment epithelial cells.
2019,
Pubmed
Pardo-Saganta,
Ciliated cells of pseudostratified airway epithelium do not become mucous cells after ovalbumin challenge.
2013,
Pubmed
Pardo-Saganta,
Parent stem cells can serve as niches for their daughter cells.
2015,
Pubmed
Paridaen,
Asymmetric inheritance of centrosome-associated primary cilium membrane directs ciliogenesis after cell division.
2013,
Pubmed
Park,
SPDEF regulates goblet cell hyperplasia in the airway epithelium.
2007,
Pubmed
Park,
Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.
2008,
Pubmed
,
Xenbase
Parker,
Chronic IL9 and IL-13 exposure leads to an altered differentiation of ciliated cells in a well-differentiated paediatric bronchial epithelial cell model.
2013,
Pubmed
Pimenta-Marques,
A mechanism for the elimination of the female gamete centrosome in Drosophila melanogaster.
2016,
Pubmed
Quigley,
Rfx2 Stabilizes Foxj1 Binding at Chromatin Loops to Enable Multiciliated Cell Gene Expression.
2017,
Pubmed
,
Xenbase
Rubbini,
Retinoic Acid Signaling Mediates Hair Cell Regeneration by Repressing p27kip and sox2 in Supporting Cells.
2015,
Pubmed
Ruiz García,
Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures.
2019,
Pubmed
Schindelin,
Fiji: an open-source platform for biological-image analysis.
2012,
Pubmed
Schoborg,
Taking Centrioles to the Elimination Round.
2016,
Pubmed
Smith,
Lateral-line neuromast development in Ambystoma mexicanum and a comparison with Rana pipiens.
1988,
Pubmed
Soldner,
Stem Cells, Genome Editing, and the Path to Translational Medicine.
2018,
Pubmed
Stubbs,
Radial intercalation of ciliated cells during Xenopus skin development.
2006,
Pubmed
,
Xenbase
Tang,
Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites.
2013,
Pubmed
Tu,
Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function.
2018,
Pubmed
,
Xenbase
Tyner,
Blocking airway mucous cell metaplasia by inhibiting EGFR antiapoptosis and IL-13 transdifferentiation signals.
2006,
Pubmed
Walentek,
ATP4a is required for Wnt-dependent Foxj1 expression and leftward flow in Xenopus left-right development.
2012,
Pubmed
,
Xenbase
Walentek,
Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis.
2018,
Pubmed
,
Xenbase
Walentek,
What we can learn from a tadpole about ciliopathies and airway diseases: Using systems biology in Xenopus to study cilia and mucociliary epithelia.
2017,
Pubmed
,
Xenbase
Wang,
Reciprocal regulation of cilia and autophagy via the MTOR and proteasome pathways.
2015,
Pubmed
Wang,
PCM1 recruits Plk1 to the pericentriolar matrix to promote primary cilia disassembly before mitotic entry.
2013,
Pubmed
Wang,
Tethering of an E3 ligase by PCM1 regulates the abundance of centrosomal KIAA0586/Talpid3 and promotes ciliogenesis.
2016,
Pubmed
Wells,
Diverse mechanisms for endogenous regeneration and repair in mammalian organs.
2018,
Pubmed
Winklbauer,
Development of the lateral line system in Xenopus.
1989,
Pubmed
,
Xenbase
van Veenendaal,
Embryonic exposure to propylthiouracil disrupts left-right patterning in Xenopus embryos.
2013,
Pubmed
,
Xenbase
