Novoselov VV et al. (2003), Expression zones of three novel genes abut the ...

XB-ART-5388

Gene Expr Patterns 2003 May 01;32:225-30. doi: 10.1016/s1567-133x(02)00077-7.

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Expression zones of three novel genes abut the developing anterior neural plate of Xenopus embryo.

Novoselov VV , Alexandrova EM , Ermakova GV , Zaraisky AG .

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We identified three novel genes that were expressed within the anterior non-neural ectoderm of Xenopus early neurula embryos. The expression of these genes was observed in the different areas complementary to the expression zone of a homeodomain gene Xanf-1 in the anterior neural plate. One of these genes, a Ras-like GTP-ase Ras-dva, marked the anterior placodal ectoderm area; a second, an Agr family homologous gene, XAgr2, was expressed in the anterior-most ectoderm in the cement gland primordium, and a third, novel gene Nlo was expressed in the lateral neural folds. The genes were transiently expressed in the developing cement and hatching gland primordia, and repressed in the mature cement and hatching glands. XAgr2 and Nlo were also expressed in the otic vesicles, and Ras-dva was expressed in the dorso-lateral column of the neural tube.

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Species referenced: Xenopus
Genes referenced: agr2 arsl hesx1 otop1 otop3 ras-dva1

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	Fig. 2. Expression patterns of the novel genes in anterior ectoderm during normal development. XAgr2 expression at the midneurula stage 15 (A), tailbud stages 28 (B), and 32 (C,D: lateral view). At the stage 32, XAgr2 is expressed in the cells surrounding the cement gland and in the otic vesicle (D, arrowhead). Ras-dva expression at the midneurula stage 15 (E), stage 22 (F), and tailbud stage 25 (F,H: lateral view). At stage 25, Ras-dva expression disappears from the center of developing cement gland, remains in the hatching gland, and starts in the lateral parts of neural tube (H, arrowheads). Nlo expression at the midneurula stage 16 (I), tailbud stages 24 (J), and 27 (K,L: lateral view). At stage 24, Nlo is expressed in the hatching gland (J). At stage 27, Nlo is also expressed in the stripe of cells above the cement gland (K,L, arrows). At stage 32, Nlo is expressed in the otic vesicles (L, arrowhead).
	Fig. 3. Sections of the midneurula stage embryos stained for XAgr2 (A,C) and Xanf-1 (B,C) expression. (A) A sagittal section of XAgr2 expression at the midneurula stage. XAgr2 expression is located in the superficial layer of the anterior ectoderm. The arrow indicates the dorsal limit of expression. (B) Xanf-1 expression at the midneurula stage. The arrow indicates the limit of expression in superficial cells, while expression in the deep cells extends more anteriorly. (C) Double in situ hybridization for XAgr2 (shown in red) and Xanf-1 (shown in blue) at the midneurula stage. (D) Scheme of the XAgr2 expression region (shown in red) and the Xanf-1 expression region (shown in blue) in the superficial and deep layers of anterior ectoderm at the midneurula stage.
	Fig. 4. Transverse sections of embryos stained for XAgr2 (A,B), Ras-dva (C), and Nlo (D–F) expression. (A) A transverse section of the stage 32 embryo; XAgr2 is expressed in the ventromedial part of otic vesicle. (B) XAgr2 is expressed in the notochord. (C) Ras-dva is expressed in the dorsolateral parts of neural tube at the stage 25 (arrows). (D) A transverse section of the stage 32 embryo; Nlo is expressed in the dorsolateral part of otic vesicle. Nlo is expressed by (E) the basal cells of dorsal fin, and (F) by migrating neural crest cells.
	agr2 (anterior gradient 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 15 , anterior view, dorsal up.
	agr2 (anterior gradient 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, anterior view, dorsal up.
	agr2 (anterior gradient 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, anterior view, dorsal up.
	agr2 (anterior gradient 2) gene expression in Xenopus laevis embryo, head region, assayed via in situ hybridization, NF stage 32, lateral view, anterior right, dorsal up. Key: arrow points to otic vesicle.
	loc398404 (ras-related protein ras-dva ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 15, anterior view, dorsal up.
	loc398404( ras-related protein ras-dva ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 22, anterior view, dorsal up.
	loc398404( ras-related protein ras-dva ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25, anterior view, dorsal up.
	loc398404 (ras-related protein ras-dva ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25, lateral view, anterior right, dorsal up. Arrows indicated expression in lateral portions of neural tube.
	otop3 ( otopetrin 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 16, anterior view, dorsal up.
	otop3 ( otopetrin 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, anterior view, dorsal up
	otop3 (otopetrin 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, anterior view, dorsal up.
	otop3 (otopetrin 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, lateral view anterior right, dorsal up.