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Cold Spring Harb Protoc
2018 May 01;20185:. doi: 10.1101/pdb.prot097030.
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Fluorescence In Situ Hybridization of Cryosectioned Xenopus Oocytes.
Neil CR
,
Mowry K
.
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Xenopus laevis oocytes are widely used to study mechanisms of RNA function and biogenesis. While the large size of Xenopus oocytes is amenable to both biochemical and imaging approaches, the relative opacity of the yolk-rich cytoplasm has limited high-resolution imaging of endogenous RNAs. Here, we present a protocol that combines multi-probe fluorescence in situ hybridization with cryosectioning to provide a highly sensitive means of imaging endogenous oocyte RNAs.
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