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Moser M
,
Binder O
,
Wu Y
,
Aitsebaomo J
,
Ren R
,
Bode C
,
Bautch VL
,
Conlon FL
,
Patterson C
.
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The development of endothelial cell precursors is essential for vasculogenesis. We screened for differentially expressed transcripts in endothelial cell precursors in developing mouse embryoid bodies. We cloned a complete cDNA encoding a protein that contains an amino-terminal signal peptide, five cysteine-rich domains, a von Willebrand D domain, and a trypsin inhibitor domain. We termed this protein BMPER (bone morphogenetic protein [BMP]-binding endothelial cell precursor-derived regulator). BMPER is specifically expressed in flk-1-positive cells and parallels the time course of flk-1 induction in these cells. In situ hybridization in mouse embryos demonstrates dorsal midline staining and staining of the aorto-gonadal-mesonephric region, which is known to host vascular precursor cells. BMPER is a secreted protein that directly interacts with BMP2, BMP4, and BMP6 and antagonizes BMP4-dependent Smad5 activation. In Xenopus embryos, ventral injection of BMPER mRNA results in axis duplication and downregulation of the expression of Xvent-1 (downstream target of Smad signaling). In an embryoid body differentiation assay, BMP4-dependent differentiation of endothelial cells in embryoid bodies is also antagonized by BMPER. Taken together, our data indicate that BMPER is a novel BMP-binding protein that is expressed by endothelial cell precursors, has BMP-antagonizing activity, and may play a role in endothelial cell differentiation by modulating local BMP activity.
Abreu,
Connective-tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-beta.
2002, Pubmed,
Xenbase
Abreu,
Connective-tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-beta.
2002,
Pubmed
,
Xenbase
Adelman,
The BMP/BMPR/Smad pathway directs expression of the erythroid-specific EKLF and GATA1 transcription factors during embryoid body differentiation in serum-free media.
2002,
Pubmed
Allen,
Two novel type 2N von Willebrand disease-causing mutations that result in defective factor VIII binding, multimerization, and secretion of von Willebrand factor.
2000,
Pubmed
Balemans,
Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators.
2002,
Pubmed
,
Xenbase
Bautch,
Embryonic stem cell differentiation and the vascular lineage.
2002,
Pubmed
Blitz,
Is chordin a long-range- or short-range-acting factor? Roles for BMP1-related metalloproteases in chordin and BMP4 autofeedback loop regulation.
2000,
Pubmed
,
Xenbase
Butler,
Nonradioactive in situ hybridization to xenopus tissue sections.
2001,
Pubmed
,
Xenbase
Byrd,
Hedgehog is required for murine yolk sac angiogenesis.
2002,
Pubmed
Chang,
Smad5 knockout mice die at mid-gestation due to multiple embryonic and extraembryonic defects.
1999,
Pubmed
Cho,
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid.
1991,
Pubmed
,
Xenbase
Coffin,
Angioblast differentiation and morphogenesis of the vascular endothelium in the mouse embryo.
1991,
Pubmed
Coffin,
Endothelial cell origin and migration in embryonic heart and cranial blood vessel development.
1991,
Pubmed
Coffinier,
Neuralin-1 is a novel Chordin-related molecule expressed in the mouse neural plate.
2001,
Pubmed
,
Xenbase
Conley,
Crossveinless 2 contains cysteine-rich domains and is required for high levels of BMP-like activity during the formation of the cross veins in Drosophila.
2000,
Pubmed
Crossley,
Coordinate expression of Fgf8, Otx2, Bmp4, and Shh in the rostral prosencephalon during development of the telencephalic and optic vesicles.
2001,
Pubmed
Dale,
Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development.
1992,
Pubmed
,
Xenbase
Deckers,
Bone morphogenetic proteins stimulate angiogenesis through osteoblast-derived vascular endothelial growth factor A.
2002,
Pubmed
Drake,
Vasculogenesis in the day 6.5 to 9.5 mouse embryo.
2000,
Pubmed
Dyer,
Indian hedgehog activates hematopoiesis and vasculogenesis and can respecify prospective neurectodermal cell fate in the mouse embryo.
2001,
Pubmed
Ema,
Combinatorial effects of Flk1 and Tal1 on vascular and hematopoietic development in the mouse.
2003,
Pubmed
Ferguson,
Localized enhancement and repression of the activity of the TGF-beta family member, decapentaplegic, is necessary for dorsal-ventral pattern formation in the Drosophila embryo.
1992,
Pubmed
Gale,
Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development.
1999,
Pubmed
Gawantka,
Antagonizing the Spemann organizer: role of the homeobox gene Xvent-1.
1995,
Pubmed
,
Xenbase
Ge,
Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand.
1994,
Pubmed
Hemmati-Brivanlou,
Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activity.
1994,
Pubmed
,
Xenbase
Ishida,
Smad6 is a Smad1/5-induced smad inhibitor. Characterization of bone morphogenetic protein-responsive element in the mouse Smad6 promoter.
2000,
Pubmed
Ishisaki,
Human umbilical vein endothelium-derived cells retain potential to differentiate into smooth muscle-like cells.
2003,
Pubmed
Jones,
DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Jonker,
Endoglin expression in early development is associated with vasculogenesis and angiogenesis.
2002,
Pubmed
Jorieux,
A novel mutation in the D3 domain of von Willebrand factor markedly decreases its ability to bind factor VIII and affects its multimerization.
1998,
Pubmed
Jorieux,
Conformational changes in the D' domain of von Willebrand factor induced by CYS 25 and CYS 95 mutations lead to factor VIII binding defect and multimeric impairment.
2000,
Pubmed
Kabrun,
Flk-1 expression defines a population of early embryonic hematopoietic precursors.
1997,
Pubmed
Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase
Kim,
Bmp6 and Bmp7 are required for cushion formation and septation in the developing mouse heart.
2001,
Pubmed
Larraín,
BMP-binding modules in chordin: a model for signalling regulation in the extracellular space.
2000,
Pubmed
,
Xenbase
Larraín,
Proteolytic cleavage of Chordin as a switch for the dual activities of Twisted gastrulation in BMP signaling.
2001,
Pubmed
,
Xenbase
Maeno,
The role of BMP-4 and GATA-2 in the induction and differentiation of hematopoietic mesoderm in Xenopus laevis.
1996,
Pubmed
,
Xenbase
Massagué,
TGFbeta signaling in growth control, cancer, and heritable disorders.
2000,
Pubmed
Matsui,
Xenopus kielin: A dorsalizing factor containing multiple chordin-type repeats secreted from the embryonic midline.
2000,
Pubmed
,
Xenbase
Medvinsky,
An early pre-liver intraembryonic source of CFU-S in the developing mouse.
1993,
Pubmed
Medvinsky,
Definitive hematopoiesis is autonomously initiated by the AGM region.
1996,
Pubmed
Mishina,
Bmpr encodes a type I bone morphogenetic protein receptor that is essential for gastrulation during mouse embryogenesis.
1995,
Pubmed
Nakayama,
Vascular endothelial growth factor synergistically enhances bone morphogenetic protein-4-dependent lymphohematopoietic cell generation from embryonic stem cells in vitro.
2000,
Pubmed
Nakayama,
A novel chordin-like protein inhibitor for bone morphogenetic proteins expressed preferentially in mesenchymal cell lineages.
2001,
Pubmed
,
Xenbase
Nishikawa,
In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos.
1998,
Pubmed
Nishikawa,
A complex linkage in the developmental pathway of endothelial and hematopoietic cells.
2001,
Pubmed
Offermanns,
Vascular system defects and impaired cell chemokinesis as a result of Galpha13 deficiency.
1997,
Pubmed
Oh,
Activin receptor-like kinase 1 modulates transforming growth factor-beta 1 signaling in the regulation of angiogenesis.
2000,
Pubmed
Piccolo,
Dorsoventral patterning in Xenopus: inhibition of ventral signals by direct binding of chordin to BMP-4.
1996,
Pubmed
,
Xenbase
Rosa,
Mix.1, a homeobox mRNA inducible by mesoderm inducers, is expressed mostly in the presumptive endodermal cells of Xenopus embryos.
1989,
Pubmed
,
Xenbase
Ross,
Twisted gastrulation is a conserved extracellular BMP antagonist.
2001,
Pubmed
,
Xenbase
Sasai,
Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes.
1994,
Pubmed
,
Xenbase
Shah,
Alternative neural crest cell fates are instructively promoted by TGFbeta superfamily members.
1996,
Pubmed
Shalaby,
Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice.
1995,
Pubmed
Smith,
Dorsalization and neural induction: properties of the organizer in Xenopus laevis.
1983,
Pubmed
,
Xenbase
Smith,
Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction.
1991,
Pubmed
,
Xenbase
Smith,
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Smith,
Secreted noggin protein mimics the Spemann organizer in dorsalizing Xenopus mesoderm.
1993,
Pubmed
,
Xenbase
St-Jacques,
Molecular characterization and in situ localization of murine endoglin reveal that it is a transforming growth factor-beta binding protein of endothelial and stromal cells.
1994,
Pubmed
Sánchez,
An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors.
1999,
Pubmed
Takashima,
Targeting of both mouse neuropilin-1 and neuropilin-2 genes severely impairs developmental yolk sac and embryonic angiogenesis.
2002,
Pubmed
Urist,
Bone: formation by autoinduction.
1965,
Pubmed
Vittet,
Embryonic stem cells differentiate in vitro to endothelial cells through successive maturation steps.
1996,
Pubmed
Walmsley,
Adult and embryonic blood and endothelium derive from distinct precursor populations which are differentially programmed by BMP in Xenopus.
2002,
Pubmed
,
Xenbase
Wang,
Embryonic stem cell-derived cystic embryoid bodies form vascular channels: an in vitro model of blood vessel development.
1992,
Pubmed
Winnier,
Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse.
1995,
Pubmed
Xanthos,
The roles of three signaling pathways in the formation and function of the Spemann Organizer.
2002,
Pubmed
,
Xenbase
Yamaguchi,
flk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors.
1993,
Pubmed
Yamashita,
Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors.
2000,
Pubmed
Zhang,
Mice deficient for BMP2 are nonviable and have defects in amnion/chorion and cardiac development.
1996,
Pubmed
Zhu,
Type IIA procollagen containing the cysteine-rich amino propeptide is deposited in the extracellular matrix of prechondrogenic tissue and binds to TGF-beta1 and BMP-2.
1999,
Pubmed
