Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1984 Jun 01;36:1371-5. doi: 10.1002/j.1460-2075.1984.tb01979.x.
Show Gene links
Show Anatomy links
Expression of cholinesterase gene(s) in human brain tissues: translational evidence for multiple mRNA species.
Soreq H
,
Zevin-Sonkin D
,
Razon N
.
???displayArticle.abstract???
To resolve the origin(s) of the molecular heterogeneity of human nervous system cholinesterases (ChEs), we used Xenopus oocytes, which produce biologically active ChE when microinjected with unfractionated brain mRNA. The RNA was prepared from primary gliomas, meningiomas and embryonic brain, each of which expresses ChE activity with distinct substrate specificities and molecular forms. Sucrose gradient fractionation of DMSO-denatured mRNA from these sources revealed three size classes of ChE-inducing mRNAs, sedimenting at approximately 32S, 20S and 9S. The amounts of these different classes of ChE-inducing mRNAs varied between the three tissue sources examined. To distinguish between ChEs produced in oocytes and having different substrate specificities, their activity was determined in the presence of selective inhibitors. Both 'true' (acetylcholine hydrolase, EC 3.1.1.7) and 'pseudo' (acylcholine acylhydrolase, EC 3.1.1.8) multimeric cholinesterase activities were found in the mRNA-injected oocytes. Moreover, human brain mRNAs inducing 'true' and 'pseudo' ChE activities had different size distribution, indicating that different mRNAs might be translated into various types of ChEs. These findings imply that the heterogeneity of ChEs in the human nervous system is not limited to the post-translational level, but extends to the level of mRNA.
AUSTIN,
Two selective inhibitors of cholinesterase.
1953, Pubmed
AUSTIN,
Two selective inhibitors of cholinesterase.
1953,
Pubmed
Edwards,
Divergent regulation of acetylcholinesterase and butyrylcholinesterase in tissues of the rat.
1982,
Pubmed
Eliyahu,
Degranulation of rat cerebellum induces selective variations in gene expression.
1982,
Pubmed
Evans,
RNA processing regulation of neuroendorcrine gene expression.
1982,
Pubmed
Graybiel,
Pseudocholinesterase staining in the primary visual pathway of the macaque monkey.
1982,
Pubmed
Hall,
Multiple forms of acetylcholinesterase and their distribution in endplate and non-endplate regions of rat diaphragm muscle.
1973,
Pubmed
Jedrzejczyk,
Molecular forms of the cholinesterases inside and outside muscle endplates.
1981,
Pubmed
Johnson,
A rapid, simple radiometric assay for cholinesterase, suitable for multiple determinations.
1975,
Pubmed
Koelle,
Effect of protection of butyrylcholinesterase on regeneration of ganglionic acetylcholinesterase.
1977,
Pubmed
Labarca,
MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Lane,
The fate of genes, messengers, and proteins introduced into Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Layer,
Comparative localization of acetylcholinesterase and pseudocholinesterase during morphogenesis of the chicken brain.
1983,
Pubmed
Libermann,
Expression of epidermal growth factor receptors in human brain tumors.
1984,
Pubmed
Long,
Repeated genes in eukaryotes.
1980,
Pubmed
,
Xenbase
Massoulié,
The molecular forms of cholinesterase and acetylcholinesterase in vertebrates.
1982,
Pubmed
Meedel,
Development of translationally active mRNA for larval muscle acetylcholinesterase during ascidian embryogenesis.
1983,
Pubmed
,
Xenbase
Miskin,
Microinjected Xenopus oocytes synthesize active human plasminogen activator.
1981,
Pubmed
,
Xenbase
Richter,
The recruitment of membrane-bound mRNAs for translation in microinjected Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Rieger,
Molecular forms of acetylcholinesterase: their de novo synthesis in mouse neuroblastoma cells.
1976,
Pubmed
Rosenfeld,
Altered expression of the calcitonin gene associated with RNA polymorphism.
1981,
Pubmed
Rotundo,
Synthesis, transport and fate of acetylcholinesterase in cultured chick embryos muscle cells.
1980,
Pubmed
Rotundo,
Synthesis, transport, and fate of acetylcholinesterase and acetylcholine receptors in cultured muscle.
1982,
Pubmed
Scheller,
A single gene encodes multiple neuropeptides mediating a stereotyped behavior.
1983,
Pubmed
Silman,
Parallel regulation of acetylcholinesterase and pseudocholinesterase in normal, denervated and dystrophic chicken skeletal muscle.
1979,
Pubmed
Soreq,
Secreted proteins in the medium of microinjected Xenopus oocytes are degraded by oocyte proteases.
1981,
Pubmed
,
Xenbase
Soreq,
Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
1982,
Pubmed
,
Xenbase
Vigny,
"Nonspecific" cholinesterase and acetylcholinesterase in rat tissues: molecular forms, structural and catalytic properties, and significance of the two enzyme systems.
1978,
Pubmed
Wilson,
Regulation of newly synthesized acetylcholinesterase in muscle cultures treated with diisopropylfluorophosphate.
1974,
Pubmed
