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Synthesis of hyaluronate in differentiated teratocarcinoma cells. Characterization of the synthase.
Prehm P
.
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Differentiation of teratocarcinoma cells led to induction of hyaluronate synthesis. The synthase was recovered in the membrane fraction of cell lysates. Hyaluronate was synthesized at the membranes and was then released as a soluble product. The synthase could be stimulated by a variety of phosphate esters which prevented the degradation of the substrates UDP-GlcNAc and UDP-GlcA and the release of the growing hyaluronic acid chain from the membrane. Hyaluronidases or oligosaccharides derived from hyaluronate did not affect the synthesis. The chains grew at a rate of 60 repeating units/min. Continuous new chain initiation occurred during prolonged synthesis. Digestion of pulse-chase-labelled hyaluronate with beta-N-acetylglucosaminidase and beta-glucuronidase showed that the chains grew at the reducing end.
Appel,
Cell-free synthesis of hyaluronic acid in Marfan syndrome.
1979, Pubmed
Appel,
Cell-free synthesis of hyaluronic acid in Marfan syndrome.
1979,
Pubmed
Hascall,
Aggregation of cartilage proteoglycans. I. The role of hyaluronic acid.
1974,
Pubmed
Hopwood,
Glycosaminoglycan synthesis by cultured human skin fibroblasts after transformation with simian virus 40.
1977,
Pubmed
Hronowski,
The effect of cell density on net rates of glycosaminoglycan synthesis and secretion by cultured rat fibroblasts.
1980,
Pubmed
Kleine,
Biosynthesis of proteoglycans: an approach to locate it in different membrane systems.
1981,
Pubmed
Lindahl,
Biosynthesis of heparin. II. Formation of sulfamino groups.
1973,
Pubmed
Longas,
Sequential hydrolysis of hyaluronate by beta-glucuronidase and beta-N-acetylhexosaminidase.
1981,
Pubmed
Markwell,
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
1978,
Pubmed
Mikuni-Takagaki,
Shedding of hyaluronate from the cell surface of Rous sarcoma virus-transformed chondrocytes.
1979,
Pubmed
Prehm,
Induction of hyaluronic acid synthesis in teratocarcinoma stem cells by retinoic acid.
1980,
Pubmed
Stoolmiller,
The biosynthesis of hyaluronic acid by Streptococcus.
1969,
Pubmed
Sugahara,
Biosynthesis of hyaluronic acid by Streptococcus.
1979,
Pubmed
Tomida,
Induction of hyaluronic acid synthetase activity in rat fibroblasts by medium change of confluent cultures.
1975,
Pubmed
Toole,
Hyaluronate turnover during chondrogenesis in the developing chick limb and axial skeleton.
1972,
Pubmed
Toole,
Developmental roles of hyaluronate and chondroitin sulfate proteoglycans.
1977,
Pubmed
Toole,
Hyaluronate and invasiveness of the rabbit V2 carcinoma.
1979,
Pubmed
