Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-1787
Biochemistry 2005 Jun 14;4423:8274-81. doi: 10.1021/bi050386w.
Show Gene links Show Anatomy links

Nucleoplasmin-mediated unfolding of chromatin involves the displacement of linker-associated chromatin proteins.

Ramos I , Prado A , Finn RM , Muga A , Ausió J .


???displayArticle.abstract???
We have previously characterized the interaction of nucleoplasmin with core histones and studied the possible involvement of this chaperone molecule in transcription. Here we study the interaction of nucleoplasmin with chromatin. We show that highly phosphorylated Xenopus laevis egg nucleoplasmin can unfold sperm and somatic chromatin in a way that involves the removal of chromosomal proteins from linker DNA regions without a stable interaction with the nucleosome. The complexes between egg nucleoplasmin and both somatic and sperm-specific linker proteins have been hydrodynamically characterized using sedimentation equilibrium in the analytical ultracentrifuge. The results are discussed within the context of the possible implication of nucleoplasmin in processes such as transcription and replication licensing which take place after egg fertilization at the onset of development.

???displayArticle.pubmedLink??? 15938617
???displayArticle.link??? Biochemistry


Species referenced: Xenopus laevis
Genes referenced: npm1