XB-ART-16101
J Exp Zool
1997 Aug 15;2786:422-8.
Show Gene links
Show Anatomy links
Involvement of chloride channels in progesterone production during meiotic maturation of follicle-enclosed oocytes of Rana temporaria and Xenopus laevis.
???displayArticle.abstract???
The chloride channel blockers SITS (4-acetamido-4'-isothiocyanostibene-2,2'-disulphonic acid) and DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonic acid) markedly suppressed progesterone production in the Rana temporaria and Xenopus laevis follicle-enclosed oocytes and oocyte maturation stimulated by the homologous pituitary suspension and hCG, respectively. Inhibition was dose-dependent and decreased with the increase of the hormone concentration. SITS did not affect progesterone production in the R. temporaria follicle-enclosed oocytes stimulated by dbcAMP. Substitution of sodium chloride for equimolar concentrations of sodium gluconate, methanesulfonate, glutamate, or formate significantly potentiated the gonadotropin-stimulated progesterone production and oocyte maturation in the both species. Possible involvement of chloride channels in progesterone production by the gonadotropin-stimulated amphibian follicle-enclosed oocytes is discussed.
???displayArticle.pubmedLink??? 9262009
???displayArticle.link??? J Exp Zool