Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2007 Jun 20;2612:2942-54. doi: 10.1038/sj.emboj.7601736.
Show Gene links
Show Anatomy links
POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos.
Cao Y
,
Siegel D
,
Donow C
,
Knöchel S
,
Yuan L
,
Knöchel W
.
???displayArticle.abstract??? VegT and beta-Catenin are key players in the hierarchy of factors that are required for induction and patterning of mesendoderm in Xenopus embryogenesis. By descending the genetic cascades, cells lose their pluripotent status and are determined to differentiate into distinct tissues. Mammalian Oct-3/4, a POU factor of subclass V (POU-V), is required for the maintenance of pluripotency of embryonic stem cells. However, its molecular function within the early embryo is yet poorly understood. We here show that the two maternal Xenopus POU-V factors, Oct-60 and Oct-25, inhibit transcription of genes activated by VegT and beta-Catenin. Maternal POU-V factors and maternal VegT show an opposite distribution along the animal/vegetal axis. Oct-25, VegT and Tcf3 interact with each other and form repression complexes on promoters of VegT and beta-Catenin target genes. We suggest that POU-V factors antagonize primary inducers to allow germ layer specification in a temporally and spatially coordinated manner.
Agius,
Endodermal Nodal-related signals and mesoderm induction in Xenopus.
2000, Pubmed,
Xenbase
Agius,
Endodermal Nodal-related signals and mesoderm induction in Xenopus.
2000,
Pubmed
,
Xenbase
Babaie,
Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells.
2007,
Pubmed
Behrens,
Functional interaction of beta-catenin with the transcription factor LEF-1.
1996,
Pubmed
,
Xenbase
Boiani,
Regulatory networks in embryo-derived pluripotent stem cells.
2005,
Pubmed
Brannon,
A beta-catenin/XTcf-3 complex binds to the siamois promoter to regulate dorsal axis specification in Xenopus.
1997,
Pubmed
,
Xenbase
Cao,
The POU factor Oct-25 regulates the Xvent-2B gene and counteracts terminal differentiation in Xenopus embryos.
2004,
Pubmed
,
Xenbase
Cao,
Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation.
2006,
Pubmed
,
Xenbase
Chew,
Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells.
2005,
Pubmed
Clements,
Mode of action of VegT in mesoderm and endoderm formation.
1999,
Pubmed
,
Xenbase
Conlon,
Determinants of T box protein specificity.
2001,
Pubmed
,
Xenbase
De Robertis,
Dorsal-ventral patterning and neural induction in Xenopus embryos.
2004,
Pubmed
,
Xenbase
Fan,
Wnt signaling and transcriptional control of Siamois in Xenopus embryos.
1998,
Pubmed
,
Xenbase
Hay,
Oct-4 knockdown induces similar patterns of endoderm and trophoblast differentiation markers in human and mouse embryonic stem cells.
2004,
Pubmed
Heasman,
Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach.
2000,
Pubmed
,
Xenbase
Heasman,
Maternal determinants of embryonic cell fate.
2006,
Pubmed
,
Xenbase
Henig,
A POU protein regulates mesodermal competence to FGF in Xenopus.
1998,
Pubmed
,
Xenbase
Hilton,
VegT activation of the early zygotic gene Xnr5 requires lifting of Tcf-mediated repression in the Xenopus blastula.
2003,
Pubmed
,
Xenbase
Hinkley,
Sequential expression of multiple POU proteins during amphibian early development.
1992,
Pubmed
,
Xenbase
Houston,
Repression of organizer genes in dorsal and ventral Xenopus cells mediated by maternal XTcf3.
2002,
Pubmed
,
Xenbase
Hurlstone,
T-cell factors: turn-ons and turn-offs.
2002,
Pubmed
Hyde,
Regulation of the early expression of the Xenopus nodal-related 1 gene, Xnr1.
2000,
Pubmed
,
Xenbase
Ivanova,
Dissecting self-renewal in stem cells with RNA interference.
2006,
Pubmed
Kavka,
Evidence for dual mechanisms of mesoderm establishment in Xenopus embryos.
2000,
Pubmed
,
Xenbase
Kofron,
Mesoderm induction in Xenopus is a zygotic event regulated by maternal VegT via TGFbeta growth factors.
1999,
Pubmed
,
Xenbase
Molenaar,
XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.
1996,
Pubmed
,
Xenbase
Morrison,
Conserved roles for Oct4 homologues in maintaining multipotency during early vertebrate development.
2006,
Pubmed
,
Xenbase
Nichols,
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
1998,
Pubmed
Niwa,
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.
2000,
Pubmed
Niwa,
Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.
2002,
Pubmed
Rex,
Multiple interactions between maternally-activated signalling pathways control Xenopus nodal-related genes.
2002,
Pubmed
,
Xenbase
Roël,
Lef-1 and Tcf-3 transcription factors mediate tissue-specific Wnt signaling during Xenopus development.
2002,
Pubmed
,
Xenbase
Shivdasani,
Molecular regulation of vertebrate early endoderm development.
2002,
Pubmed
,
Xenbase
Snir,
Xenopus laevis POU91 protein, an Oct3/4 homologue, regulates competence transitions from mesoderm to neural cell fates.
2006,
Pubmed
,
Xenbase
Takahashi,
Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center.
2000,
Pubmed
,
Xenbase
Veenstra,
Non-cell autonomous induction of apoptosis and loss of posterior structures by activation domain-specific interactions of Oct-1 in the Xenopus embryo.
1998,
Pubmed
,
Xenbase
Wessely,
Neural induction in the absence of mesoderm: beta-catenin-dependent expression of secreted BMP antagonists at the blastula stage in Xenopus.
2001,
Pubmed
,
Xenbase
White,
Direct and indirect regulation of derrière, a Xenopus mesoderm-inducing factor, by VegT.
2002,
Pubmed
,
Xenbase
Whitfield,
XLPOU-60, a Xenopus POU-domain mRNA, is oocyte-specific from very early stages of oogenesis, and localised to presumptive mesoderm and ectoderm in the blastula.
1993,
Pubmed
,
Xenbase
Whitfield,
Early embryonic expression of XLPOU-60, a Xenopus POU-domain protein.
1995,
Pubmed
,
Xenbase
Wodarz,
Mechanisms of Wnt signaling in development.
1998,
Pubmed
,
Xenbase
Xanthos,
Maternal VegT is the initiator of a molecular network specifying endoderm in Xenopus laevis.
2001,
Pubmed
,
Xenbase
Xanthos,
The roles of three signaling pathways in the formation and function of the Spemann Organizer.
2002,
Pubmed
,
Xenbase
Zhang,
The role of maternal VegT in establishing the primary germ layers in Xenopus embryos.
1998,
Pubmed
,
Xenbase
