XB-ART-28979
Dev Biol
1985 Dec 01;1122:451-7.
Show Gene links
Show Anatomy links
Changes in RNA titers and polyadenylation during oogenesis and oocyte maturation in Xenopus laevis.
???displayArticle.abstract???
The titers of over 90 sequences isolated by cDNA cloning of oocyte poly(A)+RNA were examined during oogenesis in Xenopus laevis. The relative titers of most sequences in unfertilized eggs are established in pre-lamp brush oocytes and persist throughout oogenesis. We have identified several sequences whose titers decrease significantly during the growth phase of oogenesis as well as a few sequences whose titers increase slightly during this period. Among 21 sequences analyzed by RNA gel blots, all remained unchanged in titer during oocyte maturation. A significant fraction of early oocyte RNA does not bind to oligo(dT)cellulose, but by the end of oogenesis transcripts for many RNA species examined are detected exclusively in the poly(A)+RNA fraction. During oocyte maturation a slight size shift or a broadening of the hybridizing band can be seen for many sequences, indicative of poly(A) elongation or degradation.
???displayArticle.pubmedLink??? 2416617
???displayArticle.link??? Dev Biol
???displayArticle.grants???