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The poly(A)(+)-binding protein (PABP) is a highly conserved protein that binds to the poly(A)+ tail of mRNAs. PABP has been shown to regulate message stability and translational efficiency, yet the mechanisms remain unknown. To facilitate further dissection of the functions of this protein, we have expressed and purified Xenopus PABP using a baculovirus expression system. At 48 h after infection of insect Spodoptera frugiperda (Sf9) cells with recombinant virus, approx. 3% of cell protein was PABP. Purification of PABP was achieved by affinity chromatography on poly(A)(+)-Sepharose. The purified protein was indistinguishable from Xenopus PABP with respect to its immunoreactivity and electrophoretic mobility. Furthermore, the recombinant PABP was expressed and purified as a functional protein as indicated by its ability to bind to poly(A)(+)-Sepharose and its ability to enhance the translation of adenylated messages in vitro. By comparing protein extracts from various developmental stages of Xenopus embryos with known amounts of purified PABP, we determined the amount of PABP per embryo. This analysis suggested that there is less than one PABP molecule available per PABP-binding site at early stages of development, and only a slight excess of PABP at later stages.
Bandziulis,
RNA-binding proteins as developmental regulators.
1989, Pubmed
Bandziulis,
RNA-binding proteins as developmental regulators.
1989,
Pubmed
Bernstein,
The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.
1989,
Pubmed
Burd,
The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities.
1991,
Pubmed
,
Xenbase
Christian,
Overexpression of wild-type and dominant negative mutant vimentin subunits in developing Xenopus embryos.
1990,
Pubmed
,
Xenbase
Drawbridge,
Identification and characterization of the poly(A)-binding proteins from the sea urchin: a quantitative analysis.
1990,
Pubmed
Fukami,
In vitro translation of globin: effect of proteins purified by affinity chromatography on polyadenylate-Sepharose.
1976,
Pubmed
Gallie,
The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency.
1991,
Pubmed
Grange,
Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.
1987,
Pubmed
,
Xenbase
Grossi de Sa,
The poly(A)-binding protein facilitates in vitro translation of poly(A)-rich mRNA.
1988,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Lefrère,
Drosophila melanogaster poly(A)-binding protein: cDNA cloning reveals an unusually long 3'-untranslated region of the mRNA, also present in other eukaryotic species [corrected].
1990,
Pubmed
Lorenz,
A cDNA clone for a polyadenylated RNA-binding protein of Xenopus laevis oocytes hybridizes to four developmentally regulated mRNAs.
1985,
Pubmed
,
Xenbase
Luckow,
High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors.
1989,
Pubmed
Munroe,
Tales of poly(A): a review.
1990,
Pubmed
Nietfeld,
The Xenopus laevis poly(A) binding protein is composed of multiple functionally independent RNA binding domains.
1990,
Pubmed
,
Xenbase
Richter,
Developmentally regulated RNA binding proteins during oogenesis in Xenopus laevis.
1983,
Pubmed
,
Xenbase
Sachs,
Purification and characterization of polyadenylate-binding protein.
1990,
Pubmed
Sachs,
The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation.
1989,
Pubmed
Sachs,
A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.
1987,
Pubmed
Sachs,
A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
1986,
Pubmed
Sagata,
A study on the steady-state population of poly(A)+RNA during early development of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Swiderski,
Photocrosslinking of proteins to maternal mRNA in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Thomas,
Translational control of mRNA expression during the early mitogenic response in Swiss mouse 3T3 cells: identification of specific proteins.
1986,
Pubmed
Vissavajjhala,
Purification and characterization of the recombinant extracellular domain of human nerve growth factor receptor expressed in a baculovirus system.
1990,
Pubmed
Zelus,
Expression of the poly(A)-binding protein during development of Xenopus laevis.
1989,
Pubmed
,
Xenbase
