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The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes.
Körner CG
,
Wormington M
,
Muckenthaler M
,
Schneider S
,
Dehlin E
,
Wahle E
.
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Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. We previously described the purification of a poly(A)-specific 3'-exoribonuclease (deadenylating nuclease, DAN) from mammalian tissue. Here, the isolation and functional characterization of cDNA clones encoding human DAN is reported. Recombinant DAN overexpressed in Escherichia coli has properties similar to those of the authentic protein. The amino acid sequence of DAN shows homology to the RNase D family of 3'-exonucleases. DAN appears to be localized in both the nucleus and the cytoplasm. It is not stably associated with polysomes or ribosomal subunits. Xenopus oocytes contain nuclear and cytoplasmic DAN isoforms, both of which are closely related to the human DAN. Anti-DAN antibody microinjected into oocytes inhibits default deadenylation during progesterone-induced maturation. Ectopic expression of human DAN in enucleated oocytes rescues maturation-specific deadenylation, indicating that amphibian and mammalian DANs are functionally equivalent.
Anderson,
The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex.
1998, Pubmed
Anderson,
The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex.
1998,
Pubmed
Audic,
Postfertilization deadenylation of mRNAs in Xenopus laevis embryos is sufficient to cause their degradation at the blastula stage.
1997,
Pubmed
,
Xenbase
Ballantyne,
Poly (A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic changes during oocyte maturation and early development.
1995,
Pubmed
,
Xenbase
Barnes,
The 3'-5' exonuclease site of DNA polymerase III from gram-positive bacteria: definition of a novel motif structure.
1995,
Pubmed
Bashkirov,
A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4 tetraplex substrates.
1997,
Pubmed
Beelman,
An essential component of the decapping enzyme required for normal rates of mRNA turnover.
1996,
Pubmed
Beelman,
Degradation of mRNA in eukaryotes.
1995,
Pubmed
Bernad,
A conserved 3'----5' exonuclease active site in prokaryotic and eukaryotic DNA polymerases.
1989,
Pubmed
Bernstein,
The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.
1989,
Pubmed
Boeck,
The yeast Pan2 protein is required for poly(A)-binding protein-stimulated poly(A)-nuclease activity.
1996,
Pubmed
Bouvet,
The deadenylation conferred by the 3' untranslated region of a developmentally controlled mRNA in Xenopus embryos is switched to polyadenylation by deletion of a short sequence element.
1994,
Pubmed
,
Xenbase
Brown,
PAN3 encodes a subunit of the Pab1p-dependent poly(A) nuclease in Saccharomyces cerevisiae.
1996,
Pubmed
Caponigro,
Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation in yeast.
1995,
Pubmed
Caponigro,
Mechanisms and control of mRNA turnover in Saccharomyces cerevisiae.
1996,
Pubmed
Caponigro,
mRNA turnover in yeast promoted by the MATalpha1 instability element.
1996,
Pubmed
Cohen,
Surprises at the 3' end of prokaryotic RNA.
1995,
Pubmed
Couttet,
Messenger RNA deadenylylation precedes decapping in mammalian cells.
1997,
Pubmed
Decker,
A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation.
1993,
Pubmed
Dellavalle,
Preferential deadenylation of Hsp70 mRNA plays a key role in regulating Hsp70 expression in Drosophila melanogaster.
1994,
Pubmed
Eggert,
The glucocorticoid receptor is associated with the RNA-binding nuclear matrix protein hnRNP U.
1997,
Pubmed
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Fan,
AU-rich elements target small nuclear RNAs as well as mRNAs for rapid degradation.
1997,
Pubmed
Fox,
Poly(A) removal during oocyte maturation: a default reaction selectively prevented by specific sequences in the 3' UTR of certain maternal mRNAs.
1990,
Pubmed
,
Xenbase
Görlach,
The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity.
1994,
Pubmed
Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Huang,
Poly(A)- and poly(U)-specific RNA 3' tail shortening by E. coli ribonuclease E.
1998,
Pubmed
Huarte,
Transient translational silencing by reversible mRNA deadenylation.
1992,
Pubmed
Jacobson,
Interrelationships of the pathways of mRNA decay and translation in eukaryotic cells.
1996,
Pubmed
Joyce,
Function and structure relationships in DNA polymerases.
1994,
Pubmed
Klein,
Induction of mesoderm in Xenopus laevis embryos by translation initiation factor 4E.
1994,
Pubmed
,
Xenbase
Körner,
Poly(A) tail shortening by a mammalian poly(A)-specific 3'-exoribonuclease.
1997,
Pubmed
Kozak,
Structural features in eukaryotic mRNAs that modulate the initiation of translation.
1991,
Pubmed
Kyhse-Andersen,
Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lagnado,
AUUUA is not sufficient to promote poly(A) shortening and degradation of an mRNA: the functional sequence within AU-rich elements may be UUAUUUA(U/A)(U/A).
1994,
Pubmed
LaGrandeur,
Isolation and characterization of Dcp1p, the yeast mRNA decapping enzyme.
1998,
Pubmed
Legagneux,
Substrate-specific regulation of RNA deadenylation in Xenopus embryo and activated egg extracts.
1995,
Pubmed
,
Xenbase
Lennon,
The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression.
1996,
Pubmed
Lisitsky,
The mechanism of preferential degradation of polyadenylated RNA in the chloroplast. The exoribonuclease 100RNP/polynucleotide phosphorylase displays high binding affinity for poly(A) sequence.
1997,
Pubmed
Lowell,
3'-UTR-dependent deadenylation by the yeast poly(A) nuclease.
1992,
Pubmed
Martin,
Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases.
1996,
Pubmed
,
Xenbase
Mian,
Comparative sequence analysis of ribonucleases HII, III, II PH and D.
1997,
Pubmed
Morales,
Destabilization of human alpha-globin mRNA by translation anti-termination is controlled during erythroid differentiation and is paralleled by phased shortening of the poly(A) tail.
1997,
Pubmed
Moser,
The proofreading domain of Escherichia coli DNA polymerase I and other DNA and/or RNA exonuclease domains.
1997,
Pubmed
,
Xenbase
Muckenthaler,
Regulated poly(A) tail shortening in somatic cells mediated by cap-proximal translational repressor proteins and ribosome association.
1997,
Pubmed
Muhlrad,
Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript.
1994,
Pubmed
Muhlrad,
Mutations affecting stability and deadenylation of the yeast MFA2 transcript.
1992,
Pubmed
Nemeth,
Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II.
1995,
Pubmed
O'Hara,
Polyadenylylation helps regulate mRNA decay in Escherichia coli.
1995,
Pubmed
Ozaki,
The genomic analysis of human DAN gene.
1997,
Pubmed
Py,
A DEAD-box RNA helicase in the Escherichia coli RNA degradosome.
1996,
Pubmed
Rogers,
Translation of ferritin light and heavy subunit mRNAs is regulated by intracellular chelatable iron levels in rat hepatoma cells.
1987,
Pubmed
Ross,
Control of messenger RNA stability in higher eukaryotes.
1996,
Pubmed
Ross,
mRNA stability in mammalian cells.
1995,
Pubmed
Sachs,
Starting at the beginning, middle, and end: translation initiation in eukaryotes.
1997,
Pubmed
Sachs,
Translation initiation requires the PAB-dependent poly(A) ribonuclease in yeast.
1992,
Pubmed
Sachs,
The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation.
1989,
Pubmed
Schiavi,
Multiple elements in the c-fos protein-coding region facilitate mRNA deadenylation and decay by a mechanism coupled to translation.
1994,
Pubmed
Shyu,
Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.
1991,
Pubmed
Spector,
Redistribution of U-snRNPs during mitosis.
1986,
Pubmed
Varnum,
Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control.
1990,
Pubmed
,
Xenbase
Varnum,
Maturation-specific deadenylation in Xenopus oocytes requires nuclear and cytoplasmic factors.
1992,
Pubmed
,
Xenbase
Wilson,
Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences.
1988,
Pubmed
Wilson,
The structure of an antigenic determinant in a protein.
1984,
Pubmed
Wormington,
Overexpression of poly(A) binding protein prevents maturation-specific deadenylation and translational inactivation in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Wormington,
Developmental expression and 5S rRNA-binding activity of Xenopus laevis ribosomal protein L5.
1989,
Pubmed
,
Xenbase
Wreden,
Nanos and pumilio establish embryonic polarity in Drosophila by promoting posterior deadenylation of hunchback mRNA.
1997,
Pubmed
Xu,
Modulation of the fate of cytoplasmic mRNA by AU-rich elements: key sequence features controlling mRNA deadenylation and decay.
1997,
Pubmed
Xu,
RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation.
1995,
Pubmed
Zubiaga,
The nonamer UUAUUUAUU is the key AU-rich sequence motif that mediates mRNA degradation.
1995,
Pubmed