Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Curr Biol
2001 May 01;119:671-83. doi: 10.1016/s0960-9822(01)00204-4.
Show Gene links
Show Anatomy links
The schedule of destruction of three mitotic cyclins can dictate the timing of events during exit from mitosis.
Parry DH
,
O'Farrell PH
.
???displayArticle.abstract???
BACKGROUND: Degradation of the mitotic cyclins is a hallmark of the exit from mitosis. Induction of stable versions of each of the three mitotic cyclins of Drosophila, cyclins A, B, and B3, arrests mitosis with different phenotypes. We tested a recent proposal that the destruction of the different cyclins guides progress through mitosis.
RESULTS: Real-time imaging revealed that arrest phenotypes differ because each stable cyclin affects specific mitotic events differently. Stable cyclin A prolonged or blocked chromosome disjunction, leading to metaphase arrest. Stable cyclin B allowed the transition to anaphase, but anaphase A chromosome movements were slowed, anaphase B spindle elongation did not occur, and the monooriented disjoined chromosomes began to oscillate between the spindle poles. Stable cyclin B3 prevented normal spindle maturation and blocked major mitotic exit events such as chromosome decondensation but nonetheless allowed chromosome disjunction, anaphase B, and formation of a cytokinetic furrow, which split the spindle.
CONCLUSIONS: We conclude that degradation of distinct mitotic cyclins is required to transit specific steps of mitosis: cyclin A degradation facilitates chromosome disjunction, cyclin B destruction is required for anaphase B and cytokinesis and for directional stability of univalent chromosome movements, and cyclin B3 degradation is required for proper spindle reorganization and restoration of the interphase nucleus. We suggest that the schedule of degradation of cyclin A, cyclin B, and then cyclin B3 contributes to the temporal coordination of mitotic events.
Clarkson,
A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior.
1999, Pubmed
Clarkson,
A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior.
1999,
Pubmed
Cross,
Conservation and function of a potential substrate-binding domain in the yeast Clb5 B-type cyclin.
2000,
Pubmed
Edgar,
Distinct molecular mechanism regulate cell cycle timing at successive stages of Drosophila embryogenesis.
1994,
Pubmed
Field,
Anillin, a contractile ring protein that cycles from the nucleus to the cell cortex.
1995,
Pubmed
Follette,
Cdks and the Drosophila cell cycle.
1997,
Pubmed
Gallant,
Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells.
1992,
Pubmed
,
Xenbase
Gatti,
Relationships between the central spindle and the contractile ring during cytokinesis in animal cells.
2000,
Pubmed
Ghiara,
A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis.
1991,
Pubmed
Heald,
Motor function in the mitotic spindle.
2000,
Pubmed
Hinchcliffe,
The coordination of centrosome reproduction with nuclear events of the cell cycle in the sea urchin zygote.
1998,
Pubmed
Holloway,
Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
1993,
Pubmed
,
Xenbase
Huang,
The disappearance of cyclin B at the end of mitosis is regulated spatially in Drosophila cells.
1999,
Pubmed
Jacobs,
Drosophila Cyclin B3 is required for female fertility and is dispensable for mitosis like Cyclin B.
1998,
Pubmed
Kaitna,
Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis.
2000,
Pubmed
Knoblich,
Synergistic action of Drosophila cyclins A and B during the G2-M transition.
1993,
Pubmed
Lehner,
Exploring the role of Drosophila cyclin A in the regulation of S phase.
1991,
Pubmed
Lehner,
The roles of Drosophila cyclins A and B in mitotic control.
1990,
Pubmed
Leismann,
Degradation of Drosophila PIM regulates sister chromatid separation during mitosis.
2000,
Pubmed
Luca,
Control of programmed cyclin destruction in a cell-free system.
1989,
Pubmed
Luca,
Both cyclin A delta 60 and B delta 97 are stable and arrest cells in M-phase, but only cyclin B delta 97 turns on cyclin destruction.
1991,
Pubmed
,
Xenbase
Maldonado-Codina,
Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos.
1992,
Pubmed
Meyn,
S-phase cyclins are required for a stable arrest at metaphase.
,
Pubmed
Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
NICKLAS,
Recurrent pole-to-pole movements of the sex chromosome during prometaphase I in Melanoplus differentialis spermatocytes.
1961,
Pubmed
Nasmyth,
Splitting the chromosome: cutting the ties that bind sister chromatids.
2000,
Pubmed
Nicklas,
How cells get the right chromosomes.
1997,
Pubmed
Pines,
Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
1990,
Pubmed
Rimmington,
Expression of N-terminally truncated cyclin B in the Drosophila larval brain leads to mitotic delay at late anaphase.
1994,
Pubmed
Roberts,
Evolving ideas about cyclins.
1999,
Pubmed
Severson,
The aurora-related kinase AIR-2 recruits ZEN-4/CeMKLP1 to the mitotic spindle at metaphase and is required for cytokinesis.
2000,
Pubmed
Sharp,
Functional coordination of three mitotic motors in Drosophila embryos.
2000,
Pubmed
Sigrist,
Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3.
1995,
Pubmed
Sigrist,
Drosophila fizzy-related down-regulates mitotic cyclins and is required for cell proliferation arrest and entry into endocycles.
1997,
Pubmed
,
Xenbase
Sprenger,
S-phase function of Drosophila cyclin A and its downregulation in G1 phase.
1997,
Pubmed
Stern,
Genetic analysis of the Drosophila cdc2 homolog.
1993,
Pubmed
Stratmann,
Separation of sister chromatids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition.
1996,
Pubmed
Su,
DNA damage leads to a Cyclin A-dependent delay in metaphase-anaphase transition in the Drosophila gastrula.
2001,
Pubmed
Su,
Exit from mitosis in Drosophila syncytial embryos requires proteolysis and cyclin degradation, and is associated with localized dephosphorylation.
1998,
Pubmed
Surana,
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.
1993,
Pubmed
Veselý,
Inhibition of cyclin-dependent kinases by purine analogues.
1994,
Pubmed
,
Xenbase
Warren,
Drad21, a Drosophila rad21 homologue expressed in S-phase cells.
2000,
Pubmed
Warren,
The Drosophila RAD21 cohesin persists at the centromere region in mitosis.
2000,
Pubmed
,
Xenbase
Wheatley,
CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo.
1997,
Pubmed
Whitfield,
The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition.
1990,
Pubmed
Zachariae,
Whose end is destruction: cell division and the anaphase-promoting complex.
1999,
Pubmed