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EMBO J
1994 Jan 15;132:349-59. doi: 10.1002/j.1460-2075.1994.tb06268.x.
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Specification of mesodermal pattern in Xenopus laevis by interactions between Brachyury, noggin and Xwnt-8.
Cunliffe V
,
Smith JC
.
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We demonstrate that the nuclear, sequence-specific DNA-binding protein Xbra causes dorsal mesodermal differentiation of animal cap ectoderm when co-expressed with the secreted proteins noggin and Xwnt-8. None of these molecules causes dorsal mesoderm formation when expressed alone. Co-expression of Xenopus Brachyury (Xbra) mRNA with noggin mRNA in animal caps specifies the main dorsal tissues, namely muscle, notochord and neural tissue. Co-expression of Xbra with Xwnt-8, in contrast, converts animal caps to muscle masses. We have previously shown that expression of Xbra alone in animal caps is sufficient to specify ventralmesoderm which expresses Xhox3 and low levels of muscle-specific actin. We now conclude that the putative transcription factor Xbra defines a cell state in the vertebrate embryo which can respond to diffusible dorsal signals such as noggin and Xwnt-8, resulting in dorsal mesodermal differentiation. In the absence of such dorsal signals Xbra causes ventral mesodermal differentiation. This state is only partially maintained after the mid-blastula transition as it permits the dorsal response to zygotically expressed noggin but it does not allow a dorsal response to zygotically expressed Xwnt-8, which elicits only ventral mesodermal differentiation.
Albano,
A mesoderm-inducing factor produced by WEHI-3 murine myelomonocytic leukemia cells is activin A.
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Albano,
A mesoderm-inducing factor produced by WEHI-3 murine myelomonocytic leukemia cells is activin A.
1990,
Pubmed
,
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Almendral,
Complexity of the early genetic response to growth factors in mouse fibroblasts.
1988,
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Asashima,
Presence of activin (erythroid differentiation factor) in unfertilized eggs and blastulae of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Blumberg,
Organizer-specific homeobox genes in Xenopus laevis embryos.
1991,
Pubmed
,
Xenbase
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed
Cho,
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid.
1991,
Pubmed
,
Xenbase
Christian,
Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis.
1991,
Pubmed
,
Xenbase
Christian,
Xwnt-8 modifies the character of mesoderm induced by bFGF in isolated Xenopus ectoderm.
1992,
Pubmed
,
Xenbase
Christian,
Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus.
1993,
Pubmed
,
Xenbase
Cunliffe,
Ectopic mesoderm formation in Xenopus embryos caused by widespread expression of a Brachyury homologue.
1992,
Pubmed
,
Xenbase
Dale,
Secretion and mesoderm-inducing activity of the TGF-beta-related domain of Xenopus Vg1.
1993,
Pubmed
,
Xenbase
Dale,
Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development.
1992,
Pubmed
,
Xenbase
Green,
The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF.
1990,
Pubmed
,
Xenbase
Green,
Responses of embryonic Xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesoderm.
1992,
Pubmed
,
Xenbase
Hausen,
The use of polyacrylamide as an embedding medium for immunohistochemical studies of embryonic tissues.
1981,
Pubmed
Herrmann,
Cloning of the T gene required in mesoderm formation in the mouse.
1990,
Pubmed
Howard,
Are beta 1 integrins involved in Xenopus gastrulation?
1992,
Pubmed
,
Xenbase
Jones,
DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Kimelman,
Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Kintner,
Expression of Xenopus N-CAM RNA in ectoderm is an early response to neural induction.
1987,
Pubmed
,
Xenbase
Kispert,
The Brachyury gene encodes a novel DNA binding protein.
1993,
Pubmed
,
Xenbase
Köster,
Bone morphogenetic protein 4 (BMP-4), a member of the TGF-beta family, in early embryos of Xenopus laevis: analysis of mesoderm inducing activity.
1991,
Pubmed
,
Xenbase
Lamb,
Neural induction by the secreted polypeptide noggin.
1993,
Pubmed
,
Xenbase
Lawson,
Clonal analysis of epiblast fate during germ layer formation in the mouse embryo.
1991,
Pubmed
Lee,
Copper staining: a five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels.
1987,
Pubmed
MacMurray,
The antimorphic nature of the Tc allele at the mouse T locus.
1988,
Pubmed
Niehrs,
The homeobox gene goosecoid controls cell migration in Xenopus embryos.
1993,
Pubmed
,
Xenbase
Rashbass,
A cell autonomous function of Brachyury in T/T embryonic stem cell chimaeras.
1991,
Pubmed
Ruiz i Altaba,
Involvement of the Xenopus homeobox gene Xhox3 in pattern formation along the anterior-posterior axis.
1989,
Pubmed
,
Xenbase
Schulte-Merker,
The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo.
1992,
Pubmed
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Smith,
Mesoderm induction and mesoderm-inducing factors in early amphibian development.
1989,
Pubmed
Smith,
Identification of a potent Xenopus mesoderm-inducing factor as a homologue of activin A.
1990,
Pubmed
,
Xenbase
Smith,
Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction.
1991,
Pubmed
,
Xenbase
Smith,
Injected Xwnt-8 RNA acts early in Xenopus embryos to promote formation of a vegetal dorsalizing center.
1991,
Pubmed
,
Xenbase
Smith,
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Smith,
Secreted noggin protein mimics the Spemann organizer in dorsalizing Xenopus mesoderm.
1993,
Pubmed
,
Xenbase
Smith,
Dorsalization and neural induction: properties of the organizer in Xenopus laevis.
1983,
Pubmed
,
Xenbase
Sokol,
Injected Wnt RNA induces a complete body axis in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Thomsen,
Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structures.
1990,
Pubmed
,
Xenbase
Thomsen,
Processed Vg1 protein is an axial mesoderm inducer in Xenopus.
1993,
Pubmed
,
Xenbase
Wilkinson,
Expression pattern of the mouse T gene and its role in mesoderm formation.
1990,
Pubmed
,
Xenbase
Wilson,
Chimeric analysis of T (Brachyury) gene function.
1993,
Pubmed
Yanagisawa,
Does the T gene determine the anteroposterior axis of a mouse embryo?
1990,
Pubmed
Zhou,
Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation.
1993,
Pubmed
,
Xenbase
van den Eijnden-Van Raaij,
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1990,
Pubmed
,
Xenbase
