Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Nat Genet 2008 Jul 01;407:871-9. doi: 10.1038/ng.104.
Show Gene links Show Anatomy links

Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.

Park TJ , Mitchell BJ , Abitua PB , Kintner C , Wallingford JB .

The planar cell polarity (PCP) signaling system governs many aspects of polarized cell behavior. Here, we use an in vivo model of vertebrate mucociliary epithelial development to show that Dishevelled (Dvl) is essential for the apical positioning of basal bodies. We find that Dvl and Inturned mediate the activation of the Rho GTPase specifically at basal bodies, and that these three proteins together mediate the docking of basal bodies to the apical plasma membrane. Moreover, we find that this docking involves a Dvl-dependent association of basal bodies with membrane-bound vesicles and the vesicle-trafficking protein, Sec8. Once docked, basal bodies again require Dvl and Rho for the planar polarization that underlies directional beating of cilia. These results demonstrate previously undescribed functions for PCP signaling components and suggest that a common signaling apparatus governs both apical docking and planar polarization of basal bodies.

PubMed ID: 18552847
PMC ID: PMC2771675
Article link: Nat Genet
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cetn2 cetn4 dvl1 dvl2 exoc4 intu mapt rho rho.2 rhoa tjp1 tuba8 tubg1
Antibodies: Cetn2 Ab1 Dvl2 Ab2 Dvl3 Ab1 Tjp1 Ab1 Tuba4b Ab2 Tubg1 Ab3
Morpholinos: dvl1 MO1 dvl2 MO1 dvl3 MO1 p2rx1 MO1

Article Images: [+] show captions
References [+] :
Adler, Inturned localizes to the proximal side of wing cells under the instruction of upstream planar polarity proteins. 2004, Pubmed