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Methods Mol Biol
2012 Jan 01;839:119-31. doi: 10.1007/978-1-61779-510-7_10.
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Activation and function of small GTPases Rho, Rac, and Cdc42 during gastrulation.
Mezzacappa C
,
Komiya Y
,
Habas R
.
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Gastrulation is comprised of a series of cell polarization and directional cell migration events that establish the physical body plan of the embryo. One of the major ligand-based pathways that has emerged to play crucial roles in the regulation of gastrulation is the non-canonical Wnt signaling pathway. This aspect of Wnt signaling is comprised of a number of signaling branches that are subsequently integrated for the regulation of changes to the actin cytoskeleton during cell polarization and cell migration during vertebrate gastrulation. The Rho family of small GTPases are activated and required for non-canonical Wnt signaling during gastrulation, and in this chapter, we describe biochemical assays for the detection of Wnt-mediated activation of Rho, Rac, and Cdc42 in both mammalian cells and Xenopus embryo explants.
Akasaki,
Phosphoinositide 3-kinase-dependent and -independent activation of the small GTPase Rac2 in human neutrophils.
1999, Pubmed
Akasaki,
Phosphoinositide 3-kinase-dependent and -independent activation of the small GTPase Rac2 in human neutrophils.
1999,
Pubmed
Benard,
Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases.
1999,
Pubmed
Choi,
Xenopus Cdc42 regulates convergent extension movements during gastrulation through Wnt/Ca2+ signaling pathway.
2002,
Pubmed
,
Xenbase
Endo,
Wnt-3a-dependent cell motility involves RhoA activation and is specifically regulated by dishevelled-2.
2005,
Pubmed
Fuerer,
Wnt signalling in development and disease. Max Delbrück Center for Molecular Medicine meeting on Wnt signaling in Development and Disease.
2008,
Pubmed
Funato,
Nucleoredoxin regulates the Wnt/planar cell polarity pathway in Xenopus.
2008,
Pubmed
,
Xenbase
Giles,
Caught up in a Wnt storm: Wnt signaling in cancer.
2003,
Pubmed
Habas,
Wnt/Frizzled activation of Rho regulates vertebrate gastrulation and requires a novel Formin homology protein Daam1.
2001,
Pubmed
,
Xenbase
Habas,
Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation.
2003,
Pubmed
,
Xenbase
He,
A member of the Frizzled protein family mediating axis induction by Wnt-5A.
1997,
Pubmed
,
Xenbase
He,
LDL receptor-related proteins 5 and 6 in Wnt/beta-catenin signaling: arrows point the way.
2004,
Pubmed
Huang,
The Frizzled family: receptors for multiple signal transduction pathways.
2004,
Pubmed
Jaffe,
Rho GTPases: biochemistry and biology.
2005,
Pubmed
Jessen,
Zebrafish trilobite identifies new roles for Strabismus in gastrulation and neuronal movements.
2002,
Pubmed
Keller,
Shaping the vertebrate body plan by polarized embryonic cell movements.
2002,
Pubmed
Kishida,
Wnt-3a and Dvl induce neurite retraction by activating Rho-associated kinase.
2004,
Pubmed
Kohn,
Wnt and calcium signaling: beta-catenin-independent pathways.
2005,
Pubmed
Komiya,
Wnt signal transduction pathways.
2008,
Pubmed
Lin,
Functions of heparan sulfate proteoglycans in cell signaling during development.
2004,
Pubmed
Logan,
The Wnt signaling pathway in development and disease.
2004,
Pubmed
Lu,
Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth.
2004,
Pubmed
Mlodzik,
Planar cell polarization: do the same mechanisms regulate Drosophila tissue polarity and vertebrate gastrulation?
2002,
Pubmed
Modarresi,
WNT/beta-catenin signaling is involved in regulation of osteoclast differentiation by human immunodeficiency virus protease inhibitor ritonavir: relationship to human immunodeficiency virus-linked bone mineral loss.
2009,
Pubmed
Ohkawara,
Role of glypican 4 in the regulation of convergent extension movements during gastrulation in Xenopus laevis.
2003,
Pubmed
,
Xenbase
Penzo-Mendèz,
Activation of Gbetagamma signaling downstream of Wnt-11/Xfz7 regulates Cdc42 activity during Xenopus gastrulation.
2003,
Pubmed
,
Xenbase
Qiang,
Wnt signaling in B-cell neoplasia.
2003,
Pubmed
Raftopoulou,
Regulation of cell migration by the C2 domain of the tumor suppressor PTEN.
2004,
Pubmed
Raftopoulou,
Cell migration: Rho GTPases lead the way.
2004,
Pubmed
Ren,
Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton.
1999,
Pubmed
Roszko,
Regulation of convergence and extension movements during vertebrate gastrulation by the Wnt/PCP pathway.
2009,
Pubmed
Schlessinger,
Wnt signaling pathways meet Rho GTPases.
2009,
Pubmed
,
Xenbase
Semenov,
SnapShot: Noncanonical Wnt Signaling Pathways.
2007,
Pubmed
Simons,
Electrochemical cues regulate assembly of the Frizzled/Dishevelled complex at the plasma membrane during planar epithelial polarization.
2009,
Pubmed
Simons,
Planar cell polarity signaling: from fly development to human disease.
2008,
Pubmed
Tahinci,
Lrp6 is required for convergent extension during Xenopus gastrulation.
2007,
Pubmed
,
Xenbase
Tanegashima,
WGEF activates Rho in the Wnt-PCP pathway and controls convergent extension in Xenopus gastrulation.
2008,
Pubmed
,
Xenbase
Tao,
A novel G protein-coupled receptor, related to GPR4, is required for assembly of the cortical actin skeleton in early Xenopus embryos.
2005,
Pubmed
,
Xenbase
Topczewski,
The zebrafish glypican knypek controls cell polarity during gastrulation movements of convergent extension.
2001,
Pubmed
Veeman,
Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements.
2003,
Pubmed
Wallingford,
Convergent extension: the molecular control of polarized cell movement during embryonic development.
2002,
Pubmed
,
Xenbase
Wallingford,
Planar cell polarity, ciliogenesis and neural tube defects.
2006,
Pubmed
Wallingford,
The developmental biology of Dishevelled: an enigmatic protein governing cell fate and cell polarity.
2005,
Pubmed
Willert,
A transcriptional response to Wnt protein in human embryonic carcinoma cells.
2002,
Pubmed
Wu,
Rac1 activation controls nuclear localization of beta-catenin during canonical Wnt signaling.
2008,
Pubmed
