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This report describes the isolation and characterization of genomic and cDNA clones which define a subfamily of type I keratins in Xenopus laevis whose expression is restricted to embryonic and larval stages. The XK81 subfamily, named after the prototype cDNA clone DG81, contains four members arranged in two pairs of closely homologous loci; they were named 81A1, A2, B1, and B2. Genomic clones were obtained representing all of these regions. The A1 gene has been completely sequenced together with approximately 1 kb of flanking sequences at each end; this gene corresponds to the previously reported cDNA clone 8128 (Jonas, E., T. D. Sargent, and I. B. Dawid, 1985, Proc. Natl. Acad. Sci. USA, 82:5413-5417). The B2 gene is represented by a partial cDNA clone, DG118. Upstream sequences and about half of the coding regions have been sequenced for the B1 and B2 genes, whereas the A2 locus has been identified on the basis of hybridization data and could be a gene or pseudogene. Genomic Southern blotting indicates that all members of the subfamily have been isolated. The keratin proteins encoded by the B1 and B2 genes are 96% homologous in the central rod domain, whereas A/B gene homology in this region is 81%. During development mRNAs derived from A and B genes accumulate coordinately during gastrula and neurula stages; in the tadpole, 81A mRNA decays rapidly, whereas 81B mRNA shows a second abundance peak, persists for most of tadpole life, and decays by metamorphosis. RNAs derived from the XK81 keratin subfamily are undetectable in the adult, where different type I keratin genes are expressed.
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977, Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Chien,
Isolation and characterization of calmodulin genes from Xenopus laevis.
1984,
Pubmed
,
Xenbase
Ellison,
Developmental changes in keratin patterns during epidermal maturation.
1985,
Pubmed
,
Xenbase
Franz,
Intermediate-size filaments in a germ cell: Expression of cytokeratins in oocytes and eggs of the frog Xenopus.
1983,
Pubmed
,
Xenbase
Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed
Hoffmann,
Amino acid sequence of the carboxy-terminal part of an acidic type I cytokeratin of molecular weight 51 000 from Xenopus laevis epidermis as predicted from the cDNA sequence.
1984,
Pubmed
,
Xenbase
Hoffmann,
Amino acid sequence microheterogeneities of basic (type II) cytokeratins of Xenopus laevis epidermis and evolutionary conservativity of helical and non-helical domains.
1985,
Pubmed
,
Xenbase
Jonas,
Epidermal keratin gene expressed in embryos of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sargent,
Cell interactions and the control of gene activity during early development of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Sargent,
Differential gene expression in the gastrula of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Steinert,
The molecular biology of intermediate filaments.
1985,
Pubmed
Steinert,
Intermediate filaments: conformity and diversity of expression and structure.
1985,
Pubmed
Wahli,
Vitellogenesis and the vitellogenin gene family.
1981,
Pubmed
,
Xenbase
Wahli,
Vitellogenin genes A1 and B1 are linked in the Xenopus laevis genome.
1982,
Pubmed
,
Xenbase
Wallace,
A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322.
1981,
Pubmed
Winkles,
Developmentally regulated cytokeratin gene in Xenopus laevis.
1985,
Pubmed
,
Xenbase
