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Int J Dev Biol
2007 Jan 01;518:745-52. doi: 10.1387/ijdb.072375fn.
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Differential expression of two TEF-1 (TEAD) genes during Xenopus laevis development and in response to inducing factors.
Naye F
,
Tréguer K
,
Soulet F
,
Faucheux C
,
Fédou S
,
Thézé N
,
Thiébaud P
.
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Transcription enhancer factors 1 (TEF-1 or TEAD) make a highly conserved family of eukaryotic DNA binding proteins that activate not only viral regulatory elements but muscle specific genes and are involved in several developmental processes. In this study, we report the identification and the expression pattern of NTEF-1 (TEAD1) and DTEF-1 (TEAD3), two members of this family in Xenopus laevis. Both X. laevis NTEF-1 (XNTEF-1 or XTEAD1) and DTEF-1 (XDTEF-1 or XTEAD3) possess a 72 amino acid TEA domain characteristic of TEF-1 proteins. XNTEF-1 is a 426 amino acid protein that has 96% identity with the avian or the mammalian NTEF-1 proteins while XDTEF-1 is a 433 amino acid protein with 77 to 80% identity with the avian and mammalian DTEF-1 sequences respectively. Temporal expression analysis by RT-PCR indicated that the two genes are expressed maternally and throughout embryonic development. In the adult, the two genes are broadly expressed although they showed differences of expression between tissues. Spatial expression analysis by whole mount in situ hybridization showed that the XNTEF-1 and XDTEF-1 mRNAS were predominantly detected in eye, embryonic brain, somites and heart. In animal cap assay, the two genes are activated by bFGF but are differently regulated by BMP4, and the muscle regulatory factor Mef2d.
Fig. 4. Spatial expression pattern of XNTEF-1 and XDTEF-1 genes in developing embryos.
Whole mount in situ hybridization was performed on embryos at various developmental stages to determine the expression pattern of XNTEF-1 (A-G) and XDTEF-1 (H-N) genes. (A) In stage 17 embryo, XNTEF-1 mRNA is localized in the anterior region of the neural plate covering the eye field (arrowhead). (B) In stage 23 embryo, the expression is found in the eye (ey) and the trunksomites (s). (C) In stage 27 embryo, XNTEF-1 is strongly expressed in the brain (br), the eye (ey) and the trunksomites (s). Black line indicates the position of the section shown in (E). (D) In stage 37 embryo, XNTEF-1 is expressed in the brain, the headmesenchyme, the branchial arches (ba) and the otic vesicle (ov). There is also a widespread expression in the trunk and the posterior region. Black lines indicate the position of the sections shown in (F,G). (E) Transverse section of stage 27 embryo showing XNTEF1 expression in the ventricular zone of the midbrain (mb) and in the eye (ey). (F) Transverse section in the anterior region of stage 37 embryo showed expression in the eye (ey) and in the embryonic heart (h). (G) In transverse section of stage 37 embryo, the staining is present in the hindbrain (hb) and the otic vesicle (ov) but absent from notochord (n). (H) In stage 17 embryo, XDTEF-1 is expressed in midbrain (arrowhead) and paraxial domain (arrow). (I) In stage 27 embryo, the expression is detected in the midbrain and hindbrain regions (arrowheads), the eye (ey), the posteriorsomites and the presomitic mesoderm (psm). (J) In stage 33 embryo, the staining marks predominantly the forebrain (fb), the eye, the branchial arches (ba), the otic vesicle (ov) and the posteriorsomites (s). Black line indicates the position of the section shown in (L). (K) In stage 37 embryo, XDTEF-1 expression is found in the brain, headmesenchyme, otic vesicle (ov), trunk and posterior regions. Black lines indicate the position of the sections shown in (M,N). (L) Transverse section in the head region of stage 33 embryo showed expression in the hindbrain (hb), the otic vesicle (ov) and the branchial arches (ba). (M) Transverse section in the anterior region of stage 37 embryo showed expression in eye (ey), branchial arches (ba) and embryonic heart (h). (N) Transverse section in posterior region of stage 37 embryo showed expression in the somites (s).
tead1 (TEA domain family member 1 {SV40 transcriptional enhancer factor}) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 17, anterior view, dorsal up.
tead1 (TEA domain family member 1 {SV40 transcriptional enhancer factor}) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 23, later view, anteriorleft, dorsal up.
tead1 (TEA domain family member 1 {SV40 transcriptional enhancer factor}) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, lateral view, dorsal up, anteriorleft.
tead1 (TEA domain family member 1 {SV40 transcriptional enhancer factor}) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 37, lateral view, dorsal up, anteriorleft.
tead4 (TEA domain family member 4) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 17, anterior view, dorsal up.
tead4 (TEA domain family member 4) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, lateral view, dorsal up, anteriorleft.
