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EMBO J
1998 Dec 15;1724:7505-13. doi: 10.1093/emboj/17.24.7505.
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Molecular basis of double-stranded RNA-protein interactions: structure of a dsRNA-binding domain complexed with dsRNA.
Ryter JM
,
Schultz SC
.
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Protein interactions with double-stranded RNA (dsRNA) are critical for many cell processes; however, in contrast to protein-dsDNA interactions, surprisingly little is known about the molecular basis of protein-dsRNA interactions. A large and diverse class of proteins that bind dsRNA do so by utilizing an approximately 70 amino acid motif referred to as the dsRNA-binding domain (dsRBD). We have determined a 1.9 A resolution crystal structure of the second dsRBD of Xenopus laevis RNA-binding protein A complexed with dsRNA. The structure shows that the protein spans 16 bp of dsRNA, interacting with two successive minor grooves and across the intervening major groove on one face of a primarily A-form RNA helix. The nature of these interactions explains dsRBD specificity for dsRNA (over ssRNA or dsDNA) and the apparent lack of sequence specificity. Interestingly, the dsRBD fold resembles a portion of the conserved core structure of a family of polynucleotidyl transferases that includes RuvC, MuA transposase, retroviral integrase and RNase H. Structural comparisons of the dsRBD-dsRNA complex and models proposed for polynucleotidyl transferase-nucleic acid complexes suggest that similarities in nucleic acid binding also exist between these families of proteins.
Ariyoshi,
Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli.
1994, Pubmed
Ariyoshi,
Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli.
1994,
Pubmed
Bass,
Binding properties of newly identified Xenopus proteins containing dsRNA-binding motifs.
1994,
Pubmed
,
Xenbase
Bevilacqua,
Minor-groove recognition of double-stranded RNA by the double-stranded RNA-binding domain from the RNA-activated protein kinase PKR.
1996,
Pubmed
Bevilacqua,
Binding of the protein kinase PKR to RNAs with secondary structure defects: role of the tandem A-G mismatch and noncontiguous helixes.
1998,
Pubmed
Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed
Bycroft,
NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5.
1995,
Pubmed
Cerritelli,
The non-RNase H domain of Saccharomyces cerevisiae RNase H1 binds double-stranded RNA: magnesium modulates the switch between double-stranded RNA binding and RNase H activity.
1995,
Pubmed
Cerritelli,
A common 40 amino acid motif in eukaryotic RNases H1 and caulimovirus ORF VI proteins binds to duplex RNAs.
1998,
Pubmed
Chelladurai,
A conserved sequence element in ribonuclease III processing signals is not required for accurate in vitro enzymatic cleavage.
1991,
Pubmed
Chen,
Protein-RNA interactions in an icosahedral virus at 3.0 A resolution.
1989,
Pubmed
Clarke,
Translational control by the Epstein-Barr virus small RNA EBER-1. Reversal of the double-stranded RNA-induced inhibition of protein synthesis in reticulocyte lysates.
1990,
Pubmed
Davies,
Crystal structure of the ribonuclease H domain of HIV-1 reverse transcriptase.
1991,
Pubmed
Dunn,
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.
1983,
Pubmed
Eckmann,
Xlrbpa, a double-stranded RNA-binding protein associated with ribosomes and heterogeneous nuclear RNPs.
1997,
Pubmed
,
Xenbase
Fisher,
Ordered duplex RNA controls capsid architecture in an icosahedral animal virus.
1993,
Pubmed
Galabru,
The binding of double-stranded RNA and adenovirus VAI RNA to the interferon-induced protein kinase.
1989,
Pubmed
Gatignol,
Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR.
1991,
Pubmed
Gibson,
Detection of dsRNA-binding domains in RNA helicase A and Drosophila maleless: implications for monomeric RNA helicases.
1994,
Pubmed
Green,
Two RNA-binding motifs in the double-stranded RNA-activated protein kinase, DAI.
1992,
Pubmed
Jacobo-Molina,
Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA.
1993,
Pubmed
Jones,
Improved methods for building protein models in electron density maps and the location of errors in these models.
1991,
Pubmed
Kharrat,
Structure of the dsRNA binding domain of E. coli RNase III.
1995,
Pubmed
Kim,
Molecular cloning of cDNA for double-stranded RNA adenosine deaminase, a candidate enzyme for nuclear RNA editing.
1994,
Pubmed
Krinke,
The cleavage specificity of RNase III.
1990,
Pubmed
Krovat,
Comparative mutational analysis of the double-stranded RNA binding domains of Xenopus laevis RNA-binding protein A.
1996,
Pubmed
,
Xenbase
Langley,
Recombinant-DNA-derived bovine growth hormone from Escherichia coli. 1. Demonstration that the hormone is expressed in reduced form, and isolation of the hormone in oxidized, native form.
1987,
Pubmed
Larson,
Double-helical RNA in satellite tobacco mosaic virus.
1993,
Pubmed
Larson,
Refined structure of satellite tobacco mosaic virus at 1.8 A resolution.
1998,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Manche,
Interactions between double-stranded RNA regulators and the protein kinase DAI.
1992,
Pubmed
March,
The DNA sequence of the gene (rnc) encoding ribonuclease III of Escherichia coli.
1985,
Pubmed
Mellits,
Interaction of adenovirus VA RNAl with the protein kinase DAI: nonequivalence of binding and function.
1990,
Pubmed
Meurs,
Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon.
1990,
Pubmed
Milligan,
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.
1987,
Pubmed
O'Connell,
Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase.
1995,
Pubmed
Polson,
Preferential selection of adenosines for modification by double-stranded RNA adenosine deaminase.
1994,
Pubmed
Ramakrishnan,
The structure of ribosomal protein S5 reveals sites of interaction with 16S rRNA.
1992,
Pubmed
Rice,
Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration.
1995,
Pubmed
Rould,
Structure of E. coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP at 2.8 A resolution.
1989,
Pubmed
Ruff,
Class II aminoacyl transfer RNA synthetases: crystal structure of yeast aspartyl-tRNA synthetase complexed with tRNA(Asp).
1991,
Pubmed
Schweisguth,
Structural characterization of a ribonuclease III processing signal.
1994,
Pubmed
Sharp,
Comparative analysis of the regulation of the interferon-inducible protein kinase PKR by Epstein-Barr virus RNAs EBER-1 and EBER-2 and adenovirus VAI RNA.
1993,
Pubmed
St Johnston,
Staufen, a gene required to localize maternal RNAs in the Drosophila egg.
1991,
Pubmed
St Johnston,
A conserved double-stranded RNA-binding domain.
1992,
Pubmed
,
Xenbase
Thomis,
Mechanism of interferon action: cDNA structure, expression, and regulation of the interferon-induced, RNA-dependent P1/eIF-2 alpha protein kinase from human cells.
1992,
Pubmed
Wu,
Solution structure of (rGGCAGGCC)2 by two-dimensional NMR and the iterative relaxation matrix approach.
1997,
Pubmed
Yang,
Structure of ribonuclease H phased at 2 A resolution by MAD analysis of the selenomethionyl protein.
1990,
Pubmed
Yang,
Recombining the structures of HIV integrase, RuvC and RNase H.
1995,
Pubmed
