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Proc Natl Acad Sci U S A
2007 Oct 16;10442:16564-9. doi: 10.1073/pnas.0707537104.
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Control of Emi2 activity and stability through Mos-mediated recruitment of PP2A.
Wu JQ
,
Hansen DV
,
Guo Y
,
Wang MZ
,
Tang W
,
Freel CD
,
Tung JJ
,
Jackson PK
,
Kornbluth S
.
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Before fertilization, vertebrate eggs are arrested in meiosis II by cytostatic factor (CSF), which holds the anaphase-promoting complex (APC) in an inactive state. It was recently reported that Mos, an integral component of CSF, acts in part by promoting the Rsk-mediated phosphorylation of the APC inhibitor Emi2/Erp1. We report here that Rsk phosphorylation of Emi2 promotes its interaction with the protein phosphatase PP2A. Emi2 residues adjacent to the Rsk phosphorylation site were important for PP2A binding. An Emi2 mutant that retained Rsk phosphorylation but lacked PP2A binding could not be modulated by Mos. PP2A bound to Emi2 acted on two distinct clusters of sites phosphorylated by Cdc2, one responsible for modulating its stability during CSF arrest and one that controls binding to the APC. These findings provide a molecular mechanism for Mos action in promoting CSF arrest and also define an unusual mechanism, whereby protein phosphorylation recruits a phosphatase for dephosphorylation of distinct sites phosphorylated by another kinase.
Bhatt,
The protein kinase p90 rsk as an essential mediator of cytostatic factor activity.
1999, Pubmed,
Xenbase
Bhatt,
The protein kinase p90 rsk as an essential mediator of cytostatic factor activity.
1999,
Pubmed
,
Xenbase
Casaletto,
Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase.
2005,
Pubmed
,
Xenbase
Gross,
Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90Rsk.
1999,
Pubmed
,
Xenbase
Guadagno,
Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase
Hansen,
Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1.
2004,
Pubmed
,
Xenbase
Hansen,
CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit.
2006,
Pubmed
,
Xenbase
Hansen,
Emi2 at the crossroads: where CSF meets MPF.
2007,
Pubmed
,
Xenbase
Inoue,
A direct link of the Mos-MAPK pathway to Erp1/Emi2 in meiotic arrest of Xenopus laevis eggs.
2007,
Pubmed
,
Xenbase
Liu,
Calcium elevation at fertilization coordinates phosphorylation of XErp1/Emi2 by Plx1 and CaMK II to release metaphase arrest by cytostatic factor.
2005,
Pubmed
,
Xenbase
Liu,
The anaphase-promoting complex/cyclosome inhibitor Emi2 is essential for meiotic but not mitotic cell cycles.
2006,
Pubmed
,
Xenbase
Madgwick,
Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesis.
2006,
Pubmed
Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed
Nishiyama,
Phosphorylation of Erp1 by p90rsk is required for cytostatic factor arrest in Xenopus laevis eggs.
2007,
Pubmed
,
Xenbase
Ohe,
Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes.
2007,
Pubmed
,
Xenbase
Phillips,
Inhibition of MEK or cdc2 kinase parthenogenetically activates mouse eggs and yields the same phenotypes as Mos(-/-) parthenogenotes.
2002,
Pubmed
Rauh,
Calcium triggers exit from meiosis II by targeting the APC/C inhibitor XErp1 for degradation.
2005,
Pubmed
,
Xenbase
Reimann,
Emi1 is required for cytostatic factor arrest in vertebrate eggs.
2002,
Pubmed
,
Xenbase
Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase
Schmidt,
Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity.
2005,
Pubmed
,
Xenbase
Shoji,
Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20.
2006,
Pubmed
,
Xenbase
Tung,
A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs.
2005,
Pubmed
,
Xenbase
Tung,
Translational unmasking of Emi2 directs cytostatic factor arrest in meiosis II.
2007,
Pubmed
,
Xenbase
Tunquist,
The spindle checkpoint kinase bub1 and cyclin e/cdk2 both contribute to the establishment of meiotic metaphase arrest by cytostatic factor.
2002,
Pubmed
,
Xenbase
Vorlaufer,
Regulation of the cyclin B degradation system by an inhibitor of mitotic proteolysis.
1998,
Pubmed
,
Xenbase
Walter,
Activation of Wee1 by p42 MAPK in vitro and in cycling xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Wu,
A role for Cdc2- and PP2A-mediated regulation of Emi2 in the maintenance of CSF arrest.
2007,
Pubmed
,
Xenbase
Yamamoto,
APC/C-Cdc20-mediated degradation of cyclin B participates in CSF arrest in unfertilized Xenopus eggs.
2005,
Pubmed
,
Xenbase
