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Methods Enzymol 2013 Jan 01;525:191-217. doi: 10.1016/B978-0-12-397944-5.00010-9.
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Using Xenopus skin to study cilia development and function.

Werner ME , Mitchell BJ .

Cilia are prevalent biological structures that are important for cell signaling and for generating fluid flow (or motility). Cilia are found throughout biology from single-celled organisms to vertebrates, and many model systems have been employed for their analysis. Here, we describe the use of Xenopus larval skin as a system for the study of ciliogenesis and ciliary function. In particular, we describe basic molecular and embryological manipulations and imaging techniques that have proven particularly useful for understanding the polarized beating of cilia and the generation of directed fluid flow (Werner & Mitchell, 2012). However, these same tools have the potential to benefit a large number of cilia-related biological questions.

PubMed ID: 23522471
PMC ID: PMC4319646
Article link: Methods Enzymol
Grant support: [+]

References [+] :
Amaya, A method for generating transgenic frog embryos. 1999, Pubmed, Xenbase