Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
RNA
2008 Aug 01;148:1459-62. doi: 10.1261/rna.1119708.
Show Gene links
Show Anatomy links
Minor-class splicing occurs in the nucleus of the Xenopus oocyte.
Friend K
,
Kolev NG
,
Shu MD
,
Steitz JA
.
???displayArticle.abstract???
A small fraction of premessenger RNA introns in certain eukaryotes is excised by the minor spliceosome, which contains low-abundance small nuclear ribonucleoproteins (snRNPs). Recently, it was suggested that minor-class snRNPs are localized to and function in the cytoplasm of vertebrate cells. To test whether U12-type splicing occurs in the cytoplasm of Xenopus oocytes, we performed microinjections of the well-characterized P120 minor-class splicing substrate into the nucleus or into the cytoplasm. Our results demonstrate that accurate splicing of this U12-dependent intron occurs exclusively in the nuclear compartment of the oocyte, where U12 and U6atac snRNPs are primarily localized. We further demonstrate that splicing of both a major-class and a minor-class intron is inhibited after nuclear envelope breakdown during meiosis.
Friend,
U2 snRNP binds intronless histone pre-mRNAs to facilitate U7-snRNP-dependent 3' end formation.
2007, Pubmed,
Xenbase
Friend,
U2 snRNP binds intronless histone pre-mRNAs to facilitate U7-snRNP-dependent 3' end formation.
2007,
Pubmed
,
Xenbase
Frilander,
Initial recognition of U12-dependent introns requires both U11/5' splice-site and U12/branchpoint interactions.
1999,
Pubmed
Gall,
Exporting actin.
2006,
Pubmed
,
Xenbase
Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase
Hall,
Conserved sequences in a class of rare eukaryotic nuclear introns with non-consensus splice sites.
1994,
Pubmed
Hall,
Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns.
1996,
Pubmed
König,
Splicing segregation: the minor spliceosome acts outside the nucleus and controls cell proliferation.
2007,
Pubmed
Lorkovic,
Evolutionary conservation of minor U12-type spliceosome between plants and humans.
2005,
Pubmed
Matera,
Nucleoplasmic organization of small nuclear ribonucleoproteins in cultured human cells.
1993,
Pubmed
Mehlin,
Translocation of a specific premessenger ribonucleoprotein particle through the nuclear pore studied with electron microscope tomography.
1992,
Pubmed
Montzka,
Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc.
1988,
Pubmed
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Pan,
Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Pessa,
Minor spliceosome components are predominantly localized in the nucleus.
2008,
Pubmed
Russell,
An early evolutionary origin for the minor spliceosome.
2006,
Pubmed
Sheth,
Comprehensive splice-site analysis using comparative genomics.
2006,
Pubmed
Shin,
The SR protein SRp38 represses splicing in M phase cells.
2002,
Pubmed
Smith,
The induction of oocyte maturation: transmembrane signaling events and regulation of the cell cycle.
1989,
Pubmed
Tarn,
A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro.
1996,
Pubmed
Tarn,
Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns.
1996,
Pubmed
Wang,
Isolation, expression, and characterization of the human ZCRB1 gene mapped to 12q12.
2007,
Pubmed
Wu,
U1-mediated exon definition interactions between AT-AC and GT-AG introns.
1996,
Pubmed
Yang,
Nuclear processing of the 3'-terminal nucleotides of pre-U1 RNA in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Yu,
More Sm snRNAs from vertebrate cells.
1996,
Pubmed
,
Xenbase
Yu,
Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing.
1998,
Pubmed
,
Xenbase
