Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1985 Dec 01;412:3289-97. doi: 10.1002/j.1460-2075.1985.tb04079.x.
Show Gene links
Show Anatomy links
Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease.
Attardi DG
,
Margarit I
,
Tocchini-Valentini GP
.
???displayArticle.abstract???
We have produced a highly purified preparation of the Xenopus laevis splicing endonuclease (XlaI RNase). The purified enzyme correctly cleaves tRNA precursors, creating substrates for subsequent ligation. The 5'-half molecules have a 2',3' cyclic phosphate at their 3' termini. Assuming that splicing enzymes recognize primarily structural elements in the 'mature domain', we have been studying the conformation of three splicing-defective precursors made from mutants of the yeast tRNALeu3 gene. The mutations alter base-pairing in the D-stem region and two of the mutants are absolute defectives. Enzymatic probing of the structures of the altered tRNA precursors shows that the structural perturbations in these mutants are localized on the 'inside' of the 'L'-shaped three-dimensional structure. The implications of this finding for the recognition process are discussed.
Abelson,
RNA processing and the intervening sequence problem.
1979, Pubmed
Abelson,
RNA processing and the intervening sequence problem.
1979,
Pubmed
Akaboshi,
Veal heart ribonuclease P has an essential RNA component.
1980,
Pubmed
Baldi,
Role of RNA structure in splicing: excision of the intervening sequence in yeast tRNA3leu is dependent on the formation of a D stem.
1983,
Pubmed
,
Xenbase
Donis-Keller,
Mapping adenines, guanines, and pyrimidines in RNA.
1977,
Pubmed
Filipowicz,
RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract.
1983,
Pubmed
,
Xenbase
Filipowicz,
Origin of splice junction phosphate in tRNAs processed by HeLa cell extract.
1983,
Pubmed
,
Xenbase
Furneaux,
Isolation and characterization of RNA ligase from wheat germ.
1983,
Pubmed
Johnson,
Transcription and processing of a yeast tRNA gene containing a modified intervening sequence.
1980,
Pubmed
,
Xenbase
Konarska,
Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors.
,
Pubmed
Koontz,
Aminoacyl-tRNA synthetase-catalyzed cleavage of the glycosidic bond of 5-halogenated uridines.
1979,
Pubmed
Lee,
Transfer RNA splicing in Saccharomyces cerevisiae. Secondary and tertiary structures of the substrates.
1985,
Pubmed
Mattoccia,
Mutation in the a block of the yeast tRNAleu3 gene that allows transcription but abolishes splicing and 5'-end maturation.
1983,
Pubmed
Mattoccia,
Separation of RNA transcription and processing activities from X. laevis germinal vesicles.
1979,
Pubmed
,
Xenbase
Nishikura,
Genetic analysis of the processing of a spliced tRNA.
1982,
Pubmed
,
Xenbase
Otsuka,
Ribonuclease "XlaI," an activity from Xenopus laevis oocytes that excises intervening sequences from yeast transfer ribonucleic acid precursors.
1981,
Pubmed
,
Xenbase
Peebles,
Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease.
1983,
Pubmed
Schoemaker,
Effect of aminoacyl transfer RNA synthetases on H-5 exchange of specific pyrimidines in transfer RNAs.
1977,
Pubmed
Starzyk,
Covalent enzyme-RNA complex: a tRNA modification that prevents a covalent enzyme interaction also prevents aminoacylation.
1985,
Pubmed
Starzyk,
A covalent adduct between the uracil ring and the active site of an aminoacyl tRNA synthetase.
1982,
Pubmed
Swerdlow,
Structure of intron-containing tRNA precursors. Analysis of solution conformation using chemical and enzymatic probes.
1984,
Pubmed
Tinoco,
Improved estimation of secondary structure in ribonucleic acids.
1973,
Pubmed
Willis,
Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.
1984,
Pubmed
Wrede,
Conformational changes of yeast tRNAPhe and E. coli tRNA2Glu as indicated by different nuclease digestion patterns.
1979,
Pubmed
