Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1990 Oct 01;8719:7527-31.
Show Gene links
Show Anatomy links
A 140-base-pair repetitive sequence element in the mouse rRNA gene spacer enhances transcription by RNA polymerase I in a cell-free system.
Kuhn A
,
Deppert U
,
Grummt I
.
???displayArticle.abstract???
We show that the repetitive 140-base-pair (bp) elements present in the spacer of mouse rRNA genes function as enhancers for RNA polymerase I. Attachment of these elements to the rDNA promoter stimulates rRNA synthesis both in vivo and in vitro. The cis-activating effect of the spacer repeats is orientation-independent and increases with increasing numbers of the 140-bp elements. Competition experiments demonstrate that the spacer repeats bind one or more of the transcription factors interaction with the rDNA promoter. Both the 140-bp elements and the core promoter act cooperatively and thus are functionally linked. The 60/81-bp enhancer repeats from Xenopus laevis rDNA compete for a murine transcription factor(s) and stimulate transcription often fused to the mouse rDNA promoter. The results indicate that despite the marked species specificity of rDNA transcription initiation, common factors may interact with both the rDNA promoter and the enhancer.
Bell,
Molecular mechanisms governing species-specific transcription of ribosomal RNA.
1989,
Pubmed
,
Xenbase
Clos,
A purified transcription factor (TIF-IB) binds to essential sequences of the mouse rDNA promoter.
1986,
Pubmed
De Winter,
Spacer promoters are essential for efficient enhancement of X. laevis ribosomal transcription.
1986,
Pubmed
,
Xenbase
Dunaway,
Transactivation of the Xenopus rRNA gene promoter by its enhancer.
1989,
Pubmed
,
Xenbase
Dunaway,
A transcription factor, TFIS, interacts with both the promoter and enhancer of the Xenopus rRNA genes.
1989,
Pubmed
,
Xenbase
Grummt,
Structural organization of mouse rDNA: comparison of transcribed and non-transcribed regions.
1980,
Pubmed
Grummt,
Nucleotide sequence requirements for specific initiation of transcription by RNA polymerase I.
1982,
Pubmed
Grummt,
Efficient transcription of a protein-coding gene from the RNA polymerase I promoter in transfected cells.
1985,
Pubmed
Grummt,
A transcription terminator located upstream of the mouse rDNA initiation site affects rRNA synthesis.
1986,
Pubmed
Haltiner,
Two distinct promoter elements in the human rRNA gene identified by linker scanning mutagenesis.
1986,
Pubmed
Henderson,
A transcriptional terminator is a novel element of the promoter of the mouse ribosomal RNA gene.
1986,
Pubmed
Kuehn,
Nucleotide sequence of the genetically labile repeated elements 5' to the origin of mouse rRNA transcription.
1983,
Pubmed
,
Xenbase
Kuhn,
A novel promoter in the mouse rDNA spacer is active in vivo and in vitro.
1987,
Pubmed
Labhart,
Enhancer-like properties of the 60/81 bp elements in the ribosomal gene spacer of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Moss,
A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
,
Pubmed
,
Xenbase
Müeller-Storm,
An enhancer stimulates transcription in trans when attached to the promoter via a protein bridge.
1989,
Pubmed
Pape,
The Xenopus ribosomal DNA 60- and 81-base-pair repeats are position-dependent enhancers that function at the establishment of the preinitiation complex: analysis in vivo and in an enhancer-responsive in vitro system.
1989,
Pubmed
,
Xenbase
Pikaard,
The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF.
1989,
Pubmed
,
Xenbase
Pikaard,
Sequence elements essential for function of the Xenopus laevis ribosomal DNA enhancers.
1988,
Pubmed
,
Xenbase
Reeder,
Spacer regulation of Xenopus ribosomal gene transcription: competition in oocytes.
1983,
Pubmed
,
Xenbase
Schnapp,
Isolation and functional characterization of TIF-IB, a factor that confers promoter specificity to mouse RNA polymerase I.
1990,
Pubmed
Skinner,
In vitro mutagenesis and transcriptional analysis of a mouse ribosomal promoter element.
1984,
Pubmed
Wandelt,
Formation of stable preinitiation complexes is a prerequisite for ribosomal DNA transcription in vitro.
1983,
Pubmed
