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Agonist-specific coupling of a cloned Drosophila melanogaster D1-like dopamine receptor to multiple second messenger pathways by synthetic agonists.
Reale V
,
Hannan F
,
Hall LM
,
Evans PD
.
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The mechanism of coupling of a cloned Drosophila D1-like dopamine receptor, DopR99B, to multiple second messenger systems when expressed in Xenopus oocytes is described. The receptor is coupled directly to the generation of a rapid, transient intracellular Ca2+ signal, monitored as changes in inward current mediated by the oocyte endogenous Ca2+-activated chloride channel, by a pertussis toxin-insensitive G-protein-coupled pathway. The more prolonged receptor-mediated changes in adenylyl cyclase activity are generated by an independent G-protein-coupled pathway that is pertussis toxin-sensitive but calcium-independent, and Gbetagamma-subunits appear to be involved in the transduction of this response. This is the first evidence for the direct coupling of a cloned D1-like dopamine receptor both to the activation of adenylyl cyclase and to the initiation of an intracellular Ca2+ signal. The pharmacological profile of both second messenger effects is identical for a range of naturally occurring catecholamine ligands (dopamine > norepinephrine > epinephrine) and for the blockade of dopamine responses by a range of synthetic antagonists. However, the pharmacological profiles of the two second messenger responses differ for a range of synthetic agonists. Thus, the receptor exhibits agonist-specific coupling to second messenger systems for synthetic agonists. This feature could provide a useful tool in the genetic analysis of the roles of the multiple second messenger pathways activated by this receptor, given the likely involvement of dopamine in the processes of learning and memory in the insect nervous system.
Arakawa,
Cloning, localization, and permanent expression of a Drosophila octopamine receptor.
1990, Pubmed
Arakawa,
Cloning, localization, and permanent expression of a Drosophila octopamine receptor.
1990,
Pubmed
Boekhoff,
Olfactory desensitization requires membrane targeting of receptor kinase mediated by beta gamma-subunits of heterotrimeric G proteins.
1994,
Pubmed
Bouvier,
Dopaminergic activity measured in D1- and D2-transfected fibroblasts by silicon-microphysiometry.
1993,
Pubmed
Buchner,
Genes expressed in the adult brain of Drosophila and effects of their mutations on behavior: a survey of transmitter- and second messenger-related genes.
1991,
Pubmed
Budnik,
Altered branching of serotonin-containing neurons in Drosophila mutants unable to synthesize serotonin and dopamine.
1989,
Pubmed
Budnik,
Catecholamine-containing neurons in Drosophila melanogaster: distribution and development.
1988,
Pubmed
Cooper,
Adenylyl cyclases and the interaction between calcium and cAMP signalling.
1995,
Pubmed
Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986,
Pubmed
,
Xenbase
Davis,
Characterization of receptors mediating the actions of dopamine on an identified inhibitory motoneurone of the cockroach.
1991,
Pubmed
Demchyshyn,
The dopamine D1D receptor. Cloning and characterization of three pharmacologically distinct D1-like receptors from Gallus domesticus.
1995,
Pubmed
,
Xenbase
Eason,
Contribution of ligand structure to activation of alpha 2-adrenergic receptor subtype coupling to Gs.
1994,
Pubmed
Evans,
The action of dopamine receptor antagonists on the secretory response of the cockroach salivary gland in vitro.
1990,
Pubmed
Evans,
Agonist-specific coupling of G-protein-coupled receptors to second-messenger systems.
1995,
Pubmed
Evans,
Characterization of the dopamine receptor mediating the hyperpolarization of cockroach salivary gland acinar cells in vitro.
1990,
Pubmed
Feng,
Cloning and functional characterization of a novel dopamine receptor from Drosophila melanogaster.
1996,
Pubmed
,
Xenbase
Frail,
Cloning and characterization of a truncated dopamine D1 receptor from goldfish retina: stimulation of cyclic AMP production and calcium mobilization.
1993,
Pubmed
Gingrich,
Recent advances in the molecular biology of dopamine receptors.
1993,
Pubmed
Gotzes,
Primary structure and functional characterization of a Drosophila dopamine receptor with high homology to human D1/5 receptors.
1994,
Pubmed
Gurwitz,
Discrete activation of transduction pathways associated with acetylcholine m1 receptor by several muscarinic ligands.
1994,
Pubmed
Han,
DAMB, a novel dopamine receptor expressed specifically in Drosophila mushroom bodies.
1996,
Pubmed
Himmler,
Functional testing of human dopamine D1 and D5 receptors expressed in stable cAMP-responsive luciferase reporter cell lines.
1993,
Pubmed
Inglese,
G beta gamma interactions with PH domains and Ras-MAPK signaling pathways.
1995,
Pubmed
Inglese,
Structure and mechanism of the G protein-coupled receptor kinases.
1993,
Pubmed
Jackson,
Dopamine receptors: molecular biology, biochemistry and behavioural aspects.
1994,
Pubmed
Kenakin,
Agonist-receptor efficacy. II. Agonist trafficking of receptor signals.
1995,
Pubmed
Kenakin,
The classification of seven transmembrane receptors in recombinant expression systems.
1996,
Pubmed
Kimura,
Coupling of human D-1 dopamine receptors to different guanine nucleotide binding proteins. Evidence that D-1 dopamine receptors can couple to both Gs and G(o).
1995,
Pubmed
Koch,
Cellular expression of the carboxyl terminus of a G protein-coupled receptor kinase attenuates G beta gamma-mediated signaling.
1994,
Pubmed
Kokay,
Characterisation of dopamine receptors in insect (Apis mellifera) brain.
1996,
Pubmed
Mahan,
Expression of striatal D1 dopamine receptors coupled to inositol phosphate production and Ca2+ mobilization in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Masu,
cDNA cloning of bovine substance-K receptor through oocyte expression system.
,
Pubmed
,
Xenbase
Ng,
Desensitization, phosphorylation and palmitoylation of the human dopamine D1 receptor.
1994,
Pubmed
Nässel,
Aminergic neurons in the brain of blowflies and Drosophila: dopamine- and tyrosine hydroxylase-immunoreactive neurons and their relationship with putative histaminergic neurons.
1992,
Pubmed
Orr,
Pharmacological characterisation of the dopamine-sensitive adenylate cyclase in cockroach brain: evidence for a distinct dopamine receptor.
1987,
Pubmed
Pedersen,
Characteristics of stably expressed human dopamine D1a and D1b receptors: atypical behavior of the dopamine D1b receptor.
1994,
Pubmed
Quick,
Differential coupling of G protein alpha subunits to seven-helix receptors expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Raymond,
Multiple mechanisms of receptor-G protein signaling specificity.
1995,
Pubmed
Robb,
Agonist-specific coupling of a cloned Drosophila octopamine/tyramine receptor to multiple second messenger systems.
1994,
Pubmed
Saudou,
Cloning and characterization of a Drosophila tyramine receptor.
1990,
Pubmed
Schäfer,
Dopamine-like immunoreactivity in the brain and suboesophageal ganglion of the honeybee.
1989,
Pubmed
Shapira,
Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G alpha q and not G alpha 11.
1994,
Pubmed
,
Xenbase
Sibley,
Molecular biology of dopamine receptors.
1992,
Pubmed
Spengler,
Differential signal transduction by five splice variants of the PACAP receptor.
1993,
Pubmed
,
Xenbase
Stehno-Bittel,
The G protein beta gamma subunit transduces the muscarinic receptor signal for Ca2+ release in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Sugamori,
A primordial dopamine D1-like adenylyl cyclase-linked receptor from Drosophila melanogaster displaying poor affinity for benzazepines.
1995,
Pubmed
Sugamori,
D1A, D1B, and D1C dopamine receptors from Xenopus laevis.
1994,
Pubmed
,
Xenbase
Tamir,
Expression of functional delta opioid receptors in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Tempel,
Mutations in the dopa decarboxylase gene affect learning in Drosophila.
1984,
Pubmed
Van Renterghem,
GABA receptors induced in Xenopus oocytes by chick brain mRNA: evaluation of TBPS as a use-dependent channel-blocker.
1987,
Pubmed
,
Xenbase
White,
Expression of functional pituitary somatostatin receptors in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Yakel,
Functional expression of adenosine A2b receptor in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
de la Peña,
Gs couples thyrotropin-releasing hormone receptors expressed in Xenopus oocytes to phospholipase C.
1995,
Pubmed
,
Xenbase
