Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Methods Mol Biol
2012 Jan 01;917:391-407. doi: 10.1007/978-1-61779-992-1_22.
Show Gene links
Show Anatomy links
Complementary proteomic analysis of protein complexes.
Greco TM
,
Miteva Y
,
Conlon FL
,
Cristea IM
.
???displayArticle.abstract???
Proteomic characterization of protein complexes leverages the versatile platform of liquid chromatography-tandem mass spectrometry to elucidate molecular and cellular signaling processes underlying the dynamic regulation of macromolecular assemblies. Here, we describe a complementary proteomic approach optimized for immunoisolated protein complexes. As the relative complexity, abundance, and physiochemical properties of proteins can vary significantly between samples, we have provided (1) complementary sample preparation workflows, (2) detailed steps for HPLC and mass spectrometric method development, and (3) a bioinformatic workflow that provides confident peptide/protein identification paired with unbiased functional gene ontology analysis. This protocol can also be extended for characterization of larger complexity samples from whole cell or tissue Xenopus proteomes.
Fang,
Affinity separation and enrichment methods in proteomic analysis.
2008, Pubmed
Fang,
Affinity separation and enrichment methods in proteomic analysis.
2008,
Pubmed
Greco,
Nuclear import of histone deacetylase 5 by requisite nuclear localization signal phosphorylation.
2011,
Pubmed
Gygi,
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.
1999,
Pubmed
Hossain,
Enhanced sensitivity for selected reaction monitoring mass spectrometry-based targeted proteomics using a dual stage electrodynamic ion funnel interface.
2011,
Pubmed
Keller,
Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search.
2002,
Pubmed
Kramer,
Proteomic characterization of pseudorabies virus extracellular virions.
2011,
Pubmed
Käll,
Semi-supervised learning for peptide identification from shotgun proteomics datasets.
2007,
Pubmed
Köcher,
Ultra-high-pressure RPLC hyphenated to an LTQ-Orbitrap Velos reveals a linear relation between peak capacity and number of identified peptides.
2011,
Pubmed
Moorman,
A targeted spatial-temporal proteomics approach implicates multiple cellular trafficking pathways in human cytomegalovirus virion maturation.
2010,
Pubmed
Nesvizhskii,
A survey of computational methods and error rate estimation procedures for peptide and protein identification in shotgun proteomics.
2010,
Pubmed
Olsen,
A dual pressure linear ion trap Orbitrap instrument with very high sequencing speed.
2009,
Pubmed
Olsen,
Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
2010,
Pubmed
Picotti,
Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics.
2009,
Pubmed
Selimi,
Proteomic studies of a single CNS synapse type: the parallel fiber/purkinje cell synapse.
2009,
Pubmed
Wang,
Comparison of extensive protein fractionation and repetitive LC-MS/MS analyses on depth of analysis for complex proteomes.
2010,
Pubmed
Washburn,
Large-scale analysis of the yeast proteome by multidimensional protein identification technology.
2001,
Pubmed
Wenger,
COMPASS: a suite of pre- and post-search proteomics software tools for OMSSA.
2011,
Pubmed
